Testing Data
(Natalizumab bridging ELISA for pharmacokinetic (PK) assay developmentA microtiter plate was coated overnight with Human Anti-Natalizumab Antibody (clone AbD21375, HCA248) at a concentration of 1 mug/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of natalizumab (therapeutic reference product) or Human Anti-Integrin Alpha 4 Antibody, MCA6104 (natalizumab biosimilar, research grade). Detection was performed using HRP conjugated Human Anti-Natalizumab Antibody (clone AbD21375_hIgG1, HCA249P) at a concentration of 2 mug/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.)
Human anti-Human Integrin alpha 4 Monoclonal Antibody | anti-itga4.S antibody
Human Anti Integrin alpha 4 (Natalizumab Biosimilar)
Gene Names
itga4.S; inta4; itga4
Reactivity
Human
Applications
ELISA
Purity
>98% by SDS PAGEPurified IgG prepared by affinity chromatography on Protein A
Synonyms
Integrin alpha 4; Monoclonal Antibody; Human Anti Integrin alpha 4 (Natalizumab Biosimilar); Integrin Alpha 4 antibody; anti-itga4.S antibody
Host
Human
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG4
Purity/Purification
>98% by SDS PAGE
Purified IgG prepared by affinity chromatography on Protein A
Purified IgG prepared by affinity chromatography on Protein A
Form/Format
Liquid; Phosphate Buffered Saline
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
1032
Applicable Applications for anti-itga4.S antibody
ELISA (EIA)
Immunogen
RAMOS cell line
Preservative Stabilizers
0.02% Proclin 300
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Testing Data
(Natalizumab bridging ELISA for pharmacokinetic (PK) assay developmentA microtiter plate was coated overnight with Human Anti-Natalizumab Antibody (clone AbD21375, HCA248) at a concentration of 1 mug/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of natalizumab (therapeutic reference product) or Human Anti-Integrin Alpha 4 Antibody, MCA6104 (natalizumab biosimilar, research grade). Detection was performed using HRP conjugated Human Anti-Natalizumab Antibody (clone AbD21375_hIgG1, HCA249P) at a concentration of 2 mug/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.)
Testing Data
(Natalizumab bridging ELISA for pharmacokinetic (PK) assay developmentA microtiter plate was coated overnight with Human Anti-Natalizumab Antibody (clone AbD21375, HCA248) at a concentration of 1 mug/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of natalizumab (therapeutic reference product) or Human Anti-Integrin Alpha 4 Antibody, MCA6104 (natalizumab biosimilar, research grade). Detection was performed using HRP conjugated Human Anti-Natalizumab Antibody (clone AbD21375_hIgG1, HCA249P) at a concentration of 2 mug/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.)
Testing Data
(Purity assessment by SDS-PAGE0.5 mug of Human Anti-Integrin Alpha 4 Antibody, MCA6104 (natalizumab biosimilar, research grade) and natalizumab (therapeutic reference product) were denatured and analyzed under reducing (R) and non-reducing (NR) conditions. Bands were visualized using Coomassie staining. Full IgG (H2L2), heavy (H) and light chains (L) are indicated.)
Testing Data
(Purity assessment by SDS-PAGE0.5 mug of Human Anti-Integrin Alpha 4 Antibody, MCA6104 (natalizumab biosimilar, research grade) and natalizumab (therapeutic reference product) were denatured and analyzed under reducing (R) and non-reducing (NR) conditions. Bands were visualized using Coomassie staining. Full IgG (H2L2), heavy (H) and light chains (L) are indicated.)
Testing Data
(Monodispersity assessment by HPLC-SECSize exclusion chromatography (SEC) of Human Anti-Integrin Alpha 4 Antibody, MCA6104 (natalizumab biosimilar, research grade) and natalizumab (therapeutic reference product) was performed on an analytical HPLC instrument. Shown are monomer peaks (H2L2), calculated relative monomer portion and aggregates. Column: MAbPac SEC-1, 5 mum; dimension: 4.0 x 150 mm; mobile phase: 50 mM sodium phosphate, pH 6.8, 300 mM NaCl, 200 mM Arginine; flow rate: 200 mul/min; inj. volume [mul]: 3; temp.: 25 degree C; detection: 280 nm; sample: mAb (1 mg/ml).)
