Sequence Information
17-Beta-Hydroxysteroid Dehydrogenase Type 3 Recombinant Protein | HSD17b3 recombinant protein
Recombinant 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3)
Reactivity
Homo sapiens (Human)
Applications
SDS-Page, Western Blot
Purity
> 90%
Synonyms
17-Beta-Hydroxysteroid Dehydrogenase Type 3; Recombinant 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3); SDR12C2; EDH17B3; Short Chain Dehydrogenase/Reductase Family 12C; Member 2; Testosterone 17-Beta-Dehydrogenase 3; Testicular 17-beta-hydroxysteroid dehydrogenase; HSD17b3 recombinant protein
Host
E.coli
Reactivity
Homo sapiens (Human)
Purity/Purification
> 90%
Form/Format
PBS, pH7.6, containing 0.01% SKL, 5% Trehalose
Concentration
Original Concentration: 120ug/mL (varies by lot)
Applicable Applications for HSD17b3 recombinant protein
Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
Application Notes
(May be suitable for use in other assays to be determined by the end user.)
Source
Prokaryotic expression
Residues
Met1~Arg310
Tags
N-terminal His Tag
Subcellular Location
Endoplasmic reticulum lumen
Traits
Freeze-dried powder
Predicted isoelectric point
8.9
Phenomenon explanation
The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Usage
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Sequence Information
Sequence Information
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Predicted Molecular Mass: 38.2kDa
Accurate Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
17-beta-hydroxysteroid dehydrogenase type 3
NCBI Official Synonym Full Names
hydroxysteroid (17-beta) dehydrogenase 3
NCBI Official Symbol
Hsd17b3
NCBI Protein Information
testosterone 17-beta-dehydrogenase 3
UniProt Protein Name
Testosterone 17-beta-dehydrogenase 3
Protein Family
UniProt Gene Name
Hsd17b3
UniProt Synonym Gene Names
Edh17b3; 17-beta-HSD 3
UniProt Entry Name
DHB3_MOUSE
Uniprot Description
HSD17B3: Favors the reduction of androstenedione to testosterone. Uses NADPH while the two other EDH17B enzymes use NADH. Defects in HSD17B3 are the cause of male pseudohermaphrodism with gynecomastia (MPH). These individuals have unambiguous female external genitalia at birth, but fail to menstruate at the time of expected puberty and instead virilize as evidenced by growth of the phallus. Breast development may or may not take place. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.
Protein type: Lipid Metabolism - androgen and estrogen; Oxidoreductase; EC 1.1.1.64
Cellular Component: intracellular membrane-bound organelle
Molecular Function: 3-alpha(17-beta)-hydroxysteroid dehydrogenase (NAD+) activity; estradiol 17-beta-dehydrogenase activity; oxidoreductase activity; testosterone 17-beta-dehydrogenase (NADP+) activity
Biological Process: lipid metabolic process; metabolic process; steroid biosynthetic process
Research Articles on HSD17b3
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Product Notes
The HSD17b3 hsd17b3 (Catalog #AAA2034334) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. The Recombinant 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's 17-Beta-Hydroxysteroid Dehydrogenase Type 3 can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Western Blot (WB). (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the HSD17b3 hsd17b3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "17-Beta-Hydroxysteroid Dehydrogenase Type 3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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