Testing Data
Mouse anti-Human Nuclei Monoclonal Antibody
Anti-Nuclei
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry
Synonyms
Nuclei; Monoclonal Antibody; Anti-Nuclei; marker for human cells in xenographic research; anti-Nuclei antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Clone Number
235-1
Specificity
Specific for human nuclei. Stains nuclei of all human cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. By immunoprecipitation the antibody reacts with an 80 kDa and 70 kDa band. The antibody works on acetone fixed and paraformaldehyde fixed cells. It is suggested that you permeabilize the cells with 0.1-0.4% Triton X100. The antibody also works for immunohistochemistry on paraformaldehyde fixed cryostat sections.
Applicable Applications for anti-Nuclei antibody
Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes
Quality Control: Immunofluorescence performed on each lot.
IHC/IF: 1: 100
IHC/IF: 1: 100
Packaging
100ul culture supernantant. Adequate amount of material to conduct 10 mini Western Blots.
Preparation and Storage
Store at -20°C; stable for at least one year.
Shipping: Blue Ice
Shipping: Blue Ice
Testing Data
Testing Data
Related Product Information for anti-Nuclei antibody
Mouse monoclonal antibody
References
• Glaser R, Lu MM, Narula N, Epstein JA (2002) Smooth muscle cells, but not myocytes, of host origin in transplanted human hearts. Circulation Jul 2;106(1):17-9.
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Product Notes
The Nuclei (Catalog #AAA500034) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Nuclei reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Nuclei can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC). Quality Control: Immunofluorescence performed on each lot. IHC/IF: 1: 100. Researchers should empirically determine the suitability of the Nuclei for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nuclei, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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