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ELISA (EIA) (Figure 1 ELISA Validation of Omicron Variant Spike S1 Protein with SARS-CoV-2 Omicron Variant Spike Antibodies Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and Omicron variant (B.1.1.529), 5 ug/mL, incubated at 4°C overnight. Detection Antibodies: SARS-CoV-2 Omicron Spike antibody, PM-9375, dilution: 1-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.SARS-CoV-2 Omicron Variant S1 protein (MBS154849), but not WT spike S1 protein (please inquire), was specifically detected by Omicron Variant spike antibody (MBS154756).)

COVID 19 Omicron Variant Spike S1 (His-Avi Tag) Coronavirus Recombinant Protein | COVID-19 recombinant protein

SARS-CoV-2 (COVID-19) Omicron Variant Spike S1 (His-Avi Tag) Recombinant Protein

Applications
ELISA, Western Blot, SDS-Page
Purity
> 90% as determined by Bis-Tris PAGE
Synonyms
COVID 19 Omicron Variant Spike S1 (His-Avi Tag) Coronavirus; SARS-CoV-2 (COVID-19) Omicron Variant Spike S1 (His-Avi Tag) Recombinant Protein; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; S protein RBD; Spike glycoprotein Receptor-binding domain; S glycoprotein RBD; Spike protein RBD; Delta variant spike RBD; COVID-19 recombinant protein
Ordering
For Research Use Only!
Host
HEK293 Cells
Purity/Purification
> 90% as determined by Bis-Tris PAGE
Form/Format
Liquid
Sequence
Val 16 - Arg 685 (A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H)-His-Avi
Applicable Applications for COVID-19 recombinant protein
ELISA (EIA), Western Blot (WB), SDS-Page
Fusion
His-Avi tag at C-terminus
Buffer
PBS (pH 7.4).
Preparation and Storage
Store in working aliquots at -70˚C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

ELISA (EIA)

(Figure 1 ELISA Validation of Omicron Variant Spike S1 Protein with SARS-CoV-2 Omicron Variant Spike Antibodies Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and Omicron variant (B.1.1.529), 5 ug/mL, incubated at 4°C overnight. Detection Antibodies: SARS-CoV-2 Omicron Spike antibody, PM-9375, dilution: 1-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.SARS-CoV-2 Omicron Variant S1 protein (MBS154849), but not WT spike S1 protein (please inquire), was specifically detected by Omicron Variant spike antibody (MBS154756).)

ELISA (EIA) (Figure 1 ELISA Validation of Omicron Variant Spike S1 Protein with SARS-CoV-2 Omicron Variant Spike Antibodies Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and Omicron variant (B.1.1.529), 5 ug/mL, incubated at 4°C overnight. Detection Antibodies: SARS-CoV-2 Omicron Spike antibody, PM-9375, dilution: 1-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.SARS-CoV-2 Omicron Variant S1 protein (MBS154849), but not WT spike S1 protein (please inquire), was specifically detected by Omicron Variant spike antibody (MBS154756).)

SDS-PAGE

(Figure 2 SDS-PAGE Validation of SARS-CoV-2 (COVID-19) Omicron Variant Spike S1 Recombinant Protein Loading: 2 g SARS-CoV-2 (COVID-19) Omicron variant Spike S1 recombinant protein, MBS154849. Observed: 120kD)

SDS-PAGE (Figure 2 SDS-PAGE Validation of SARS-CoV-2 (COVID-19) Omicron Variant Spike S1 Recombinant Protein Loading: 2 g SARS-CoV-2 (COVID-19) Omicron variant Spike S1 recombinant protein, MBS154849. Observed: 120kD)

Western Blot (WB)

(Figure 3 WB Validation of Omicron Variant Spike S1 Protein with SARS-CoV-2 Spike Antibodies Loading: 30 ng of SARS-CoV-2 spike S1 proteins, including WT and Omicron variant (B.1.1.529).Detection Antibodies: SARS-CoV-2 spike S1 antibody, MBS154649, 1 ug/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr. SARS-CoV-2 Omicron Variant S1 protein (MBS154849) and WT spike S1 protein (please inquire), were both detected by spike S1 antibody (MBS154649).)

Western Blot (WB) (Figure 3 WB Validation of Omicron Variant Spike S1 Protein with SARS-CoV-2 Spike Antibodies Loading: 30 ng of SARS-CoV-2 spike S1 proteins, including WT and Omicron variant (B.1.1.529).Detection Antibodies: SARS-CoV-2 spike S1 antibody, MBS154649, 1 ug/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr. SARS-CoV-2 Omicron Variant S1 protein (MBS154849) and WT spike S1 protein (please inquire), were both detected by spike S1 antibody (MBS154649).)

ELISA (EIA)

(Figure 4 ELISA Validation of Omicron Variant Spike S1 Protein with SARS-CoV-2 Spike S1 Antibodies Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and Omicron variant (B.1.1.529), 5 ug/mL, incubated at 4°C overnight. Detection Antibodies: SARS-CoV-2 Spike S1 antibody, MBS154649, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr. Antibody MBS154649 does not distinguish between WT and Omicron.)

ELISA (EIA) (Figure 4 ELISA Validation of Omicron Variant Spike S1 Protein with SARS-CoV-2 Spike S1 Antibodies Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and Omicron variant (B.1.1.529), 5 ug/mL, incubated at 4°C overnight. Detection Antibodies: SARS-CoV-2 Spike S1 antibody, MBS154649, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr. Antibody MBS154649 does not distinguish between WT and Omicron.)
Related Product Information for COVID-19 recombinant protein
SARS-CoV-2 Omicron variant, a variant of concern (VOC), known as B.1.1.529, was detected in South Africa at the end of November in 2021. However, it rapidly spread all over of the world and now it is the dominant variant in the world, which account for more than 90% of the new cases. Omicron variant spike protein carries around 30 amino acid changes, including mutations, deletions and insertions, in which the receptor binding domain (RBD) protein contains 15 mutations. Enhanced transmission of the Omicron variant was observed globally, which is at least 70 times more contagious than the other variants. The Omicron variant affects the effectiveness of COVID-19 vaccine and is resistant to neutralization (monoclonal antibody treatments) to a large extent.
Product Categories/Family for COVID-19 recombinant protein
References
Gowrisankar et al. European Physical Journal Plus, 2022, 137(1), 100.

NCBI and Uniprot Product Information

NCBI GI #
UniProt Accession #
Molecular Weight
120 kD

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Product Notes

The COVID-19 (Catalog #AAA154849) is a Recombinant Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's COVID 19 Omicron Variant Spike S1 (His-Avi Tag) Coronavirus can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), SDS-Page. Researchers should empirically determine the suitability of the COVID-19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Val 16 - Arg 685 (A67V, HV69-70del, T95I, G142D, VYY143-145 del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H)-His -Avi. It is sometimes possible for the material contained within the vial of "COVID 19 Omicron Variant Spike S1 (His-Avi Tag) Coronavirus, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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