Rabbit Matrix metalloproteinase 2 ELISA Kit | MMP-2 elisa kit

Rabbit Matrix metalloproteinase 2 ELISA Kit

Cat. Num. AAA732397

• Reactivity: Rabbit
• Synonyms: Matrix metalloproteinase 2; Rabbit Matrix metalloproteinase 2 ELISA Kit; MMP-2 elisa kit

• Reactivity: Rabbit
• Specificity: This assay has high sensitivity and excellent specificity for detection of MMP-2. No significant cross-reactivity or interference between MMP-2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MMP-2 and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 1.0 pg/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MMP-2 elisa kit

Intended Uses: This MMP-2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit MMP-2. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: MMP-2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-MMP-2 antibody and an MMP-2-HRP conjugate. The assay sample and buffer are incubated together with MMP-2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MMP-2 concentration since MMP-2 from samples and MMP-2-HRP conjugate compete for the anti-MMP-2 antibody binding site. Since the number of sites is limited, as more sites are occupied by MMP-2 from the sample, fewer sites are left to bind MMP-2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMP-2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for MMP-2 elisa kit

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 8050250
• NCBI GeneID: 81686
• Molecular Weight: 74,149 Da
• NCBI Official Full Name: MMP-2, partial
• NCBI Official Synonym Full Names: matrix metallopeptidase 2
• NCBI Official Symbol: Mmp2
• NCBI Protein Information: 72 kDa type IV collagenase; MMP-2; gelatinase A; 72 kDa gelatinase; matrix metalloproteinase 2; matrix metalloproteinase-2
• UniProt Protein Name: 72 kDa type IV collagenase
• UniProt Gene Name: Mmp2
• UniProt Synonym Gene Names: MMP-2
• UniProt Entry Name: MMP2_RAT

NCBI Description

plays important roles in wound healing, angiogenesis, platelet aggregation, and tumor metastasis; mediates the response of the intrinsic glomerular mesangial cell to inflammatory stimuli [RGD, Feb 2006]

Uniprot Description

Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues ().