Testing Data
(0.5g of Human Anti-CD20 Antibody, (rituximab biosimilar, research grade) and rituximab (therapeutic reference product) were denatured and analyzed under reducing (R) and non-reducing (NR) conditions. Bands were visualized using Coomassie staining. Full IgG (H2L2), heavy (H) and light chains (L) are indicated.)
Human anti-Human CD20 Monoclonal Antibody | anti-CD20 antibody
HUMAN ANTI CD20 (RITUXIMAB BIOSIMILAR)
Reactivity
Human
Applications
ELISA
Purity
>98% by SDS PAGE
Synonyms
CD20; Monoclonal Antibody; HUMAN ANTI CD20 (RITUXIMAB BIOSIMILAR); CD20 antibody|10F381; anti-CD20 antibody
Host
Human
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
10F381
Specificity
CD20
Purity/Purification
>98% by SDS PAGE
Form/Format
Human IgG1 (kappa) antibody, liquid. Phosphate buffered saline
Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line
Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Applicable Applications for anti-CD20 antibody
ELISA (EIA)
Application Notes
Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Immunogen
Human lymphoblastoid cell line SB
Preservative Stabilizers
0.02% Proclin 300
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Testing Data
(0.5g of Human Anti-CD20 Antibody, (rituximab biosimilar, research grade) and rituximab (therapeutic reference product) were denatured and analyzed under reducing (R) and non-reducing (NR) conditions. Bands were visualized using Coomassie staining. Full IgG (H2L2), heavy (H) and light chains (L) are indicated.)
Testing Data
(0.5g of Human Anti-CD20 Antibody, (rituximab biosimilar, research grade) and rituximab (therapeutic reference product) were denatured and analyzed under reducing (R) and non-reducing (NR) conditions. Bands were visualized using Coomassie staining. Full IgG (H2L2), heavy (H) and light chains (L) are indicated.)
Related Product Information for anti-CD20 antibody
Human Anti-CD20 antibody, clone 10F381 is a research grade biosimilar of the monoclonal antibody drug rituximab. It is a recombinant human IgG1 kappa antibody with variable regions from the therapeutic rituximab antibody clone 10F381.
This antibody is for research use only. It is suitable as a reference standard in a pharmacokinetic (PK) bridging ELISA with anti-rituximab antibodies, for example HCA186 and HCA201. It can also be used as capture and detection reagent in an anti-drug antibody (ADA) bridging ELISA, with an anti-rituximab antibody in IgG1 format as a reference standard, for example HCA201.
Rituximab is a chimeric monoclonal antibody with murine variable region and human constant domains. It targets CD20, a protein found on the surface of B cells. Binding of the drug to its target triggers cell death through a number of mechanisms, including apoptosis, complement activation and cell-mediated cytotoxicity. Rituximab has been approved for the treatment of B cell malignancies, transplant rejection and autoimmune disorders.
Anti-CD20 Antibody is a non-therapeutic biosimilar of the monoclonal antibody drug rituximab (Rituxan) for research use. It can be used in bioanalytical PK and ADA assays and for studying biological pathways affected by the drug.
This antibody is for research use only. It is suitable as a reference standard in a pharmacokinetic (PK) bridging ELISA with anti-rituximab antibodies, for example HCA186 and HCA201. It can also be used as capture and detection reagent in an anti-drug antibody (ADA) bridging ELISA, with an anti-rituximab antibody in IgG1 format as a reference standard, for example HCA201.
Rituximab is a chimeric monoclonal antibody with murine variable region and human constant domains. It targets CD20, a protein found on the surface of B cells. Binding of the drug to its target triggers cell death through a number of mechanisms, including apoptosis, complement activation and cell-mediated cytotoxicity. Rituximab has been approved for the treatment of B cell malignancies, transplant rejection and autoimmune disorders.
Anti-CD20 Antibody is a non-therapeutic biosimilar of the monoclonal antibody drug rituximab (Rituxan) for research use. It can be used in bioanalytical PK and ADA assays and for studying biological pathways affected by the drug.
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Product Notes
The CD20 (Catalog #AAA226035) is an Antibody produced from Human and is intended for research purposes only. The product is available for immediate purchase. The HUMAN ANTI CD20 (RITUXIMAB BIOSIMILAR) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD20 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Researchers should empirically determine the suitability of the CD20 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD20, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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