Testing Data
(Detection of Rituximab spiked into 10% human serum by sandwich ELISA. Human anti Rituximab (HCA186) was used as the coating antibody and detection was performed by adding HRP conjugated anti-Rituximab antibody MBS215727. Data are shown as the mean of three independent measurements)
Rat RITUXIMAB Monoclonal Antibody
RAT ANTI RITUXIMAB:FITC
Applications
Flow Cytometry, Functional Assay
Synonyms
RITUXIMAB; Monoclonal Antibody; RAT ANTI RITUXIMAB:FITC; anti-RITUXIMAB antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
MB2A4
Form/Format
FITC
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 0.1 mg/ml (varies by lot)
Applicable Applications for anti-RITUXIMAB antibody
Flow cytometry (FC/FACS)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Maximum Dilution: Neat
Flow Cytometry: Maximum Dilution: Neat
Perservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Preparation
1% Bovine Serum Albumin
Preparation
Buffer Solution
Phosphate buffered saline
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Testing Data
(Detection of Rituximab spiked into 10% human serum by sandwich ELISA. Human anti Rituximab (HCA186) was used as the coating antibody and detection was performed by adding HRP conjugated anti-Rituximab antibody MBS215727. Data are shown as the mean of three independent measurements)
Testing Data
(Detection of Rituximab spiked into 10% human serum by sandwich ELISA. Human anti Rituximab (HCA186) was used as the coating antibody and detection was performed by adding HRP conjugated anti-Rituximab antibody MBS215727. Data are shown as the mean of three independent measurements)
Testing Data
(Detection of Rituximab in direct ELISA. Rituximab was used as the coating reagent followed by Rat anti Rituximab:HRP (MBS224427) as the detection antibody)
Testing Data
(Detection of Rituximab in direct ELISA. Rituximab was used as the coating reagent followed by Rat anti Rituximab:HRP (MBS224427) as the detection antibody)
Related Product Information for anti-RITUXIMAB antibody
Rat anti rituximab antibody, clone MB2A4, specifically recognizes the idiotypic determinants expressed by the rituximab chimeric monoclonal antibody.Rituximab is a therapeutic reagent directed against the human CD20 antigen expressed on the surface of B-cells. Rat anti rituximab antibody, clone MB2A4, is specific for rituximab and does not recognize other CD20 antibodies. Clone MB2A4 has been used in ELISA assays to monitor the levels of rituximab in patient serum following therapy, see Cragg, M. S. et al. and Hampson, G. et al. for details. Clone MB2A4 has been used to detect rituximab bound to the surface of the Raji B cell line, however detection of rituximab bound in vivo to B-CLL cells has not been demonstrated. It is possible that complement deposition on rituximab opsonised cells inhibits binding of the anti-rituximab antibody to cell bound rituximab (Beum, P. V. et al.).
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Product Notes
The RITUXIMAB (Catalog #AAA215728) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's RITUXIMAB can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Maximum Dilution: Neat. Researchers should empirically determine the suitability of the RITUXIMAB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RITUXIMAB, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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