Testing Data
(Figure A. Alexa Fluor 647 conjugated Rat anti Dog CD8 and RPE conjugated Rat IgG2a isotype control . Figure B. Alexa Fluor 647 conjugated Rat anti Dog CD8 and RPE conjugated Rat anti Dog CD4 . All experiments performed on red cell lysed canine blood gated on lymphoid cells in the presence of 10% dog serum. Data acquired on the ZE5 Cell Analyzer.)
Rat anti-Dog CD4 Monoclonal Antibody | anti-CD4 antibody
RAT ANTI DOG CD4: RPE-Cy7
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
FC/FACS: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
1% Bovine Serum Albumin
5% Sucrose
After reconstitution store at 4 degree C.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
Testing Data
(Figure A. Alexa Fluor 647 conjugated Rat anti Dog CD8 and RPE conjugated Rat IgG2a isotype control . Figure B. Alexa Fluor 647 conjugated Rat anti Dog CD8 and RPE conjugated Rat anti Dog CD4 . All experiments performed on red cell lysed canine blood gated on lymphoid cells in the presence of 10% dog serum. Data acquired on the ZE5 Cell Analyzer.)
Testing Data
(Figure A. Alexa Fluor 647 conjugated Rat anti Dog CD8 and RPE conjugated Rat IgG2a isotype control . Figure B. Alexa Fluor 647 conjugated Rat anti Dog CD8 and RPE conjugated Rat anti Dog CD4 . All experiments performed on red cell lysed canine blood gated on lymphoid cells in the presence of 10% dog serum. Data acquired on the ZE5 Cell Analyzer.)
Testing Data
(Figure A. Alexa Fluor 647 conjugated Rat anti Canine CD5 and Alexa Fluor 488 conjugated Rat IgG2a isotype control . Figure B. Alexa Fluor 647 conjugated Mouse anti Canine CD5 and Alexa Fluor 488 conjugated Rat anti Canine CD4 . All experiments performed on red cell lysed canine blood gated on lymphocytes in the presence of 10% canine serum. Data acquired on the ZE5 Cell Analyzer.)
Testing Data
(Figure A. Alexa Fluor 647 conjugated Rat anti Canine CD5 and Alexa Fluor 488 conjugated Rat IgG2a isotype control . Figure B. Alexa Fluor 647 conjugated Mouse anti Canine CD5 and Alexa Fluor 488 conjugated Rat anti Canine CD4 . All experiments performed on red cell lysed canine blood gated on lymphocytes in the presence of 10% canine serum. Data acquired on the ZE5 Cell Analyzer.)
Testing Data
(Figure A. FITC conjugated Mouse anti Canine CD3 and RPE conjugated Rat IgG2a isotype control . Figure B. FITC conjugated Mouse anti Canine CD3 and RPE conjugated Rat anti Canine CD4 . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)
Testing Data
(Figure A. FITC conjugated Mouse anti Canine CD3 and RPE conjugated Rat IgG2a isotype control . Figure B. FITC conjugated Mouse anti Canine CD3 and RPE conjugated Rat anti Canine CD4 . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)
Testing Data
(Figure A. RPE conjugated Rat anti Canine CD4 and FITC conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated Rat anti Canine CD4 and FITC conjugated Mouse anti Canine CD3 . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)
Testing Data
(Figure A. RPE conjugated Rat anti Canine CD4 and FITC conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated Rat anti Canine CD4 and FITC conjugated Mouse anti Canine CD3 . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)
Testing Data
(Figure A. A647 conjugated Rat anti Canine CD4 and RPE conjugated Rat IgG2a isotype control . Figure B. A647 conjugated Rat anti Canine CD4 and RPE conjugated Rat anti Canine CD5 . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)
Testing Data
(Figure A. A647 conjugated Rat anti Canine CD4 and RPE conjugated Rat IgG2a isotype control . Figure B. A647 conjugated Rat anti Canine CD4 and RPE conjugated Rat anti Canine CD5 . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)
Testing Data
(Figure A. RPE conjugated Mouse anti Canine CD5 and A647 conjugated Rat IgG2a isotype control . Figure B. RPE conjugated Rat anti Canine CD5 and A647 conjugated Rat anti Canine CD4 . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)
Testing Data
(Figure A. RPE conjugated Mouse anti Canine CD5 and A647 conjugated Rat IgG2a isotype control . Figure B. RPE conjugated Rat anti Canine CD5 and A647 conjugated Rat anti Canine CD4 . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)
Tesing Data
(Figure A. A488 conjugated Rat anti Canine CD4 and purified Mouse IgG1 isotype control detected with Goat anti Mouse IgG1 DyLight 649 . Figure B. A488 conjugated Rat anti Canine CD4 and purified Mouse anti Canine CD8b detected with Goat anti Mouse IgG1 DyLight 649 . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)
Tesing Data
(Figure A. A488 conjugated Rat anti Canine CD4 and purified Mouse IgG1 isotype control detected with Goat anti Mouse IgG1 DyLight 649 . Figure B. A488 conjugated Rat anti Canine CD4 and purified Mouse anti Canine CD8b detected with Goat anti Mouse IgG1 DyLight 649 . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)
Tesing Data
(Figure A. Purified Mouse anti Canine CD8b detected with Goat anti Mouse IgG1 DyLight 649 and Rat IgG2b FITC isotype control . Figure B. Purified Mouse anti Canine CD8b detected with Goat anti Mouse IgG1 PE and Rat anti Canine CD4 FITC . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)
Tesing Data
(Figure A. Purified Mouse anti Canine CD8b detected with Goat anti Mouse IgG1 DyLight 649 and Rat IgG2b FITC isotype control . Figure B. Purified Mouse anti Canine CD8b detected with Goat anti Mouse IgG1 PE and Rat anti Canine CD4 FITC . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)
Tesing Data
(Published customer image:Pacific Blue conjugated Mouse anti Canine CD4 antibody, clone YKIX302.9 used for evaluation of CD4 expression on canine cells by flow cytometry.Image caption:CD11b+CD14-MHCII- cells suppress T cell proliferation. Facs sorted CD11b+CD14-MHCII- cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4+ and CD8+ T cells isolated from a healthy dog for 72 hrs. No stimulated cells were used as negative control. Proliferative responses were measured by 3H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean +/- SEM are shown.From: Goulart MR, Pluhar GE, Ohlfest JR (2012)Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer.PLoS ONE 7(3): e33274.)
