ELISA (EIA)
(Figure 1 ELISA Binding Assay of ACE2 and SARS-CoV-2 (COVID-19) Delta Variant Spike S1 Recombinant Protein Coating Antigen: SARS-CoV-2 Delta variant (B.1.671.2) spike S1 protein, 1 μg/mL, incubated at 4 ˚C overnight.Detection Antibodies: ACE2 recombinant protein, 10-120, dilution: 0.3- 1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-human HRP at 1:10,000, incubate at RT for 1 hr.)
COVID 19 Delta Variant Spike S1 (His-Avi Tag) Coronavirus Recombinant Protein | COVID-19 recombinant protein
SARS-CoV-2 (COVID-19) Delta Variant Spike S1 (His-Avi Tag) Recombinant Protein
ELISA (EIA)
(Figure 1 ELISA Binding Assay of ACE2 and SARS-CoV-2 (COVID-19) Delta Variant Spike S1 Recombinant Protein Coating Antigen: SARS-CoV-2 Delta variant (B.1.671.2) spike S1 protein, 1 μg/mL, incubated at 4 ˚C overnight.Detection Antibodies: ACE2 recombinant protein, 10-120, dilution: 0.3- 1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-human HRP at 1:10,000, incubate at RT for 1 hr.)
ELISA (EIA)
(Figure 1 ELISA Binding Assay of ACE2 and SARS-CoV-2 (COVID-19) Delta Variant Spike S1 Recombinant Protein Coating Antigen: SARS-CoV-2 Delta variant (B.1.671.2) spike S1 protein, 1 μg/mL, incubated at 4 ˚C overnight.Detection Antibodies: ACE2 recombinant protein, 10-120, dilution: 0.3- 1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-human HRP at 1:10,000, incubate at RT for 1 hr.)
SDS-Page
(Figure 2 SDS-PAGE Validation of SARS-CoV-2 (COVID-19) Delta Variant Spike S1 Recombinant Protein Loading: 1 μg SARS-CoV-2 (COVID-19) Delta variant Spike S1 recombinant protein, 95-127.Observed: 120kD)
SDS-Page
(Figure 2 SDS-PAGE Validation of SARS-CoV-2 (COVID-19) Delta Variant Spike S1 Recombinant Protein Loading: 1 μg SARS-CoV-2 (COVID-19) Delta variant Spike S1 recombinant protein, 95-127.Observed: 120kD)
ELISA (EIA)
(Figure 3 ELISA Validation of Delta Variant Spike S1 Protein with SARS-CoV-2 Delta Variant Spike Antibodies Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.671.2), 5 μg/mL, incubated at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 Delta variant spike 156-157EFdel antibody, and spike 156-157EF antibody, 9685, dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.SARS-CoV-2 Delta Variant Spike S1 protein (95-127), but not WT spike S1 protein (10-300), was specifically detected by Delta Variant spike antibody (9689), while the corresponding WT antibody detected spike S1 protein of WT, but not delta variant.)
ELISA (EIA)
(Figure 3 ELISA Validation of Delta Variant Spike S1 Protein with SARS-CoV-2 Delta Variant Spike Antibodies Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.671.2), 5 μg/mL, incubated at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 Delta variant spike 156-157EFdel antibody, and spike 156-157EF antibody, 9685, dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.SARS-CoV-2 Delta Variant Spike S1 protein (95-127), but not WT spike S1 protein (10-300), was specifically detected by Delta Variant spike antibody (9689), while the corresponding WT antibody detected spike S1 protein of WT, but not delta variant.)
ELISA (EIA)
(Figure 4 ELISA Validation of Delta Variant Spike S1 Protein with SARS-CoV-2 Delta Variant Spike Antibodies Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.671.2), 5 μg/mL, incubated at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 Delta variant spike P681R antibodies, and dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr.SARS-CoV-2 Delta Variant Spike S1 protein (95-127), but not WT spike S1 protein (10-300), was specifically detected by Delta Variant spike antibodies.)
ELISA (EIA)
(Figure 4 ELISA Validation of Delta Variant Spike S1 Protein with SARS-CoV-2 Delta Variant Spike Antibodies Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.671.2), 5 μg/mL, incubated at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 Delta variant spike P681R antibodies, and dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr.SARS-CoV-2 Delta Variant Spike S1 protein (95-127), but not WT spike S1 protein (10-300), was specifically detected by Delta Variant spike antibodies.)
Western Blot (WB)
(Figure 5 WB Validation of Delta Variant Spike S1 Protein with SARS-CoV-2 Delta Variant Spike Antibodies Loading: 20 ng of SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.671.2).Detection Antibodies: SARS-CoV-2 Delta variant spike antibody, 1 μg/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.SARS-CoV-2 Delta Variant S1 protein (95-127), but not WT spike S1 protein (10-300), was specifically detected by Delta Variant spike antibody.)
Western Blot (WB)
(Figure 5 WB Validation of Delta Variant Spike S1 Protein with SARS-CoV-2 Delta Variant Spike Antibodies Loading: 20 ng of SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.671.2).Detection Antibodies: SARS-CoV-2 Delta variant spike antibody, 1 μg/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.SARS-CoV-2 Delta Variant S1 protein (95-127), but not WT spike S1 protein (10-300), was specifically detected by Delta Variant spike antibody.)
ELISA (EIA)
(Figure 6 ELISA Validation of Delta Variant Spike S1 Protein with SARS-CoV-2 Spike S1 Antibodies Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.671.2), 5 μg/mL, incubated at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 Spike S1 antibody, dilution: 0.3-1000 ng/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.Antibody 9083 does not distinguish between WT and Delta.)
ELISA (EIA)
(Figure 6 ELISA Validation of Delta Variant Spike S1 Protein with SARS-CoV-2 Spike S1 Antibodies Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.671.2), 5 μg/mL, incubated at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 Spike S1 antibody, dilution: 0.3-1000 ng/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.Antibody 9083 does not distinguish between WT and Delta.)
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Product Notes
The COVID-19 (Catalog #AAA154847) is a Recombinant Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's COVID 19 Delta Variant Spike S1 (His-Avi Tag) Coronavirus can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), SDS-Page. Researchers should empirically determine the suitability of the COVID-19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Val 16 - Arg 685 (T19R, G142D, EF156-157d el, R158G, L452R, T478K, D614G, P681R)-His -Avi. It is sometimes possible for the material contained within the vial of "COVID 19 Delta Variant Spike S1 (His-Avi Tag) Coronavirus, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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