ELISA (EIA)
(Figure 1 ELISA Validation of Spike 681P Antibodies with SARS-CoV-2 Spike S1 Protein
Coating Antigens: SARS-CoV-2 spike S1 proteins: WT and alpha variant (B.1.1.7, UK), 2 μg/mL, incubated at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 Spike 681P antibody, dilution: 1-3000 ng/mL, incubated at RT for 1 hr.
Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.SARS-CoV-2 spike 681P antibody detected WT spike S1 protein (10-300), but not alpha variant spike S1 protein by ELISA.)
Mouse COVID 19 Spike 681P Coronavirus Monoclonal Antibody | anti-COVID-19 antibody
SARS-CoV-2 (COVID-19) Spike 681P Antibody [8G10B1]
Reactivity
Virus
Applications
ELISA, Western Blot
Purity
SARS-CoV-2 Spike 681P antibody is immunoaffinity chromatography purified IgG.
Synonyms
COVID 19 Spike 681P Coronavirus; Monoclonal Antibody; SARS-CoV-2 (COVID-19) Spike 681P Antibody [8G10B1]; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike 681P; anti-COVID-19 antibody
Host
Mouse
Reactivity
Virus
Clonality
Monoclonal
Isotype
IgG3
Purity/Purification
SARS-CoV-2 Spike 681P antibody is immunoaffinity chromatography purified IgG.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Applicable Applications for anti-COVID-19 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
WB: 1 ug/mL. Antibody validated: Western Blot in human samples. Anti-SARS-CoV-2 Spike 681P antibody can detect SARS-CoV-2 WT Spike S1 protein, but not SARS-CoV-2 Alpha Variant (B.1.1.7, UK) S1 protein by ELISA and WB. It can also detect WT peptide, but not mutant peptide. All other applications and species not yet tested.
Homology
Predicted reactivity based on immunogen sequence: SARS-CoV Spike proteins: (25%)
Immunogen
Anti-SARS-CoV-2 (COVID-19) Spike 681P antibody was raised against a peptide corresponding to 12 amino acids near the center of SARS-CoV-2 Spike protein. The immunogen is located within 650-700 amino acids of the SARS-CoV-2 Spike protein.
Buffer
Anti-SARS-CoV-2 Spike 681P antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
SARS-CoV-2 Spike 681P antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
ELISA (EIA)
(Figure 1 ELISA Validation of Spike 681P Antibodies with SARS-CoV-2 Spike S1 Protein Coating Antigens: SARS-CoV-2 spike S1 proteins: WT and alpha variant (B.1.1.7, UK), 2 μg/mL, incubated at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 Spike 681P antibody, dilution: 1-3000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.SARS-CoV-2 spike 681P antibody detected WT spike S1 protein (10-300), but not alpha variant spike S1 protein by ELISA.)
ELISA (EIA)
(Figure 1 ELISA Validation of Spike 681P Antibodies with SARS-CoV-2 Spike S1 Protein
Coating Antigens: SARS-CoV-2 spike S1 proteins: WT and alpha variant (B.1.1.7, UK), 2 μg/mL, incubated at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 Spike 681P antibody, dilution: 1-3000 ng/mL, incubated at RT for 1 hr.
Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.SARS-CoV-2 spike 681P antibody detected WT spike S1 protein (10-300), but not alpha variant spike S1 protein by ELISA.)
Western Blot (WB)
(Figure 2 WB Validation of Spike 681P Antibodies with SARS-CoV-2 Spike S1 ProteinLoading: 30 ng of SARS-CoV-2 spike S1 proteins: WT and alpha variant (B.1.1.7, UK).Detection Antibodies: SARS-CoV-2 Spike 681P antibody, 1 μg/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.SARS-CoV-2 spike 681P antibody detected WT spike S1 protein (10-300), but not alpha variant spike S1 protein by WB.)
