Testing Data
Cdc42 G15A Agarose Beads (Active Cdc42-GEF) Accessory Reagent
Cdc42 G15A Agarose Beads (Active Cdc42-GEF)
Synonyms
Cdc42 G15A Agarose Beads (Active Cdc42-GEF); Cdc42 G15A Agarose Beads (Active Cdc42-GEF) accessory reagent
Quantity
800 uL of 50% Agarose slurry, 1 mg/mL Cdc42 G15A in 1X PBS, 50% Glycerol
Preparation and Storage
-20 degree C. Shelf Life: 1 year from receipt under proper storage conditions; avoid multiple freeze thaw cycles
Testing Data
Testing Data
Related Product Information for Cdc42 G15A Agarose Beads (Active Cdc42-GEF) accessory reagent
• Pull down the active form of Cdc42-GEF
• Colored for easy visualization
Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways. Cdc42, a 21 kDa protein, belongs to the family of Rho GTPases regulating a variety of biological response pathways that include cell motility, cell division, gene transcription, and cell transformation. Like other small GTPases, Cdc42 influences molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. Cycling between the GDP-bound and GTP-bound state is regulated primarily by two distinct families of proteins: guanine nucleotide exchange factors (GEFs) activate Rho proteins by catalyzing the exchange of GDP for GTP, the GTPase activating proteins or GAPs negatively regulate GTPase function by stimulating GTP hydrolysis.
Similar to Ras mutants, constitutively active or dominant negative Rho GTPase mutants have been used to bind to Rho-GAP and effectors or to Rho-GEFs, respectively. A nucleotide-free GTPase has also been shown to form a high affinity binary complex with Rho-GEFs. Cdc42 G15A Agarose beads to selectively isolate and pull-down the active form of Cdc42-GEFs from purified samples or endogenous lysates. Subsequently, the precipitated Cdc42-GEF is detected by western blot analysis using an anti-Cdc42-GEF antibody.
• Colored for easy visualization
Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways. Cdc42, a 21 kDa protein, belongs to the family of Rho GTPases regulating a variety of biological response pathways that include cell motility, cell division, gene transcription, and cell transformation. Like other small GTPases, Cdc42 influences molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. Cycling between the GDP-bound and GTP-bound state is regulated primarily by two distinct families of proteins: guanine nucleotide exchange factors (GEFs) activate Rho proteins by catalyzing the exchange of GDP for GTP, the GTPase activating proteins or GAPs negatively regulate GTPase function by stimulating GTP hydrolysis.
Similar to Ras mutants, constitutively active or dominant negative Rho GTPase mutants have been used to bind to Rho-GAP and effectors or to Rho-GEFs, respectively. A nucleotide-free GTPase has also been shown to form a high affinity binary complex with Rho-GEFs. Cdc42 G15A Agarose beads to selectively isolate and pull-down the active form of Cdc42-GEFs from purified samples or endogenous lysates. Subsequently, the precipitated Cdc42-GEF is detected by western blot analysis using an anti-Cdc42-GEF antibody.
Product Categories/Family for Cdc42 G15A Agarose Beads (Active Cdc42-GEF) accessory reagent
References
1. Arthur, W.T., Ellerbroek, S.M., Der, C.J., and Burridge K. (2002) J. Biol. Chem. 277, 42964-42972
2. Garcia-Mata R., Wennerberg, K., Arthur, W.T., Noren, N.K., Ellenbroek, S.M., and Burridge K. (2006) Methods Enzymol. 406, 425-437.
2. Garcia-Mata R., Wennerberg, K., Arthur, W.T., Noren, N.K., Ellenbroek, S.M., and Burridge K. (2006) Methods Enzymol. 406, 425-437.
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Product Notes
The Cdc42 G15A Agarose Beads (Active Cdc42-GEF) (Catalog #AAA168388) is an Accessory Reagent and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Cdc42 G15A Agarose Beads (Active Cdc42-GEF), Accessory Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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