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ELISA (EIA) (Figure 1 ELISA Validation of Alpha Variant Spike Antibodies with SARS-CoV-2 Alpha Variant Spike S1 ProteinCoating Antigen: SARS-CoV-2 spike S1 proteins, including WT and alpha variant (B.1.1.7, UK), 1 μg/mL, incubate at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 Alpha Variant Spike antibody, dilution: 0.3-1000 ng/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr.SARS-CoV-2 alpha variant spike antibody can specifically detect alpha variant spike S1 protein, but not WT spike S1 protein (10-300) by ELISA.)

Mouse COVID 19 P681H Mutant Specific Spike Coronavirus Monoclonal Antibody | anti-COVID-19 antibody

SARS-CoV-2 (COVID-19) P681H Mutant Specific Spike Antibody [7C11H11]

Reactivity
Virus
Applications
ELISA, Western Blot
Purity
SARS-CoV-2 (COVID-19) Alpha Variant (B.1.1.7, UK) Spike P681H Antibody is affinity chromatography purified via peptide column.
Synonyms
COVID 19 P681H Mutant Specific Spike Coronavirus; Monoclonal Antibody; SARS-CoV-2 (COVID-19) P681H Mutant Specific Spike Antibody [7C11H11]; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; P681H Mutant Specific Spike Antibody; anti-COVID-19 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Virus
Clonality
Monoclonal
Isotype
IgG2b, Kappa
Clone Number
7C11H11
Purity/Purification
SARS-CoV-2 (COVID-19) Alpha Variant (B.1.1.7, UK) Spike P681H Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Applicable Applications for anti-COVID-19 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
WB: 1 μg/mL.

Antibody validated: Western Blot in human samples. Anti-SARS-CoV-2 Alpha Variant (B.1.1.7) Spike P681H antibody can specifically detect SARS-CoV-2 Alpha Variant (B.1.1.7, UK) S1 protein, but not SARS-CoV-2 WT Spike S1 protein by ELISA and WB. It can also detect mutant peptide, but not WT peptide. All other applications and species not yet tested.
Homology
Predicted reactivity based on immunogen sequence: SARS-CoV Spike proteins: (25%)
Immunogen
Anti-SARS-CoV-2 (COVID-19) Alpha Variant (B.1.1.7, UK) Spike P681H antibody was raised against a peptide corresponding to 12 amino acids near the center of SARS-CoV-2 Alpha variant (B.1.1.7) Spike protein.

The immunogen is located within 650-700 amino acids of SARS-CoV-2 Alpha Variant (B.1.1.7, UK) Spike protein.
Buffer
Anti- Alpha Variant (B.1.1.7, UK) Spike P681H antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
SARS-CoV-2 (COVID-19) Alpha Variant (B.1.1.7, UK) Spike P681H antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ELISA (EIA)

(Figure 1 ELISA Validation of Alpha Variant Spike Antibodies with SARS-CoV-2 Alpha Variant Spike S1 ProteinCoating Antigen: SARS-CoV-2 spike S1 proteins, including WT and alpha variant (B.1.1.7, UK), 1 μg/mL, incubate at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 Alpha Variant Spike antibody, dilution: 0.3-1000 ng/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr.SARS-CoV-2 alpha variant spike antibody can specifically detect alpha variant spike S1 protein, but not WT spike S1 protein (10-300) by ELISA.)

ELISA (EIA) (Figure 1 ELISA Validation of Alpha Variant Spike Antibodies with SARS-CoV-2 Alpha Variant Spike S1 ProteinCoating Antigen: SARS-CoV-2 spike S1 proteins, including WT and alpha variant (B.1.1.7, UK), 1 μg/mL, incubate at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 Alpha Variant Spike antibody, dilution: 0.3-1000 ng/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr.SARS-CoV-2 alpha variant spike antibody can specifically detect alpha variant spike S1 protein, but not WT spike S1 protein (10-300) by ELISA.)

Western Blot (WB)

(Figure 2 WB Validation of Alpha Variant Spike Antibodies with SARS-CoV-2 Alpha Variant Spike S1 ProteinLoading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and alpha variant (B.1.1.7, UK). Detection Antibodies: SARS-CoV-2 Alpha Variant Spike antibody, 1 μg/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr.SARS-CoV-2 alpha variant spike antibody can specifically detect alpha variant spike S1 protein, but not WT spike S1 protein (10-300) by WB.)

Western Blot (WB) (Figure 2 WB Validation of Alpha Variant Spike Antibodies with SARS-CoV-2 Alpha Variant Spike S1 ProteinLoading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and alpha variant (B.1.1.7, UK). Detection Antibodies: SARS-CoV-2 Alpha Variant Spike antibody, 1 μg/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr.SARS-CoV-2 alpha variant spike antibody can specifically detect alpha variant spike S1 protein, but not WT spike S1 protein (10-300) by WB.)

ELISA (EIA)

(Figure 3 ELISA Validation of Alpha Variant Spike Antibodies with Mutant and WT PeptideCoating Antigen: SARS-CoV-2 spike S1 peptides, including WT and alpha variant (B.1.1.7, UK), 1 μg/mL, incubate at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 Alpha Variant Spike antibody, dilution: 0.1-1000 ng/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr.SARS-CoV-2 alpha variant spike antibody can specifically detect alpha variant spike S1 peptide, but not WT peptide)

ELISA (EIA) (Figure 3 ELISA Validation of Alpha Variant Spike Antibodies with Mutant and WT PeptideCoating Antigen: SARS-CoV-2 spike S1 peptides, including WT and alpha variant (B.1.1.7, UK), 1 μg/mL, incubate at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 Alpha Variant Spike antibody, dilution: 0.1-1000 ng/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr.SARS-CoV-2 alpha variant spike antibody can specifically detect alpha variant spike S1 peptide, but not WT peptide)
Related Product Information for anti-COVID-19 antibody
In September of 2020 a new lineage of SARS-CoV-2, known as B.1.1.7 and named as Alpha variant, was discovered in the United Kingdom. This lineage was found to have developed 14 lineage-specific amino acid replacements and 3 deletions prior to its discovery. The transmission of alpha variant (B.1.1.7 lineage) was increased at least 50%. Increased severity and higher death rate were also found in apha variant. Alpha variant will not affect the effectiveness of COVID19 vaccine. One of the mutations associated with this lineage is a N501Y in the spike protein of the virus. It is believed that this mutation is able to increase the spike protein's affinity for the host ACE2 receptor and it has been associated with increased infectivity and virulence. B.1.1.7 viruses have also been shown to have a P681H mutation in the cleavage site of spike protein. This location is one of the residues that make up the furin cleavage site between S1 and S2 in spike protein.
Product Categories/Family for anti-COVID-19 antibody
References
1) Duchene et al. Virus Evolution 6(2): veaa061.
2) Gu et al. Science 369(6511):1603-1607
3) Hoffmann et al. Molecular Cell 78(4):779-784.e5
4) Davies et al. Science 372(6538):eabg3055.
5) Davies et al. Nature. 593(7858):270-274.
6) Graham, et al. The Lancet Public Health. 6(5): e335-e345.
7) Horby et al. New & Emerging Threats Advisory Group. 2020;91:264-266.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #

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Product Notes

The COVID-19 (Catalog #AAA154756) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SARS-CoV-2 (COVID-19) P681H Mutant Specific Spike Antibody [7C11H11] reacts with Virus and may cross-react with other species as described in the data sheet. AAA Biotech's COVID 19 P681H Mutant Specific Spike Coronavirus can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). WB: 1 μg/mL. Antibody validated: Western Blot in human samples. Anti-SARS-CoV-2 Alpha Variant (B.1.1.7) Spike P681H antibody can specifically detect SARS-CoV-2 Alpha Variant (B.1.1.7, UK) S1 protein, but not SARS-CoV-2 WT Spike S1 protein by ELISA and WB. It can also detect mutant peptide, but not WT peptide. All other applications and species not yet tested. Researchers should empirically determine the suitability of the COVID-19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COVID 19 P681H Mutant Specific Spike Coronavirus, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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