Testing Data
(Figure A. RPE conjugated rat anti mouse TER-119 and FITC conjugated Rat IgG2a isotype control Figure B. RPE conjugated rat anti mouse TER-119 and FITC conjugated rat anti mouse CD71 . All experiments performed on mouse bone marrow. Data acquired on the ZE5 Cell Analyzer)
Rat anti-Mouse CD71 Monoclonal Antibody | anti-CD71 antibody
Rat Anti Mouse CD71: RPE
Gene Names
Tfrc; TR; TFR; p90; CD71; TFR1; Trfr; Mtvr1; 2610028K12Rik; E430033M20Rik
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Synonyms
CD71; Monoclonal Antibody; Rat Anti Mouse CD71: RPE; CD71 antibody (8D3); anti-CD71 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
8D3
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Form/Format
Liquid; Phosphate Buffered Saline
Concentration
0.1 mg/ml (varies by lot)
Sequence Length
763
Applicable Applications for anti-CD71 antibody
Flow Cytometry (FC/FACS)
Immunogen
Mouse transformed endothelioma cell line t.end1.
Conjugation
RPE
Fusion Partners
Spleen cells from immunised Lewis rats were fused with the cells of the mouse NS0 myeloma cell line.
Preservative Stabilizers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
1% Bovine Serum Albumin
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Testing Data
(Figure A. RPE conjugated rat anti mouse TER-119 and FITC conjugated Rat IgG2a isotype control Figure B. RPE conjugated rat anti mouse TER-119 and FITC conjugated rat anti mouse CD71 . All experiments performed on mouse bone marrow. Data acquired on the ZE5 Cell Analyzer)
Testing Data
(Figure A. RPE conjugated rat anti mouse TER-119 and FITC conjugated Rat IgG2a isotype control Figure B. RPE conjugated rat anti mouse TER-119 and FITC conjugated rat anti mouse CD71 . All experiments performed on mouse bone marrow. Data acquired on the ZE5 Cell Analyzer)
Testing Data
(Figure A. FITC conjugated rat anti mouse TER-119 and RPE conjugated Rat IgG2a isotype control Figure B. FITC conjugated rat anti mouse TER-119 and RPE conjugated rat anti mouse CD71 . All experiments performed on mouse bone marrow. Data acquired on the ZE5 Cell Analyzer.)
Testing Data
(Figure A. FITC conjugated rat anti mouse TER-119 and RPE conjugated Rat IgG2a isotype control Figure B. FITC conjugated rat anti mouse TER-119 and RPE conjugated rat anti mouse CD71 . All experiments performed on mouse bone marrow. Data acquired on the ZE5 Cell Analyzer.)
Related Product Information for anti-CD71 antibody
Rat anti Mouse CD71 antibody, clone 8D3 recognizes mouse CD71, a 763 amino acid ~95 kDa single pass type II cell surface transmembrane glycoprotein, otherwise known as the transferrin receptor. CD71 is a major iron-binding protein, which plays a key role in the transport of iron into cells that require it. In mice, CD71 is widely expressed on a variety of cells, including Sertoli cells and cells which form the blood brain barrier (BBB) in the central nervous system.
Rat anti Mouse CD71 antibody, clone 8D3 recognizes native, soluble and denatured forms of murine CD71. Binding of the 8D3 antibody to CD71 does not inhibit the proliferation of cell lines tested, and does not interfere with the uptake of iron into cells.
Rat anti Mouse CD71 antibody, clone 8D3 has been used as a BBB transporter vector in mice and is suitable for studying CD71 expression, and iron uptake into different tissues, in the mouse (Kissel et al. 1988).
Rat anti Mouse CD71 antibody, clone 8D3 recognizes native, soluble and denatured forms of murine CD71. Binding of the 8D3 antibody to CD71 does not inhibit the proliferation of cell lines tested, and does not interfere with the uptake of iron into cells.
Rat anti Mouse CD71 antibody, clone 8D3 has been used as a BBB transporter vector in mice and is suitable for studying CD71 expression, and iron uptake into different tissues, in the mouse (Kissel et al. 1988).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
transferrin receptor protein 1
NCBI Official Synonym Full Names
transferrin receptor
NCBI Official Symbol
Tfrc
NCBI Official Synonym Symbols
TR; TFR; p90; CD71; TFR1; Trfr; Mtvr1; 2610028K12Rik; E430033M20Rik
NCBI Protein Information
transferrin receptor protein 1
UniProt Protein Name
Transferrin receptor protein 1
UniProt Entry Name
TFR1_MOUSE
NCBI Description
This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Mice that are deficient in this receptor show impaired erythroid development and abnormal iron homeostasis. [provided by RefSeq, Sep 2015]
Research Articles on CD71
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Product Notes
The CD71 (Catalog #AAA225662) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti Mouse CD71: RPE reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD71 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the CD71 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD71, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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