Human Tau-Protein Kinase ELISA Kit | tauPK1 elisa kit

Human Tau-Protein Kinase ELISA Kit

Cat. Num. AAA760648

• Reactivity: Human
• Synonyms: Tau-Protein Kinase; Human Tau-Protein Kinase ELISA Kit; tauPK1 ; tauPK1 elisa kit

• Reactivity: Human
• Sequence Length: 420

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for tauPK1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human tauPK1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human tauPK1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human tauPK1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human tauPK1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 402652
• NCBI GeneID: 84027
• UniProt Accession #: P18266
• Molecular Weight: 46,742 Da
• NCBI Official Full Name: tau-protein kinase
• NCBI Official Synonym Full Names: glycogen synthase kinase 3 beta
• NCBI Official Symbol: Gsk3b
• NCBI Protein Information: glycogen synthase kinase-3 beta
• UniProt Protein Name: Glycogen synthase kinase-3 beta
• UniProt Gene Name: Gsk3b
• UniProt Synonym Gene Names: GSK-3 beta; FA
• UniProt Entry Name: GSK3B_RAT

NCBI Description

mediates Par6-atypical protein kinase C (aPKC) complex regulation of cell polarity; may induce apoptosis [RGD, Feb 2006]

Uniprot Description

GSK3B: a proline-directed protein kinase of the GSK family. Phosphorylates and inactivates glycogen synthase. Participates in the Wnt signaling pathway. Involved in energy metabolism, neuronal cell development, and body pattern formation

Protein type: EC 2.7.11.1; EC 2.7.11.26; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; Kinase, protein; CMGC group; GSK family; GSK subfamily

Cellular Component: axon; beta-catenin destruction complex; cell soma; centrosome; cytoplasm; cytosol; dendrite; dendritic shaft; dendritic spine; growth cone; lipid raft; membrane; microtubule; mitochondrion; nucleoplasm; nucleus; perinuclear region of cytoplasm; plasma membrane; protein complex; ribonucleoprotein complex

Molecular Function: ATP binding; beta-catenin binding; integrin binding; ionotropic glutamate receptor binding; kinase activity; NF-kappaB binding; p53 binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; tau protein binding; tau-protein kinase activity; transcription factor binding; ubiquitin protein ligase binding

Biological Process: aging; axonogenesis; cell migration; circadian rhythm; epithelial to mesenchymal transition; ER overload response; establishment and/or maintenance of cell polarity; establishment of cell polarity; fat cell differentiation; genetic imprinting; glycogen metabolic process; hippocampus development; myoblast fusion; myotube differentiation; negative regulation of apoptosis; negative regulation of dendrite morphogenesis; negative regulation of MAP kinase activity; negative regulation of neuron maturation; negative regulation of NFAT protein import into nucleus; negative regulation of nitric-oxide synthase activity; negative regulation of protein binding; negative regulation of protein complex assembly; organ morphogenesis; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; phosphorylation; positive regulation of apoptosis; positive regulation of axon extension; positive regulation of cell-matrix adhesion; positive regulation of GTPase activity; positive regulation of neuron apoptosis; positive regulation of peptidyl-serine phosphorylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein binding; positive regulation of protein catabolic process; positive regulation of protein complex assembly; positive regulation of protein export from nucleus; positive regulation of smooth muscle cell proliferation; positive regulation of transcription from RNA polymerase II promoter; protein amino acid autophosphorylation; protein amino acid phosphorylation; protein export from nucleus; re-entry into mitotic cell cycle; regulation of axon extension; regulation of axonogenesis; regulation of dendrite morphogenesis; regulation of microtubule-based process; regulation of neuronal synaptic plasticity; response to activity; response to drug; response to estradiol stimulus; response to insulin stimulus; response to lithium ion; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin