Figure
(>Figure 1 SARS-Cov-2 Spike 26P Antibodies Detect Spike S1 Proteins of all Variants except Gamma Variant in an ELISA Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 μg/mL, incubated at 4 ˚C overnight.
Detection Antibodies: SARS-CoV-2 Spike 26P antibody, dilution: 200 and 1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr.)
Mouse COVID-19 Spike 26P Coronavirus Monoclonal Antibody | anti-COVID-19 antibody
SARS-CoV-2 (COVID-19) Spike 26P Antibody [1C3H9]
Applications
ELISA, Western Blot
Purity
Anti-SARS-CoV-2 Spike 26P antibody is immunoaffinity chromatography purified IgG.
Synonyms
COVID-19 Spike 26P Coronavirus; Monoclonal Antibody; SARS-CoV-2 (COVID-19) Spike 26P Antibody [1C3H9]; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike 26P; anti-COVID-19 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
1C3H9
Specificity
It detects Spike S1 proteins of SARS-CoV-2 WT and variants except gamma variant.
Purity/Purification
Anti-SARS-CoV-2 Spike 26P antibody is immunoaffinity chromatography purified IgG.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Applicable Applications for anti-COVID-19 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
WB: 1 μg/mL.
Antibody validated: Western Blot in human samples. Anti-SARS-CoV-2 Spike 26P antibody detects all other SARS-CoV-2 variant Spike S1 protein, but not SARS-CoV-2 Gamma variant Spike S1 protein by ELISA. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples. Anti-SARS-CoV-2 Spike 26P antibody detects all other SARS-CoV-2 variant Spike S1 protein, but not SARS-CoV-2 Gamma variant Spike S1 protein by ELISA. All other applications and species not yet tested.
Homology
Predicted reactivity based on immunogen sequence: SARS-CoV Spike proteins: (36.4%)
Imunogen
Anti-SARS-CoV-2 Spike 26P antibody was raised against a peptide corresponding to 11 amino acids near the amino terminus of SARS-CoV-2 Spike protein.
The immunogen is located within the first 50 amino acids of SARS-CoV-2 Spike protein.
The immunogen is located within the first 50 amino acids of SARS-CoV-2 Spike protein.
Conjugation
Unconjugated
Buffer
SARS-CoV-2 Spike 26P antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
SARS-CoV-2 Spike 26P antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Figure
(>Figure 1 SARS-Cov-2 Spike 26P Antibodies Detect Spike S1 Proteins of all Variants except Gamma Variant in an ELISA Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 μg/mL, incubated at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 Spike 26P antibody, dilution: 200 and 1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr.)
Figure
(>Figure 1 SARS-Cov-2 Spike 26P Antibodies Detect Spike S1 Proteins of all Variants except Gamma Variant in an ELISA Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 μg/mL, incubated at 4 ˚C overnight.
Detection Antibodies: SARS-CoV-2 Spike 26P antibody, dilution: 200 and 1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr.)
Western Blot (WB)
Western Blot (WB)
ELISA (EIA)
(Figure 4 ELISA Validation of Spike 26P Antibodies with Mutant and WT Peptide Coating Antigen: SARS-CoV-2 spike S1 peptides: WT and Gamma variant (P.1) (9573P), 1 μg/mL, incubated at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 26P antibody, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr. SARS-CoV-2 spike 26P antibody detects WT peptide (26P, 9083P), but not Gamma variant spike S1 peptide.)
ELISA (EIA)
(Figure 4 ELISA Validation of Spike 26P Antibodies with Mutant and WT Peptide Coating Antigen: SARS-CoV-2 spike S1 peptides: WT and Gamma variant (P.1) (9573P), 1 μg/mL, incubated at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 26P antibody, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr. SARS-CoV-2 spike 26P antibody detects WT peptide (26P, 9083P), but not Gamma variant spike S1 peptide.)
Related Product Information for anti-COVID-19 antibody
In January of 2021 a new lineage of SARS-CoV-2, known as P.1 and named Gamma variant, was discovered in Japan and later spread in Brazil. It is considered a VOC (variant of concern). This variant carries 10 mutations in spike protein, including N501Y, E484K and K417T in RBD, which can increase the affinity to the human ACE2 receptor. Enhanced transmission of the Gamma variant (P.1 lineage) was observed globally, which is 3.5 times more contagious as the original one. The Gamma variant affects the effectiveness of COVID19 vaccine and is resistant to neutralization to some extent due to the immune escape E484K mutation.
Product Categories/Family for anti-COVID-19 antibody
NCBI and Uniprot Product Information
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Product Notes
The COVID-19 (Catalog #AAA154840) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's COVID-19 Spike 26P Coronavirus can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). WB: 1 μg/mL. Antibody validated: Western Blot in human samples. Anti-SARS-CoV-2 Spike 26P antibody detects all other SARS-CoV-2 variant Spike S1 protein, but not SARS-CoV-2 Gamma variant Spike S1 protein by ELISA. All other applications and species not yet tested. Researchers should empirically determine the suitability of the COVID-19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COVID-19 Spike 26P Coronavirus, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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