Testing Data
(Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD19 and Amethyst Orange conjugated Mouse IgG2b isotype control . Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD19 and Amethyst Orange conjugated Mouse anti Human CD20 . All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
Mouse anti-Human CD20 Monoclonal Antibody | anti-CD20 antibody
Mouse Anti Human CD20: Amethyst Orange
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody.
This product contains a precipitate we recommend microcentrifugation before use.
Testing Data
(Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD19 and Amethyst Orange conjugated Mouse IgG2b isotype control . Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD19 and Amethyst Orange conjugated Mouse anti Human CD20 . All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
Testing Data
(Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD19 and Amethyst Orange conjugated Mouse IgG2b isotype control . Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD19 and Amethyst Orange conjugated Mouse anti Human CD20 . All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
Testing Data
(Figure A. RPE conjugated Mouse anti Human CD19 and FITC conjugated Mouse IgG2b isotype control . Figure B. RPE conjugated Mouse anti Human CD19 and FITC conjugated Mouse anti Human CD20 . All experiments performed on red cell lysed human peripheral blood gated on lymphocytes in the presence of Human SeroBlock .)
Testing Data
(Figure A. RPE conjugated Mouse anti Human CD19 and FITC conjugated Mouse IgG2b isotype control . Figure B. RPE conjugated Mouse anti Human CD19 and FITC conjugated Mouse anti Human CD20 . All experiments performed on red cell lysed human peripheral blood gated on lymphocytes in the presence of Human SeroBlock .)
Testing Data
(FigureA.FITC conjugated Mouse anti Human CD19 and Alexa Fluor 647 conjugated Mouse IgG2b isotype control . Figure B. FITC conjugated Mouse anti Human CD19 and Alexa Fluor 647 conjugated Mouse anti Human CD20 . All experiments performed on human peripheral blood lymphocytes in the presence of Human SeroBlock .)
Testing Data
(FigureA.FITC conjugated Mouse anti Human CD19 and Alexa Fluor 647 conjugated Mouse IgG2b isotype control . Figure B. FITC conjugated Mouse anti Human CD19 and Alexa Fluor 647 conjugated Mouse anti Human CD20 . All experiments performed on human peripheral blood lymphocytes in the presence of Human SeroBlock .)
Testing Data
(Mouse anti Human CD20 antibody, clone 2C7 in an immunohistochemical analysis of acetone fixed, frozen human tonsil section detected using the Histar Detection Kit )
Testing Data
(Mouse anti Human CD20 antibody, clone 2C7 in an immunohistochemical analysis of acetone fixed, frozen human tonsil section detected using the Histar Detection Kit )
Testing Data
(APC Conjugated Mouse anti Human CD20 antibody, clone 2H7 used as part of a panel of antibodies to identify lymphocyte populations in feline peripheral blood by flow cytometry.Image caption:Gating strategy used in flow cytometric analyses of PBMC.Representative pseudocolor plots are shown. After exclusion of dead cells (A) and doublets (B), gating on lymphocytes and monocytes was performed according to their forward and side scattering (FSC/SSC)properties (C). (D) Among lymphocytes CD4+ T cells, CD8+ T cells and CD20+ B cells (upper panel) were gated according to the appropriate Fluorescence-minus-one (FMO) controls (lower panel).From: Sieg M, Busch J, Eschke M, Böttcher D, Heenemann K, Vahlenkamp A, Reinert A, Seeger J, Heilmann R, Scheffler K, Vahlenkamp TW.A New Genotype of Feline Morbillivirus Infects Primary Cells of the Lung, Kidney, Brain and Peripheral Blood.Viruses. 2019 Feb 9;11(2). pii: E146.doi: 10.3390/v11020146.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Testing Data
(APC Conjugated Mouse anti Human CD20 antibody, clone 2H7 used as part of a panel of antibodies to identify lymphocyte populations in feline peripheral blood by flow cytometry.Image caption:Gating strategy used in flow cytometric analyses of PBMC.Representative pseudocolor plots are shown. After exclusion of dead cells (A) and doublets (B), gating on lymphocytes and monocytes was performed according to their forward and side scattering (FSC/SSC)properties (C). (D) Among lymphocytes CD4+ T cells, CD8+ T cells and CD20+ B cells (upper panel) were gated according to the appropriate Fluorescence-minus-one (FMO) controls (lower panel).From: Sieg M, Busch J, Eschke M, Böttcher D, Heenemann K, Vahlenkamp A, Reinert A, Seeger J, Heilmann R, Scheffler K, Vahlenkamp TW.A New Genotype of Feline Morbillivirus Infects Primary Cells of the Lung, Kidney, Brain and Peripheral Blood.Viruses. 2019 Feb 9;11(2). pii: E146.doi: 10.3390/v11020146.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
The CD20 antigen is expressed during pre-B-cell development. It is present on both resting and activated B-cells but is lost prior to terminal B-cell differentiation into plasma cells.
The epitope recognized by clone 2H7 has been mapped to the following sequence found in the large extracellular loop of human CD20: YNCEPANPSEKNSPST. Furthermore it appears that Mouse anti Human CD20 antibody, clone 2H7 only recognizes human CD20 in its native oligomeric form (Polyak et al. 2002).
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008]
Uniprot Description
MS4A1: This protein may be involved in the regulation of B-cell activation and proliferation. Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5); also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low. Belongs to the MS4A family.
Protein type: Membrane protein, integral; Cell surface; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 11q12
Cellular Component: extracellular space; integral to plasma membrane; external side of plasma membrane
Molecular Function: epidermal growth factor receptor binding
Biological Process: B cell proliferation; humoral immune response
Disease: Immunodeficiency, Common Variable, 5
Research Articles on CD20
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Product Notes
The CD20 ms4a1 (Catalog #AAA225851) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti Human CD20: Amethyst Orange reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD20 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: Min. Dilution: Neat Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood. Researchers should empirically determine the suitability of the CD20 ms4a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD20, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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