Testing Data
Mouse anti-Human, rat Calcitonin Gene Related Peptide (CGRP) Monoclonal Antibody | anti-CALCA antibody
Anti-Calcitonin Gene Related Peptide (CGRP)
Gene Names
Calca; CAL6; CGRP; Cal1; Calc; RATCAL6; calcitonin
Reactivity
Human, rat
Applications
Immunocytochemistry
Purity
Protein G purified
Synonyms
Calcitonin Gene Related Peptide (CGRP); Monoclonal Antibody; Anti-Calcitonin Gene Related Peptide (CGRP); anti-CALCA antibody
Host
Mouse
Reactivity
Human, rat
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
4901
Specificity
Specific for the CGRP protein in rat DRG neurons.
Purity/Purification
Protein G purified
Form/Format
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western blots.
Sequence Length
128
Applicable Applications for anti-CALCA antibody
Immunocytochemistry (ICC)
Application Notes
Quality Control: ICC performed on each lot.
WB: not recommended
ICC: 1:50-1:100
WB: not recommended
ICC: 1:50-1:100
Antigen
Rat alpha-CGRP
Immunogen Information
Purified rat alpha-CGRP
Immunogen Species
Rat
Reactivity Assumed Based on 100% Sequence Homology
Most mammals
Species Reactivity Note
The antibody has been directly tested for reactivity in rat and human. Other species have not yet been tested. It is expected that the antibody will react with most mammals.
Biological Significance
Calcitonin Gene Related Peptide [CGRP] is a 37 amino acid peptide and is the most potent endogenous vasodilator currently known (Brain et al.,1985). CGRP is primarily produced in nervous tissue, however, its receptors are expressed throughout the body. CGRP is also strongly implicated in the vasodilatory effect of endogenous cannabinoid anandamide in the brain. This effect was found to be antagonized by capsazepine. CGRP is also currently a major target of research in regards to factors effecting the onset of migraine headaches (Edvinsson L., 2003). CGRP antibodies have been used successfully in immunoneutralization (Wong et al., 1993)
Preparation and Storage
Store at -20 degree C in undiluted aliquots; stable for at least one year after date of receipt. Avoid freeze/thaw cycles.
Testing Data
Testing Data
Related Product Information for anti-CALCA antibody
Mouse monoclonal antibody
References
• Brain SD, Williams TJ, Tippins JR, Morris HR, MacIntyre I (1985) Calcitonin gene related peptide is a potent vasodilator. Nature 313 (5997): 54-6
• Edvinsson L (2003) New therapeutic target in primary headaches—blocking the CGRP receptor. Expert Opin Ther Targets.7(3):377-83
• Wong HC, Tache' Y, Lloud KCK, Yang H, Sternini C, Holzer P, Walsh JH (1993) Monoclonal antibody to rat alpha-CGRP: production, charactrization, and in vivo immunoneutralization activity. Hybridoma. 12:93-106.
• Edvinsson L (2003) New therapeutic target in primary headaches—blocking the CGRP receptor. Expert Opin Ther Targets.7(3):377-83
• Wong HC, Tache' Y, Lloud KCK, Yang H, Sternini C, Holzer P, Walsh JH (1993) Monoclonal antibody to rat alpha-CGRP: production, charactrization, and in vivo immunoneutralization activity. Hybridoma. 12:93-106.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13,948 Da
NCBI Official Full Name
calcitonin gene-related peptide 1 isoform Cgrp preproprotein
NCBI Official Synonym Full Names
calcitonin-related polypeptide alpha
NCBI Official Symbol
Calca
NCBI Official Synonym Symbols
CAL6; CGRP; Cal1; Calc; RATCAL6; calcitonin
NCBI Protein Information
calcitonin gene-related peptide 1; CGRP-I; alpha-type CGRP; calcitonin gene-related peptide I; calcitonin/calcitonin-related polypeptide, alpha
UniProt Protein Name
Calcitonin gene-related peptide 1
Protein Family
UniProt Gene Name
Calca
UniProt Synonym Gene Names
Calc; CGRP-I
UniProt Entry Name
CALCA_RAT
NCBI Description
an inhibitor of lactotroph function and a potent vasodilator [RGD, Feb 2006]
Uniprot Description
Function: CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role.
Subcellular location: Secreted.
Sequence similarities: Belongs to the calcitonin family.
Research Articles on CALCA
Similar Products
Product Notes
The CALCA calca (Catalog #AAA500026) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Calcitonin Gene Related Peptide (CGRP) reacts with Human, rat and may cross-react with other species as described in the data sheet. AAA Biotech's Calcitonin Gene Related Peptide (CGRP) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC). Quality Control: ICC performed on each lot. WB: not recommended ICC: 1:50-1:100. Researchers should empirically determine the suitability of the CALCA calca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calcitonin Gene Related Peptide (CGRP), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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