Testing Data
(Monodispersity assessment by HPLC-SECSize exclusion chromatography (SEC) of Human Anti-Integrin Alpha 4 Antibody, MCA6104 (natalizumab biosimilar, research grade) and natalizumab (therapeutic reference product) was performed on an analytical HPLC instrument. Shown are monomer peaks (H2L2), calculated relative monomer portion and aggregates. Column: MAbPac SEC-1, 5 mum; dimension: 4.0 x 150 mm; mobile phase: 50 mM sodium phosphate, pH 6.8, 300 mM NaCl, 200 mM Arginine; flow rate: 200 mul/min; inj. volume [mul]: 3; temp.: 25 degree C; detection: 280 nm; sample: mAb (1 mg/ml).)
Related Product Information for anti-itga4.S antibody
Description: Anti-Integrin Alpha 4 Antibody is a non-therapeutic biosimilar of the monoclonal antibody drug natalizumab (Tysabri) for research use. It can be used in bioanalytical assays and for studying biological pathways affected by the drug.
Summary: Human Anti-Integrin Alpha 4 Antibody is a research grade biosimilar of the monoclonal antibody drug natalizumab. It is a recombinant human IgG4 kappa antibody with variable regions from the therapeutic antibody natalizumab.
Human Anti-Integrin Alpha 4 Antibody is for research use only. It is suitable as a reference standard in a pharmacokinetic (PK) bridging ELISA with anti-natalizumab antibodies, for example HCA248 and HCA249P. It can also be used as capture and detection reagent in an anti-drug antibody (ADA) bridging ELISA, with an anti-natalizumab antibody in IgG1 format as reference standard, for example HCA249.
Natalizumab (Tysabri) is a humanized IgG4/kappa monoclonal antibody approved as a monotherapy for the treatment of relapsing forms of multiple sclerosis and for the treatment of Crohn's disease. Natalizumab is also being evaluated for potential clinical use in the treatment of B-cell malignancies. This therapeutic antibody, directed against alpha-4 integrin (CD49d), binds to alpha-4/beta-1 integrin on the surface of circulating leucocytes. It is believed to act by blocking the interaction of alpha-4/beta-1 integrin with vascular cell adhesion molecule-1 (VCAM-1), preventing the adherence and transport of inflammatory immune cells across the intestinal lining and blood-brain barrier.
Summary: Human Anti-Integrin Alpha 4 Antibody is a research grade biosimilar of the monoclonal antibody drug natalizumab. It is a recombinant human IgG4 kappa antibody with variable regions from the therapeutic antibody natalizumab.
Human Anti-Integrin Alpha 4 Antibody is for research use only. It is suitable as a reference standard in a pharmacokinetic (PK) bridging ELISA with anti-natalizumab antibodies, for example HCA248 and HCA249P. It can also be used as capture and detection reagent in an anti-drug antibody (ADA) bridging ELISA, with an anti-natalizumab antibody in IgG1 format as reference standard, for example HCA249.
Natalizumab (Tysabri) is a humanized IgG4/kappa monoclonal antibody approved as a monotherapy for the treatment of relapsing forms of multiple sclerosis and for the treatment of Crohn's disease. Natalizumab is also being evaluated for potential clinical use in the treatment of B-cell malignancies. This therapeutic antibody, directed against alpha-4 integrin (CD49d), binds to alpha-4/beta-1 integrin on the surface of circulating leucocytes. It is believed to act by blocking the interaction of alpha-4/beta-1 integrin with vascular cell adhesion molecule-1 (VCAM-1), preventing the adherence and transport of inflammatory immune cells across the intestinal lining and blood-brain barrier.
NCBI and Uniprot Product Information
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Product Notes
The itga4.S (Catalog #AAA225705) is an Antibody produced from Human and is intended for research purposes only. The product is available for immediate purchase. The Human Anti Integrin alpha 4 (Natalizumab Biosimilar) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Integrin alpha 4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the itga4.S for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Integrin alpha 4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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