Testing Data
(Human Anti-Rituximab Antibody bridging ELISA for Pharmacokinetic (PK) assay developmentA microtiter plate was coated overnight with Human Anti-Rituximab Antibody, clone AbD18423 (HCA186) at a concentration of 1 ug/ml. After washing and blocking with PBST+5% BSA, 10% Human Serum was added spiked with increasing concentrations of rituximab. Detection was performed using Rat Anti-Rituximab:HRP Antibody, clone MB2A4 at a concentration of 3 ug/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three independent measurements.)
Rat RITUXIMAB Monoclonal Antibody | anti-RITUXIMAB antibody
Rat Anti RITUXIMAB
Applications
ELISA, Flow Cytometry, Functional Assay
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Synonyms
RITUXIMAB; Monoclonal Antibody; Rat Anti RITUXIMAB; Rituximab antibody (MB2A4); anti-RITUXIMAB antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
MB2A4
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Form/Format
Liquid; TRIS Buffered Saline
Concentration
1.0 mg/ml (varies by lot)
Applicable Applications for anti-RITUXIMAB antibody
ELISA (EIA), Flow Cytometry (FC/FACS)
Immunogen
F(ab)2 fragment of Rituximab
Fusion Partners
Spleen cells from immunised rats were fused with cells of the NS-1 mouse myeloma cell line.
Preservative Stabilizers
Pack Size: 0.2 mg
0.09% Sodium Azide (NaN3)
Pack Size: 2 mg
0.09% Sodium Azide (NaN3)
0.09% Sodium Azide (NaN3)
Pack Size: 2 mg
0.09% Sodium Azide (NaN3)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Testing Data
(Human Anti-Rituximab Antibody bridging ELISA for Pharmacokinetic (PK) assay developmentA microtiter plate was coated overnight with Human Anti-Rituximab Antibody, clone AbD18423 (HCA186) at a concentration of 1 ug/ml. After washing and blocking with PBST+5% BSA, 10% Human Serum was added spiked with increasing concentrations of rituximab. Detection was performed using Rat Anti-Rituximab:HRP Antibody, clone MB2A4 at a concentration of 3 ug/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three independent measurements.)
Testing Data
(Human Anti-Rituximab Antibody bridging ELISA for Pharmacokinetic (PK) assay developmentA microtiter plate was coated overnight with Human Anti-Rituximab Antibody, clone AbD18423 (HCA186) at a concentration of 1 ug/ml. After washing and blocking with PBST+5% BSA, 10% Human Serum was added spiked with increasing concentrations of rituximab. Detection was performed using Rat Anti-Rituximab:HRP Antibody, clone MB2A4 at a concentration of 3 ug/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three independent measurements.)
Testing Data
(Inhibition of rituximab binding to CD20 by Human Anti-Rituximab AntibodyFigure A. PE conjugated rituximab pre-incubated for 1h with Rat Anti-Rituximab Antibody, clone MB2A4 (red) or Rat IgG2a isotype control (grey) in ratio 1:1. Figure B. PE conjugated rituximab pre-incubated for 1h with Rat Anti-Rituximab Antibody, clone MB2A4 (red) or Rat IgG2a isotype control (grey) in ratio 5:1 (Anti-Rituximab or isotype:rituximab). All experiments performed on Daudi cell line expressing CD20 and incubated for 30 min with the antibody mix.)
Testing Data
(Inhibition of rituximab binding to CD20 by Human Anti-Rituximab AntibodyFigure A. PE conjugated rituximab pre-incubated for 1h with Rat Anti-Rituximab Antibody, clone MB2A4 (red) or Rat IgG2a isotype control (grey) in ratio 1:1. Figure B. PE conjugated rituximab pre-incubated for 1h with Rat Anti-Rituximab Antibody, clone MB2A4 (red) or Rat IgG2a isotype control (grey) in ratio 5:1 (Anti-Rituximab or isotype:rituximab). All experiments performed on Daudi cell line expressing CD20 and incubated for 30 min with the antibody mix.)
Related Product Information for anti-RITUXIMAB antibody
Description: Rat anti-rituximab antibody, clone MB2A4, recognizes the monoclonal antibody rituximab, which targets CD20. This anti-rituximab antibody is highly specific and ideal for bioanalytical assay development (PK, PD, ADA assays) in preclinical R&D and clinical trials.
Summary: Rat Anti-Rituximab Antibody, clone MB2A4, is an anti-idiotypic antibody that specifically recognizes the monoclonal antibody drug rituximab. The antibody can be used to measure the levels of rituximab and biosimilar products in bioanalytical assays. Rat Anti-Rituximab Antibody, clone MB2A4, is specific for rituximab and does not recognize other CD20 antibodies. Clone MB2A4 has been used in ELISA to monitor the levels of rituximab in patient serum following therapy (Cragg et al. 2004 and Hampson et al. 2010).
Clone MB2A4 has been used to detect rituximab bound to the surface of the Raji B cell line, however detection of rituximab bound in vivo to B-CLL cells has not been demonstrated. It is possible that complement deposition on rituximab opsonised cells inhibits binding of the Anti-Rituximab Antibody to cell bound rituximab (Beum et al. 2004). Inhibition experiments carried out with Daudi cells demonstrated that this antibody is inhibitory at a ratio of 5: 1 antibody: rituximab, but does not inhibit rituximab binding to CD20 at a ratio of 1: 1.
Rituximab (reference product branded as Rituxan) is a chimeric mouse/human monoclonal antibody approved for the treatment of certain autoimmune diseases and cancer, including non-Hodgkin's lymphoma, chronic lymphocytic leukemia and rheumatoid arthritis. The antibody is specific for the cell surface protein CD20, which is widely expressed on B cells. Through three different mechanisms of action it eliminates B cells from the body, enabling the development of a new population of healthy B cells.
Summary: Rat Anti-Rituximab Antibody, clone MB2A4, is an anti-idiotypic antibody that specifically recognizes the monoclonal antibody drug rituximab. The antibody can be used to measure the levels of rituximab and biosimilar products in bioanalytical assays. Rat Anti-Rituximab Antibody, clone MB2A4, is specific for rituximab and does not recognize other CD20 antibodies. Clone MB2A4 has been used in ELISA to monitor the levels of rituximab in patient serum following therapy (Cragg et al. 2004 and Hampson et al. 2010).
Clone MB2A4 has been used to detect rituximab bound to the surface of the Raji B cell line, however detection of rituximab bound in vivo to B-CLL cells has not been demonstrated. It is possible that complement deposition on rituximab opsonised cells inhibits binding of the Anti-Rituximab Antibody to cell bound rituximab (Beum et al. 2004). Inhibition experiments carried out with Daudi cells demonstrated that this antibody is inhibitory at a ratio of 5: 1 antibody: rituximab, but does not inhibit rituximab binding to CD20 at a ratio of 1: 1.
Rituximab (reference product branded as Rituxan) is a chimeric mouse/human monoclonal antibody approved for the treatment of certain autoimmune diseases and cancer, including non-Hodgkin's lymphoma, chronic lymphocytic leukemia and rheumatoid arthritis. The antibody is specific for the cell surface protein CD20, which is widely expressed on B cells. Through three different mechanisms of action it eliminates B cells from the body, enabling the development of a new population of healthy B cells.
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Product Notes
The RITUXIMAB (Catalog #AAA225652) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's RITUXIMAB can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the RITUXIMAB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RITUXIMAB, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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