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Testing Data (Figure 1 SARS-Cov-2 Spike P681R (Delta Variant) Antibodies Specifically Detect Delta Variant Spike S1 Protein in an ELISA Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 μg/mL, incubated at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 Spike P681R (Delta Variant) antibody, PM-9677, 1 μg/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.)

Mouse COVID 19 Spike P681R Antibody [5H4C5] (Delta Variant) Coronavirus Monoclonal Antibody | anti-COVID-19 antibody

SARS-CoV-2 (COVID-19) Spike P681R Antibody [5H4C5] (Delta Variant)

Reactivity
Virus
Applications
ELISA
Purity
Anti-SARS-CoV-2 Spike P681R (Delta Variant) antibody is immunoaffinity chromatography purified IgG.
Synonyms
COVID 19 Spike P681R Antibody [5H4C5] (Delta Variant) Coronavirus; Monoclonal Antibody; SARS-CoV-2 (COVID-19) Spike P681R Antibody [5H4C5] (Delta Variant); 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; anti-COVID-19 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Virus
Clonality
Monoclonal
Isotype
IgG1
Clone Number
5H4C5
Specificity
It can only detect SARS-CoV-2 Delta Variant Spike S1 protein and does not cross-react with the spike protein of other variants.
Purity/Purification
Anti-SARS-CoV-2 Spike P681R (Delta Variant) antibody is immunoaffinity chromatography purified IgG.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Applicable Applications for anti-COVID-19 antibody
ELISA (EIA)
Immunogen
Anti-SARS-CoV-2 Spike 681R (Delta Variant) antibody (MBS154843) was raised against a peptide corresponding to 12 amino acids near the cleavage site of SARS-CoV-2 Delta variant Spike protein.

The immunogen is located within 650-700 amino acids of SARS-CoV-2 Delta variant Spike protein.
Buffer
SARS-CoV-2 Spike P681R (Delta Variant) antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
SARS-CoV-2 Spike P681R (Delta Variant) antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Testing Data

(Figure 1 SARS-Cov-2 Spike P681R (Delta Variant) Antibodies Specifically Detect Delta Variant Spike S1 Protein in an ELISA Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 μg/mL, incubated at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 Spike P681R (Delta Variant) antibody, PM-9677, 1 μg/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.)

Testing Data (Figure 1 SARS-Cov-2 Spike P681R (Delta Variant) Antibodies Specifically Detect Delta Variant Spike S1 Protein in an ELISA Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 μg/mL, incubated at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 Spike P681R (Delta Variant) antibody, PM-9677, 1 μg/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.)

ELISA (EIA)

(Figure 2 ELISA Validation of Delta Variant Spike S1 Antibodies with SARS-CoV-2 Delta Variant Spike S1 Protein Coating Antigen: SARS-CoV-2 spike S1 proteins, WT, Alpha and Delta variants, 1 μg/mL, incubated at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 Spike P681R (Delta Variant) antibody, PM-9677, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.SARS-CoV-2 Spike (Delta Variant) antibody specifically detects spike S1 protein of Delta variant, but not WT and Alpha variant.)

ELISA (EIA) (Figure 2 ELISA Validation of Delta Variant Spike S1 Antibodies with SARS-CoV-2 Delta Variant Spike S1 Protein Coating Antigen: SARS-CoV-2 spike S1 proteins, WT, Alpha and Delta variants, 1 μg/mL, incubated at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 Spike P681R (Delta Variant) antibody, PM-9677, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.SARS-CoV-2 Spike (Delta Variant) antibody specifically detects spike S1 protein of Delta variant, but not WT and Alpha variant.)
Related Product Information for anti-COVID-19 antibody
SARS-CoV-2 delta variant, a variant of concern (VOC), known as B.1.617.2, was detected in India in October of 2020. However, it rapidly spread all over of the world and now it is the dominant variant in the world, which account for more than 99% of the cases. This variant carries at least 13 mutations in spike protein across the sub lineages, including L452R, D614G, P681R and K417N, which can increase the affinity to the human ACE2 receptor. Enhanced transmission of the Delta variant was observed globally, which is at least 2.5 times more contagious as the other variants. The Delta variant affects the effectiveness of COVID19 vaccine and is resistant to neutralization to some extent.
Product Categories/Family for anti-COVID-19 antibody
References
Zhang et al. JAMA 2021, 325 (13): 1324-26.

NCBI and Uniprot Product Information

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UniProt Accession #

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Product Notes

The COVID-19 (Catalog #AAA154843) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SARS-CoV-2 (COVID-19) Spike P681R Antibody [5H4C5] (Delta Variant) reacts with Virus and may cross-react with other species as described in the data sheet. AAA Biotech's COVID 19 Spike P681R Antibody [5H4C5] (Delta Variant) Coronavirus can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the COVID-19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COVID 19 Spike P681R Antibody [5H4C5] (Delta Variant) Coronavirus, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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