Human Glycogen Synthase Kinase 3 Alpha ELISA Kit | GSK3alpha elisa kit

Human Glycogen Synthase Kinase 3 Alpha ELISA Kit

Cat. Num. AAA764297

• Reactivity: Human
• Synonyms: Glycogen Synthase Kinase 3 Alpha; Human Glycogen Synthase Kinase 3 Alpha ELISA Kit; GSK3alpha/DKFZp686D0638/glycogen synthase kinase 3 alpha/glycogen synthase kinase-3 alpha/GSK-3 alpha ; GSK3alpha elisa kit

• Reactivity: Human
• Sequence Length: 483

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for GSK3alpha elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human GSK3alpha antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human GSK3alpha antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human GSK3alpha amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human GSK3alpha can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 30354008
• NCBI GeneID: 2931
• Molecular Weight: 50,981 Da
• NCBI Official Full Name: Glycogen synthase kinase 3 alpha
• NCBI Official Synonym Full Names: glycogen synthase kinase 3 alpha
• NCBI Official Symbol: GSK3A
• NCBI Protein Information: glycogen synthase kinase-3 alpha
• UniProt Protein Name: Glycogen synthase kinase-3 alpha
• UniProt Gene Name: GSK3A
• UniProt Synonym Gene Names: GSK-3 alpha
• UniProt Entry Name: GSK3A_HUMAN

NCBI Description

This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease. [provided by RefSeq, Oct 2011]

Uniprot Description

GSK3A: a proline-directed protein kinase of the GSK family. Implicated in the control of several regulatory proteins including glycogen synthase, Myb, and c-Jun. GSK3 and GSK3 have similar functions. GSK3 phophorylates tau, the principal component of neurofibrillary tangles in Alzheimer disease and is required for maximal production of amyloid plaque peptides by secretase. A GSK3 promoter SNP effects progression of bipolar disorder. The GSK3 inhibitor, lithium, is used to treat bipolar disorder and is seen to block plaque formation. GSK3 generally opposes the action of insulin, and GSK3 hyperactivity is thought to contribute to insulin resistant (type II) diabetes. GSK3 also negatively regulates cardiac hypertrophy. A tumor suppressor role is indicated by the oncogenic potential of stabilized -catenin mutants that lack GSK3 phosphorylation sites. Inhibitor: AR-A014418

Protein type: EC 2.7.11.1; EC 2.7.11.26; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, CMGC; CMGC group; GSK family; GSK subfamily

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: beta-catenin destruction complex; cytosol; mitochondrion

Molecular Function: ATP binding; protein binding; protein serine/threonine kinase activity; tau-protein kinase activity

Biological Process: aging; cellular protein metabolic process; cellular response to insulin stimulus; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; glycogen metabolic process; innate immune response; insulin receptor signaling pathway; negative regulation of glucose import; negative regulation of glycogen biosynthetic process; negative regulation of insulin receptor signaling pathway; negative regulation of TOR signaling pathway; nerve growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of cAMP biosynthetic process; positive regulation of heart contraction; positive regulation of neuron apoptosis; positive regulation of protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; protein amino acid phosphorylation; regulation of systemic arterial blood pressure; unfolded protein response; Wnt receptor signaling pathway