Western Blot (WB)
(Western Blot analysis of extract of various cells, using UBE2D4 antibody.)
Rabbit UBE2D4 Polyclonal Antibody | anti-UBE2D4 antibody
UBE2D4 Polyclonal Antibody
Gene Names
UBE2D4; HBUCE1
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
UBE2D4; Polyclonal Antibody; UBE2D4 Polyclonal Antibody; HBUCE1; anti-UBE2D4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
UBE2D4 polyclonal antibody detects endogenous levels of UBE2D4 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
147
Applicable Applications for anti-UBE2D4 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
Recombinant protein of human UBE2D4.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot analysis of extract of various cells, using UBE2D4 antibody.)
Western Blot (WB)
(Western Blot analysis of extract of various cells, using UBE2D4 antibody.)
Related Product Information for anti-UBE2D4 antibody
Ubiquitin is an abundant, highly conserved protein found in all eukaryotic cells either free, or covalently attached, to cellular proteins. The primary function of ubiquitin in mammalian systems is to clear abnormal, foreign and improperly folded proteins by targeting them for proteosomal degradation. UBE2D proteins, including UBE2D1 (ubiquitin-conjugating enzyme E2D1), UBE2D2 (ubiquitin-conjugating enzyme E2D2), UBE2D3 (ubiquitin-conjugating enzyme E2D3) and UBE2D4 (ubiquitin-conjugating enzyme E2D4, also known as HBUCE1), are E2 ubiquitin-conjugating enzymes that catalyze the ubiquitination of target proteins in an ATP-dependent manner. Specifically, an E1 enzyme first transfers a ubiquitin residue to an E2 component (a UBE2D protein), and the UBE2D protein then associates with an E3 ubiquitin-protein ligase, which immediately transfers that residue to a protein that is targeted for degradation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 17kDa
NCBI Official Full Name
ubiquitin-conjugating enzyme E2 D4
NCBI Official Synonym Full Names
ubiquitin conjugating enzyme E2 D4 (putative)
NCBI Official Symbol
UBE2D4
NCBI Official Synonym Symbols
HBUCE1
NCBI Protein Information
ubiquitin-conjugating enzyme E2 D4
UniProt Protein Name
Ubiquitin-conjugating enzyme E2 D4
Protein Family
UniProt Gene Name
UBE2D4
UniProt Synonym Gene Names
UBCH5D
UniProt Entry Name
UB2D4_HUMAN
Uniprot Description
UBE2D4: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination. Belongs to the ubiquitin-conjugating enzyme family.
Protein type: Ligase; Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.19
Chromosomal Location of Human Ortholog: 7p13
Molecular Function: protein binding; ubiquitin-protein ligase activity; ATP binding; ligase activity
Biological Process: protein ubiquitination
Research Articles on UBE2D4
Similar Products
Product Notes
The UBE2D4 ube2d4 (Catalog #AAA3009127) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UBE2D4 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's UBE2D4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the UBE2D4 ube2d4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBE2D4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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