Rabbit COVID 19 Spike P26S Gamma Variant Coronavirus Polyclonal Antibody | anti-COVID-19 antibody

SARS-CoV-2 Spike P26S Antibody (Gamma Variant)

Cat. Num. AAA154818

• Reactivity: Virus; Predicted reactivity based on immunogen sequence: SARS-CoV Spike proteins: (36.4%)
• Applications: ELISA, Western Blot
• Purity: SARS-CoV-2 Spike P26S (Gamma Variant) antibody is affinity chromatography purified via peptide column.
• Synonyms: COVID 19 Spike P26S Gamma Variant Coronavirus; Polyclonal Antibody; SARS-CoV-2 Spike P26S Antibody (Gamma Variant); 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike P26S Gamma VariantSARS-CoV-2 (COVID-19) Spike Antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2); Surface Glycoprotein; Spike protein; anti-COVID-19 antibody

• Host: Rabbit
• Reactivity: Virus; Predicted reactivity based on immunogen sequence: SARS-CoV Spike proteins: (36.4%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: It can only detect SARS-CoV-2 Gamma Variant (P.1) Spike S1 protein and does not cross-react with the spike protein of other variants.
• Purity/Purification: SARS-CoV-2 Spike P26S (Gamma Variant) antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid; SARS-CoV-2 Spike P26S antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1mg/mL (varies by lot)

• Applicable Applications for anti-COVID-19 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: WB: 1ug/mL.; ; Antibody validated: Western Blot in human samples. Anti-SARS-CoV-2 Spike P26S (Gamma Variant) antibody specifically detects SARS-CoV-2 Gamma Variant (P.1) Spike S1 protein, but not SARS-CoV-2 WT and other variant Spike S1 protein by ELISA. All other applications and species not yet tested.
• Immunogen: Anti-SARS-CoV-2 Spike P26S (Gamma Variant) antibody was raised against a peptide corresponding to 11 amino acids near the amino terminus of SARS-CoV-2 Gamma variant (P.1) Spike protein.; ; The immunogen is located within the first 50 amino acids of SARS-CoV-2 Gamma variant (P.1) Spike protein.
• Conjugation: Unconjugated
• Protein GI#: 1791269090
• NCBI Organism: SARS-CoV-2
• Preparation and Storage: SARS-CoV-2 Spike P26S antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Testing Data

(Figure 1 SARS-Cov-2 Spike P26S (Gamma Variant) Antibodies Specifically Detect Gamma Variant Spike S1 Protein in ELISA Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), and delta variant (B.1.617.2), 1ug/mL, incubate at 4 degree C overnight.Detection Antibodies: SARS-CoV-2 Spike P26S (Gamma Variant) antibody, dilution: 200 and 1000 ng/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.)

ELISA (EIA)

(Figure 2 ELISA Validation of Gamma Variant Spike S1 Antibodies with SARS-CoV-2 Gamma Variant Spike S1 Protein Coating Antigen: SARS-CoV-2 spike S1 proteins WT and Gamma variant (P.1), 1ug/mL, incubate at 4 degree C overnight.Detection Antibodies: SARS-CoV-2 Spike P26S (Gamma Variant) antibody, 9573, dilution: 0.3-1000 ng/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.SARS-CoV-2 Spike P26S (Gamma Variant) antibody specifically detects Gamma variant spike S1 protein, but not WT spike S1 protein (10-300). )

Western Blot (WB)

(Figure 3 WB Validation of Gamma Variant Spike S1 Antibodies with SARS-CoV-2 Gamma Variant Spike S1 Protein Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and Gamma variant (P.1).Detection Antibodies: Spike P26S (Gamma Variant) antibody, 9573, 1ug/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.SARS-CoV-2 Spike P26S (Gamma Variant) antibody (9573) specifically detects Gamma variant spike S1 protein, but not WT spike S1 protein)

Related Product Information for anti-COVID-19 antibody

In January of 2021 a new lineage of SARS-CoV-2, known as P.1 and named as Gamma variant, was discovered in Japan and later spread in Brazil. It is considered as VOC (variant of concern). This variant carries 10 mutations in spike protein, including N501Y, E484K and K417T in RBD, which can increase the affinity to the human ACE2 receptor. Enhanced transmission of the Gamma variant (P.1 lineage) was observed globally, which is 3.5 times more contagious as the original one. The Gamma variant affects the effectiveness of COVID19 vaccine and is resistant to neutralization to some extent due to the immune escape E484K mutation.

Product Categories/Family for anti-COVID-19 antibody

Primary Antibody; Infectious Disease; COVID-19

References

Voloch et al. Journal of Virology 2021, 95 (10): e00119-21.
Wang et al. Cell Host & Microbe 2021, 29(5): 747-751.
Wang et al. Nature 592, 616-622.

NCBI and Uniprot Product Information

• NCBI GeneID: 43740568
• NCBI Official Full Name: Spike glycoprotein