Western Blot (WB)
(Figure 2 WB Validation of Spike 681P Antibodies with SARS-CoV-2 Spike S1 ProteinLoading: 30 ng of SARS-CoV-2 spike S1 proteins: WT and alpha variant (B.1.1.7, UK).Detection Antibodies: SARS-CoV-2 Spike 681P antibody, 1 μg/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.SARS-CoV-2 spike 681P antibody detected WT spike S1 protein (10-300), but not alpha variant spike S1 protein by WB.)
ELISA (EIA)
(Figure 3 ELISA Validation of Spike 681P Antibodies with Mutant and WT PeptideCoating Antigen: SARS-CoV-2 spike S1 peptides: WT and alpha variant (B.1.1.7, UK) (9359P), 1 μg/mL, incubate at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 681P antibody, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.SARS-CoV-2 spike 681P antibody detected WT peptide, but not alpha variant spike S1 peptide.)
ELISA (EIA)
(Figure 3 ELISA Validation of Spike 681P Antibodies with Mutant and WT PeptideCoating Antigen: SARS-CoV-2 spike S1 peptides: WT and alpha variant (B.1.1.7, UK) (9359P), 1 μg/mL, incubate at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 681P antibody, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.SARS-CoV-2 spike 681P antibody detected WT peptide, but not alpha variant spike S1 peptide.)
Related Product Information for anti-COVID-19 antibody
In September of 2020 a new lineage of SARS-CoV-2, known as B.1.1.7 and named as Alpha variant, was discovered in the United Kingdom. This lineage developed 14 lineage-specific amino acid replacements and 3 deletions. These changes caused an increase in transmission of Alpha variant (B.1.1.7 lineage) by at least 50%, leading to increased disease severity and higher death rates. The effectiveness of COVID19 vaccines are not affected by the Alpha variant. One of the mutations associated with this lineage is a N501Y in the spike protein of the virus. It is believed that this mutation is able to increase the spike protein's affinity for the host ACE2 receptor and it has been associated with increased infectivity and virulence. B.1.1.7 viruses have also been shown to have a P681H mutation in the cleavage site of spike protein. This location is one of the residues that make up the furin proteolytic cleavage site between S1 and S2 in spike protein.
Product Categories/Family for anti-COVID-19 antibody
References
1) Duchene et al. Virus Evolution 6(2): veaa061.
2) Gu et al. Science 369(6511):1603-1607
3) Hoffmann et al. Molecular Cell 78(4):779-784.e5
4) Davies et al. Science 372(6538):eabg3055.
5) Davies et al. Nature. 593(7858):270-274.
6) Graham, et al. The Lancet Public Health. 6(5): e335-e345.
7) Horby et al. New & Emerging Threats Advisory Group. 2020;91:264-266.
2) Gu et al. Science 369(6511):1603-1607
3) Hoffmann et al. Molecular Cell 78(4):779-784.e5
4) Davies et al. Science 372(6538):eabg3055.
5) Davies et al. Nature. 593(7858):270-274.
6) Graham, et al. The Lancet Public Health. 6(5): e335-e345.
7) Horby et al. New & Emerging Threats Advisory Group. 2020;91:264-266.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The COVID-19 (Catalog #AAA154751) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SARS-CoV-2 (COVID-19) Spike 681P Antibody [8G10B1] reacts with Virus and may cross-react with other species as described in the data sheet. AAA Biotech's COVID 19 Spike 681P Coronavirus can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). WB: 1 ug/mL. Antibody validated: Western Blot in human samples. Anti-SARS-CoV-2 Spike 681P antibody can detect SARS-CoV-2 WT Spike S1 protein, but not SARS-CoV-2 Alpha Variant (B.1.1.7, UK) S1 protein by ELISA and WB. It can also detect WT peptide, but not mutant peptide. All other applications and species not yet tested. Researchers should empirically determine the suitability of the COVID-19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COVID 19 Spike 681P Coronavirus, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