Tesing Data
(Published customer image:Pacific Blue conjugated Mouse anti Canine CD4 antibody, clone YKIX302.9 used for evaluation of CD4 expression on canine cells by flow cytometry.Image caption:CD11b+CD14-MHCII- cells suppress T cell proliferation. Facs sorted CD11b+CD14-MHCII- cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4+ and CD8+ T cells isolated from a healthy dog for 72 hrs. No stimulated cells were used as negative control. Proliferative responses were measured by 3H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean +/- SEM are shown.From: Goulart MR, Pluhar GE, Ohlfest JR (2012)Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer.PLoS ONE 7(3): e33274.)
Tesing Data
(Published customer image:RPE conjugated Mouse anti Canine CD4 antibody, clone YKIC302.9used for the assessment of CD4 levels on canine cells by flow cytometry.Image caption:Immunophenotypic profile of tumor infiltrating lymphocyte in canine mammary carcinomas. Analysis of tumor infiltrating T-cells, B-lymphocytes and T-cell subsets from MC-BMT or MC (A), further subcategorized according to the absence (-) or presence (+) of lymph node metastasis (-) (B). Lymphocyte populations and subsets were identified by flow cytometric immunostaining as described in Material and Methods. Data were expressed as percentage of positive cells within gated lymphocytes and CD4+/CD8+ T-cell ratio. Significant differences at p < 0.05 are highlighted by asterisk.From: From: Estrela-Lima et al.BMC Cancer 2010 10:256.)
Tesing Data
(Published customer image:RPE conjugated Mouse anti Canine CD4 antibody, clone YKIC302.9used for the assessment of CD4 levels on canine cells by flow cytometry.Image caption:Immunophenotypic profile of tumor infiltrating lymphocyte in canine mammary carcinomas. Analysis of tumor infiltrating T-cells, B-lymphocytes and T-cell subsets from MC-BMT or MC (A), further subcategorized according to the absence (-) or presence (+) of lymph node metastasis (-) (B). Lymphocyte populations and subsets were identified by flow cytometric immunostaining as described in Material and Methods. Data were expressed as percentage of positive cells within gated lymphocytes and CD4+/CD8+ T-cell ratio. Significant differences at p < 0.05 are highlighted by asterisk.From: From: Estrela-Lima et al.BMC Cancer 2010 10:256.)
Rat anti Dog CD4 antibody, clone YKIX302.9 partially depletes circulating T lymphocytes when administered in vivo, but alone is not sufficient to prolong allograft survival in a canine transplant model (Watson et al. 1993).
Uniquely amongst mammals, canine CD4 is expressed by neutrophils as well as by lymphocyte subsets (Moore et al. 1992).
2. Schaut, R.G. et al. (2016) Recovery of antigen-specific T cell responses from dogs infected with Leishmania (L.) infantum by use of vaccine associated TLR-agonist adjuvant.Vaccine. 34 (44): 5225-34.
3. Gorman, S.D. et al. (1994) Isolation and expression of cDNA encoding the canine CD4 and CD8 alpha antigens.Tissue Antigens. 43 (3): 184-8.
4. Watson, C.J. et al. (1993) CD4 and CD8 monoclonal antibody therapy: strategies to prolong renal allograft survival in the dog.Br J Surg. 80 (11): 1389-92.
5. Papadogiannakis, E.I. et al. (2009) Determination of intracellular cytokines IFN-gamma and IL-4 in canine T lymphocytes by flow cytometry following whole-blood culture.Can J Vet Res. 73 (2): 137-43.
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Product Notes
The CD4 (Catalog #AAA225940) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The RAT ANTI DOG CD4: RPE-Cy7 reacts with Dog and may cross-react with other species as described in the data sheet. AAA Biotech's CD4 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: Max Dilution: Neat FC/FACS: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Researchers should empirically determine the suitability of the CD4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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