Testing Data
(Figure A. RPE conjugated Mouse anti Chicken CD8 and FITC conjugated Mouse IgG2b isotype control . Figure B. PE conjugated Mouse anti Chicken CD8 and FITC conjugated Mouse anti Chicken CD4 . All experiments performed on peripheral blood mononuclear cells extracted from whole chicken blood gated on lymphocytes. Data acquired on the ZE5 Cell Analyzer.)
Mouse CD4 Monoclonal Antibody | anti-CD4 antibody
Mouse Anti Chicken CD4
Reactivity
Chicken, Turkey
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Synonyms
CD4; Monoclonal Antibody; Mouse Anti Chicken CD4; CD4 antibody (2-35); anti-CD4 antibody
Host
Mouse
Reactivity
Chicken, Turkey
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
2-35
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Form/Format
Liquid; Phosphate Buffered Saline
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
487
Applicable Applications for anti-CD4 antibody
Immunohistochemistry (IHC) Frozen, Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
Immunogen
Chicken embryonic thymocytes
Carrier Free
Yes
Fusion Partners
Lymph node cells from immunised Balb/c mice were fused with cells of the SP2/0 myeloma cell line.
Preservative Stabilizers
0.09% Sodium Azide (NaN3)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Testing Data
(Figure A. RPE conjugated Mouse anti Chicken CD8 and FITC conjugated Mouse IgG2b isotype control . Figure B. PE conjugated Mouse anti Chicken CD8 and FITC conjugated Mouse anti Chicken CD4 . All experiments performed on peripheral blood mononuclear cells extracted from whole chicken blood gated on lymphocytes. Data acquired on the ZE5 Cell Analyzer.)
Testing Data
(Figure A. RPE conjugated Mouse anti Chicken CD8 and FITC conjugated Mouse IgG2b isotype control . Figure B. PE conjugated Mouse anti Chicken CD8 and FITC conjugated Mouse anti Chicken CD4 . All experiments performed on peripheral blood mononuclear cells extracted from whole chicken blood gated on lymphocytes. Data acquired on the ZE5 Cell Analyzer.)
Testing Data
(Figure A. FITC conjugated Mouse anti Chicken CD8 and RPE conjugated Mouse IgG2b isotype control . Figure B. FITC conjugated Mouse anti Chicken CD8 and RPE conjugated Mouse anti Chicken CD4 . All experiments performed on peripheral blood mononuclear cells extracted from whole chicken blood gated on lymphocytes. Data acquired on the ZE5 Cell Analyzer.)
Testing Data
(Figure A. FITC conjugated Mouse anti Chicken CD8 and RPE conjugated Mouse IgG2b isotype control . Figure B. FITC conjugated Mouse anti Chicken CD8 and RPE conjugated Mouse anti Chicken CD4 . All experiments performed on peripheral blood mononuclear cells extracted from whole chicken blood gated on lymphocytes. Data acquired on the ZE5 Cell Analyzer.)
Testing Data
(Figure A. FITC conjugated Mouse anti Chicken CD8 and Mouse IgG2b isotype control conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE . Figure B. Mouse anti Chicken CD4 conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE and FITC conjugated Mouse anti Chicken CD8 . All experiments performed on peripheral blood mononuclear cells extracted from whole chicken blood gated on lymphocytes. Data acquired on the ZE5 Cell Analyzer.)
Testing Data
(Figure A. FITC conjugated Mouse anti Chicken CD8 and Mouse IgG2b isotype control conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE . Figure B. Mouse anti Chicken CD4 conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE and FITC conjugated Mouse anti Chicken CD8 . All experiments performed on peripheral blood mononuclear cells extracted from whole chicken blood gated on lymphocytes. Data acquired on the ZE5 Cell Analyzer.)
Related Product Information for anti-CD4 antibody
Mouse anti Chicken CD4, clone 2-35 recognizes the chicken homologue of human CD4, a ~64 kDa cell surface protein expressed by thymocytes and a subset of T cells (Luhtala et al. 1993). Mouse anti Chicken CD4, clone 2-35 has been demonstrated to recognize turkey CD4 (Li et al. 1998).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
CD4
NCBI Official Synonym Full Names
CD4 molecule
NCBI Official Symbol
CD4
NCBI Protein Information
T-cell surface glycoprotein CD4
UniProt Protein Name
CD4
Protein Family
UniProt Gene Name
CD4
Research Articles on CD4
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Product Notes
The CD4 cd4 (Catalog #AAA225645) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti Chicken CD4 reacts with Chicken, Turkey and may cross-react with other species as described in the data sheet. AAA Biotech's CD4 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Frozen, Flow Cytometry (FC/FACS), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the CD4 cd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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