Rabbit COVID 19 UK Variant (B.1.1.7) Spike P681H Coronavirus Polyclonal Antibody | anti-COVID-19 antibody

SARS-CoV-2 UK Variant (B.1.1.7) Spike P681H Antibody

Cat. Num. AAA154757

• Reactivity: Virus; Predicted reactivity based on immunogen sequence: SARS-CoV Spike proteins: (25%)
• Applications: ELISA, Western Blot
• Purity: SARS-CoV-2 UK Variant (B.1.1.7) Spike P681H antibody is affinity chromatography purified via peptide column.
• Synonyms: COVID 19 UK Variant (B.1.1.7) Spike P681H Coronavirus; Polyclonal Antibody; SARS-CoV-2 UK Variant (B.1.1.7) Spike P681H Antibody; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; UK Variant (B.1.1.7) Spike P681H; anti-COVID-19 antibody

• Host: Rabbit
• Reactivity: Virus; Predicted reactivity based on immunogen sequence: SARS-CoV Spike proteins: (25%)
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: SARS-CoV-2 UK Variant (B.1.1.7) Spike P681H antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid. SARS-CoV-2 UK Variant (B.1.1.7) Spike P681H antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1mg/mL (varies by lot)

• Applicable Applications for anti-COVID-19 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: WB: 1ug/mL.; Antibody validated: Western Blot in human samples. Anti-SARS-CoV-2 UK Variant (B.1.1.7) Spike P681H antibody can specifically detect SARS-CoV-2 UK Variant (B.1.1.7) Spike S1 protein, but not SARS-CoV-2 WT Spike S1 protein by ELISA. It can also detect mutant peptide (681H), but not WT peptide (681P).
• Organism: SARS-CoV-2
• Immunogen: Anti-SARS-CoV-2 UK Variant (B.1.1.7) Spike P681H antibody was raised against a peptide corresponding to 12 amino acids near the center of SARS-CoV-2 UK variant (B.1.1.7) Spike protein.; The immunogen is located within 650-700 amino acids of SARS-CoV-2 UK variant (B.1.1.7) Spike protein.
• Conjugation: Unconjugated
• Protein GI#: 1791269090
• Preparation and Storage: SARS-CoV-2 UK Variant (B.1.1.7) Spike P681H antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Testing Data

(Figure 1 ELISA Validation of Alpha Variant Spike Antibodies with Spike S1 Protein of SARS-CoV-2 Variants Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT, alpha variant (B.1.1.7, UK), beta variant (B.1.351, SA), gamma variant (P.1, Brazil), and delta variant (B.1.617.2, India), 1 μg/mL, incubate at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 Alpha Variant Spike antibody, 9359, dilution: 200-1000 ng/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.SARS-CoV-2 alpha variant spike antibody can specifically detect alpha variant spike S1 protein, but not spike S1 protein of WT and other tested variants by ELISA.)

ELISA (EIA)

(Figure 2 ELISA Validation of UK Variant Spike Antibodies with SARS-CoV-2 UK Variant Spike S1 ProteinCoating Antigen: SARS-CoV-2 spike S1 proteins, including WT and UK variant (B.1.1.7), 1 μg/mL, incubate at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 UK Variant Spike antibody, 9359, dilution: 0.1-1000 ng/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.SARS-CoV-2 UK variant spike antibody can specifically detect UK variant spike S1 protein, but not WT spike S1 protein (10-300).)

Western Blot (WB)

(Figure 3 WB Validation of UK Variant Spike Antibodies with SARS-CoV-2 UK Variant Spike S1 Protein Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and UK variant (B.1.1.7).Detection Antibodies: SARS-CoV-2 UK Variant Spike antibody, 9359, 1 μg/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.SARS-CoV-2 UK variant spike antibody can specifically detect UK variant spike S1 protein, but not WT spike S1 protein (10-300).)

ELISA (EIA)

(Figure 4 ELISA Validation of UK Variant Spike Antibodies with Mutant and WT PeptideCoating Antigen: SARS-CoV-2 spike S1 peptides, including WT and UK variant (B.1.1.7), 1 μg/mL, incubate at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 UK Variant Spike antibody, 9091, dilution: 0.1-1000 ng/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr. Immunogen region of antibody includes site 681P that was mutated in B.1.1.7. SARS-CoV-2 UK variant spike antibody (9359) can specifically detect UK variant spike S1 peptide, but not WT peptide.)

Related Product Information for anti-COVID-19 antibody

In September of 2020 a new lineage of SARS-CoV-2, known as B.1.1.7, was discovered in the United Kingdom. This lineage was found to have developed 14 lineage-specific amino acid replacements and 3 deletions prior to its discovery. The transmission of UK variant (B.1.1.7 lineage) was increased at least 50%. Increased severity and higher death rate were also found in UK variant. UK variant will not affect the effectiveness of COVID19 vaccine. One of the mutations associated with this lineage is a N501Y in the spike protein of the virus. It is believed that this mutation is able to increase the spike protein's affinity for the host ACE2 receptor and it has been associated with increased infectivity and virulence. B.1.1.7 viruses have also been shown to have a P681H in the cleavage site of spike protein. This location is one of the residues that make up the furin cleavage site between S1 and S2 in spike protein.

Product Categories/Family for anti-COVID-19 antibody

Infectious Disease; COVID-19

References

Duchene et al. Virus Evolution 6(2): veaa061.
Gu et al. Science 369(6511):1603-1607
Hoffmann et al. Molecular Cell 78(4):779-784.e5
Davies et al. Science 372(6538):eabg3055.
Davies et al. Nature. 593(7858):270-274.
Graham, et al. The Lancet Public Health. 6(5): e335-e345.
Horby et al. New & Emerging Threats Advisory Group. 2020;91:264-266.

NCBI and Uniprot Product Information

• NCBI GeneID: 43740568