<
Amplication
(Synthesized standard amplifiction curve (10nM/100pM))
Human hsa-mir-25 Real-Time RT-PCR PCR Kit
hsa-mir-25 Real-Time RT-PCR Detection and U6 Calibration Kit
Reactivity
Human
Applications
Real-time RT-PCR
Synonyms
hsa-mir-25 Real-Time RT-PCR; hsa-mir-25 Real-Time RT-PCR Detection and U6 Calibration Kit; hsa-mir-25 Real-Time RT-PCR pcr kit
Reactivity
Human
Applicable Applications for hsa-mir-25 Real-Time RT-PCR pcr kit
Real-time RT-PCR
Application Notes
The Real-Time RT-PCR detection and U6 calibration Kit contains the whole reagent including the stem-loop RT primer & specific PCR primer set for microRNA quantificaion and U6 primer set as housekeeping gene for calibration. It provides a sensitive and specific method of real-time PCR for relative quantificating of miRNAs from total RNA samples. It is Ideal for validating microRNA expression profiling data and high-throughput applications. Trait lies in even highly homogized miRNAs can be accurately dicriminated ensurenced by stemloop RT primer and high specific miRNAs primer set.
Samples
miRNA, Total RNA
PCR Assay Type
2-Step qRT-PCR
Detection Range
Seven orders of magnitude
Sensitivity
10 copies of a standard mimics and as little as 25 pg of total RNA
Platform
ABI 7500 System/ViiA 7 System/StepOne/StepOnePlus/7900HT System; BioRad IQ5.0/CFX Connect; Stratagen MX3000p/4000p; Roche LightCycler 480
Components
50 rxns: It contains all reagents required to perform 50 cDNA reactions and 50 PCR reactions.
100 rxns: It contains all reagents required to perform 100 cDNA reactions and 100 PCR reactions.
200 rxns: It contains all reagents required to perform 200 cDNA reactions and 200 PCR reactions.
100 rxns: It contains all reagents required to perform 100 cDNA reactions and 100 PCR reactions.
200 rxns: It contains all reagents required to perform 200 cDNA reactions and 200 PCR reactions.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for 6 months.
Amplication
(Synthesized standard amplifiction curve (10nM/100pM))
Amplication
(Synthesized standard amplifiction curve (10nM/100pM))
Amplication #2
(Amplifiction curve in total RNA from 293T/A549 cells)
Amplication #2
(Amplifiction curve in total RNA from 293T/A549 cells)
Dissociation Curve
(Dissociation curve (Note: the minor peak on the left is the negative control))
Dissociation Curve
(Dissociation curve (Note: the minor peak on the left is the negative control))
Electrophoresis
(Electrophoresis Image (20bp marker))
Electrophoresis
(Electrophoresis Image (20bp marker))
Related Product Information for hsa-mir-25 Real-Time RT-PCR pcr kit
Detect mature miRNA hsa-mir-25 expression normalized by U6 snRNA.
Similar Products
Product Notes
The Human hsa-mir-25 Real-Time RT-PCR (Catalog #AAA825386) is a PCR Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA825386 PCR Kit recognizes Human hsa-mir-25 Real-Time RT-PCR. AAA Biotech's hsa-mir-25 Real-Time RT-PCR can be used in a range of immunoassay formats including, but not limited to, Real-time RT-PCR. The Real-Time RT-PCR detection and U6 calibration Kit contains the whole reagent including the stem-loop RT primer & specific PCR primer set for microRNA quantificaion and U6 primer set as housekeeping gene for calibration. It provides a sensitive and specific method of real-time PCR for relative quantificating of miRNAs from total RNA samples. It is Ideal for validating microRNA expression profiling data and high-throughput applications. Trait lies in even highly homogized miRNAs can be accurately dicriminated ensurenced by stemloop RT primer and high specific miRNAs primer set. Researchers should empirically determine the suitability of the hsa-mir-25 Real-Time RT-PCR for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "hsa-mir-25 Real-Time RT-PCR, PCR Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
