Mouse Prox1 Monoclonal Antibody | anti-PROX1 antibody

Anti-Prox1

Cat. Num. AAA500038

• Reactivity: Chicken, human, mouse, rat
• Applications: Immunohistochemistry
• Purity: Protein G purified (Protein G purified culture supernatant.)
• Synonyms: Prox1; Monoclonal Antibody; Anti-Prox1; anti-PROX1 antibody

• Host: Mouse
• Reactivity: Chicken, human, mouse, rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 5G10
• Specificity: Specifically labels prox1 in formalin-fixed, paraffin-embedded tissue.
• Purity/Purification: Protein G purified (Protein G purified culture supernatant.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 737

• Applicable Applications for anti-PROX1 antibody: Immunohistochemistry (IHC)
• Application Notes: Quality Control: IHC performed on each lot.; IHC: 1:1,000
• Antigen: Recombinant human Prox1 protein.
• Immunogen Information: Recombinant human Prox1 protein
• Immunogen Species: Human
• Species Reactivity Note: The antibody has been directly tested for reactivity in human, rat, mouse and chicken. It is expected to have wide species cross reactivity.
• Biological Significance: Prox1 (Prospero-related homeobox 1) is a divergent homeogene that regulates cell proliferation, fate determination and differentiation during vertebrate embryonic development. The protein is expressed in the developing lens and is also detected in embryonic brain, lung, liver and kidney; while in adult it is more abundant in heart and liver than in brain, skeletal muscle, kidney and pancreas (Chen et al., 2006). Prox1 is thought to play a fundamental role in early development of the CNS and may regulate gene expression and development of postmitotic undifferentiated young neurons (Misra et al., 2008)
• Preparation and Storage: Store at -20 degree C in undiluted aliquots; stable for at least one year after date of receipt. Avoid freeze/thaw cycles.

Testing Data

Related Product Information for anti-PROX1 antibody

Mouse monoclonal antibody

References

• Xiaoren Chen, Tapan P. Patel, William J. Cain, Melinda K. Duncan. Production of monoclonal antibodies against Prox1. Hybridoma (2006) Feb;25(1):27-33.
• Misra K, Gui H, Matise MP (2008) Prox1 regulates a transitory state for interneuron neurogenesis in the spinal cord. Dev Dyn 237(2):393-402.

NCBI and Uniprot Product Information

• NCBI GI #: 397174762
• NCBI GeneID: 5629
• NCBI Accession #: NP_001257545.1
• NCBI GenBank Nucleotide #: NM_001270616.1
• UniProt Accession #: Q92786; Q5SW76; Q8TB91; A6NK29; A8K2B1
• Molecular Weight: 83,203 Da
• NCBI Official Full Name: prospero homeobox protein 1
• NCBI Official Synonym Full Names: prospero homeobox 1
• NCBI Official Symbol: PROX1
• NCBI Protein Information: prospero homeobox protein 1; prospero-related homeobox 1; homeobox prospero-like protein PROX1
• UniProt Protein Name: Prospero homeobox protein 1
• Protein Family: Prospero homeobox protein
• UniProt Gene Name: PROX1
• UniProt Synonym Gene Names: PROX-1
• UniProt Entry Name: PROX1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the homeobox transcription factor family. Members of this family contain a homeobox domain that consists of a 60-amino acid helix-turn-helix structure that binds DNA and RNA. The protein encoded by this gene is conserved across vertebrates and may play an essential role during development. Altered levels of this protein have been reported in cancers of different organs, such as colon, brain, blood, breast, pancreas, liver and esophagus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012]

Uniprot Description

Function: May play a fundamental role in early development of CNS. May regulate gene expression and development of postmitotic undifferentiated young neurons

By similarity.

Subcellular location: Nucleus

Probable.

Tissue specificity: Most actively expressed in the developing lens. Detected also in embryonic brain, lung, liver and kidney. In adult, it is more abundant in heart and liver than in brain, skeletal muscle, kidney and pancreas.

Sequence similarities: Belongs to the Prospero homeobox family.Contains 1 Prospero-type homeobox DNA-binding domain.

Sequence caution: The sequence CAI15309.1 differs from that shown. Reason: Erroneous gene model prediction.