Western Blot (WB)
(Western blot analysis of endogenous Cdc42 in human jurkat cells (lane 1) and mouse brain (lane 2), as well as detection of a 52kDa rat Cdc42 full length recombinant fusion protein (lane 3). The blots were probed with anti-Cdc42 antibody (CM4301).)
Mouse Cdc42 Monoclonal Antibody | anti-Cdc42 antibody
Cdc42 M430 Antibody
Reactivity
Human, Rat, Mouse
Applications
Western Blot, ELISA, Immunocytochemistry
Purity
Affinity Chromatography
Synonyms
Cdc42; Monoclonal Antibody; Cdc42 M430 Antibody; Cdc42 M430; anti-Cdc42 antibody
Host
Mouse
Reactivity
Human, Rat, Mouse
Clonality
Monoclonal
Isotype
IgM
Clone Number
M430
Specificity
The antibody detects a 21kDa* protein in human Jurkat cells, rat A7r5 cells, and mouse brain. The antibody also detects recombinant full length rat Cdc42 fusion protein, but does not recognize a full length human RhoA GST fusion protein.
Purity/Purification
Affinity Chromatography
Form/Format
Mouse monoclonal antibody purified with affinity chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-Cdc42 antibody
Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC)
Application Notes
ELISA: 1:2000
ICC: 1:200
WB: 1:500
ICC: 1:200
WB: 1:500
Immunogen
Clone (M430) was generated from a rat recombinant Cdc42 protein. This sequence is highly conserved in human and mouse Cdc42.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Western Blot (WB)
(Western blot analysis of endogenous Cdc42 in human jurkat cells (lane 1) and mouse brain (lane 2), as well as detection of a 52kDa rat Cdc42 full length recombinant fusion protein (lane 3). The blots were probed with anti-Cdc42 antibody (CM4301).)
Western Blot (WB)
(Western blot analysis of endogenous Cdc42 in human jurkat cells (lane 1) and mouse brain (lane 2), as well as detection of a 52kDa rat Cdc42 full length recombinant fusion protein (lane 3). The blots were probed with anti-Cdc42 antibody (CM4301).)
Immunocytochemistry (ICC)
(Immunocytochemical labeling in paraformaldehyde fixed and NP-40 permeabilized rat A7r5 cells. The cells were labeled with mouse monoclonal Anti-Cdc42 (CM4301) in the absence (Left) or presence of full length Cdc42 recombinant protein (Right), then the antibody was detected using Goat anti-Mouse secondary antibody conjugated to DyLight 594.)
Immunocytochemistry (ICC)
(Immunocytochemical labeling in paraformaldehyde fixed and NP-40 permeabilized rat A7r5 cells. The cells were labeled with mouse monoclonal Anti-Cdc42 (CM4301) in the absence (Left) or presence of full length Cdc42 recombinant protein (Right), then the antibody was detected using Goat anti-Mouse secondary antibody conjugated to DyLight 594.)
Related Product Information for anti-Cdc42 antibody
Cdc42 proteins are Rho GTPase family members, which act as molecular switches in regulating a variety of biological response pathways including cell motility, cell-cycle progression, gene transcription, and cell transformation. Cdc42 GTPases regulate molecular events by cycling from the inactive GDP-bound state to active GTP-bound forms. The ratio of GDP versus GTP, which is bound to the GTPase, is determined by the opposing effects of guanine nucleotide exchange factors and GTPase activating proteins. Active (GTP-bound) Cdc42 binds to the p21-binding domain of p21-activated protein kinase 1 (PAK1) to regulate downstream signaling cascades.
References
Erickson, J.W. & Cerione, R.A. (2001) Curr. Opin. Cell. Biol. 13(2):153.
Munemitsu, S. et al. (1990) Mol. Cell. Biol. 10:5977.
Shinjo, K. et al. (1990) PNAS (USA) 87:9853.
Munemitsu, S. et al. (1990) Mol. Cell. Biol. 10:5977.
Shinjo, K. et al. (1990) PNAS (USA) 87:9853.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,259 Da
NCBI Official Full Name
cell division control protein 42 homolog
NCBI Official Synonym Full Names
cell division cycle 42
NCBI Official Symbol
Cdc42
NCBI Protein Information
cell division control protein 42 homolog; cell division cycle 42 homolog; cell division cycle 42 (GTP binding protein)
UniProt Protein Name
Cell division control protein 42 homolog
Protein Family
UniProt Gene Name
Cdc42
UniProt Entry Name
CDC42_RAT
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Product Notes
The Cdc42 cdc42 (Catalog #AAA474262) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Cdc42 M430 Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Cdc42 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC). ELISA: 1:2000 ICC: 1:200 WB: 1:500. Researchers should empirically determine the suitability of the Cdc42 cdc42 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cdc42, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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