Typical Testing Data/Standard Curve (for reference only)
Mouse Glycogen synthase kinase-3 beta ELISA Kit | Gsk3b elisa kit
Mouse Glycogen synthase kinase-3 beta ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GSK-3 beta. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forGSK-3 beta and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GSK-3 beta, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of GSK-3 beta in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
GSK3B: a proline-directed protein kinase of the GSK family. Phosphorylates and inactivates glycogen synthase. Participates in the Wnt signaling pathway. Involved in energy metabolism, neuronal cell development, and body pattern formation
Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.26; EC 2.7.11.1; Protein kinase, CMGC; CMGC group; GSK family; GSK subfamily
Cellular Component: axon; beta-catenin destruction complex; cell soma; centrosome; cytoplasm; cytosol; dendrite; dendritic shaft; dendritic spine; growth cone; lipid raft; membrane; microtubule; mitochondrion; nucleus; perinuclear region of cytoplasm; plasma membrane; protein complex; ribonucleoprotein complex
Molecular Function: ATP binding; beta-catenin binding; integrin binding; ionotropic glutamate receptor binding; kinase activity; NF-kappaB binding; nucleotide binding; p53 binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; tau protein binding; tau-protein kinase activity; transcription factor binding; transferase activity; ubiquitin protein ligase binding
Biological Process: axonogenesis; carbohydrate metabolic process; cell differentiation; cell migration; cell proliferation; circadian rhythm; cytoskeleton organization and biogenesis; epithelial to mesenchymal transition; ER overload response; establishment and/or maintenance of cell polarity; establishment of cell polarity; fat cell differentiation; genetic imprinting; glycogen metabolic process; hippocampus development; multicellular organismal development; myoblast fusion; myotube differentiation; negative regulation of apoptosis; negative regulation of dendrite morphogenesis; negative regulation of MAP kinase activity; negative regulation of neuron maturation; negative regulation of NFAT protein import into nucleus; negative regulation of nitric-oxide synthase activity; negative regulation of protein binding; negative regulation of protein complex assembly; negative regulation of signal transduction; nervous system development; organ morphogenesis; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; phosphorylation; positive regulation of apoptosis; positive regulation of axon extension; positive regulation of cell-matrix adhesion; positive regulation of GTPase activity; positive regulation of neuron apoptosis; positive regulation of peptidyl-serine phosphorylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein binding; positive regulation of protein catabolic process; positive regulation of protein complex assembly; positive regulation of protein export from nucleus; positive regulation of smooth muscle cell proliferation; positive regulation of transcription from RNA polymerase II promoter; protein amino acid autophosphorylation; protein amino acid phosphorylation; protein export from nucleus; re-entry into mitotic cell cycle; regulation of axon extension; regulation of axonogenesis; regulation of dendrite morphogenesis; regulation of microtubule-based process; regulation of neuronal synaptic plasticity; rhythmic process; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin
Research Articles on Gsk3b
Similar Products
Product Notes
The Mouse Gsk3b gsk3b (Catalog #AAA2887455) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2887455 ELISA Kit recognizes Mouse Gsk3b. It is sometimes possible for the material contained within the vial of "Glycogen synthase kinase-3 beta, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
