Rabbit Arp3 Polyclonal Antibody | anti-Arp3 antibody

Arp3 (C-Terminal Region)

Cat. Num. AAA474085

• Gene Names: ACTR3; ARP3
• Reactivity: Human, Rat, Mouse, Chicken, Frog
• Applications: Western Blot, ELISA, Immunocytochemistry
• Purity: Affinity Purified
• Synonyms: Arp3; Polyclonal Antibody; Arp3 (C-Terminal Region); ACTR3; anti-Arp3 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse, Chicken, Frog
• Clonality: Polyclonal
• Specificity: This antibody was affinity purified using Arp3 (C-terminal region) peptide (without carrier). The antibody detects a 47 kDa* doublet corresponding to the molecular mass of Arp3 on SDS-PAGE immunoblots of human A431, Jurkat, and HeLa cells, and in rat PC12 cells.
• Purity/Purification: Affinity Purified
• Form/Format: Supplied in 100ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
• Sequence Length: 418

• Applicable Applications for anti-Arp3 antibody: Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC)
• Application Notes: ELISA: 1:2000; ICC: 1:250; WB: 1:1000
• Immunogen: Arp3 (C-terminal region) synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human Arp3. This peptide sequence is highly conserved in rat, mouse, chicken, fish, and yeast Arp3 proteins.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot of human A431 (lane 1), Jurkat (lane 2), and HeLa (lane 3) cells. The blots were probed with rabbit polyclonal anti-Arp3 (C-terminal region) antibody at 1:1000 (lanes 1-3).)

Immunocytochemistry (ICC)

(Immunocytochemical labeling of Arp3 in aldehyde-fixed and NP-40-permeabilized rat PC12 cells differentiated with NGF. The cells were labeled with rabbit polyclonal anti-Arp3 (C-terminal region) (AP4581) in the absence (left) or presence (right) of blocking peptide (AX4585). The antibody was detected using appropriate secondary antibody conjugated to DyLight 594.)

Related Product Information for anti-Arp3 antibody

Cellular morphology, adhesion, and motility occur through dynamic reorganization of actin-based superstructures. Actin-binding proteins are critical for regulating actin polymerization and superstructure formation. The Arp2/3 complex is an actin polymerization-inducing complex that includes Arp2, Arp3, p41-Arc, p34-Arc, p21-Arc, p20-Arc, and p16-Arc. Several nucleation promoting factors, such as WASP and coronin, regulate the activity of the Arp2/3 complex. In addition, the Arp2/3 complex may be regulated by phosphorylation of specific subunits in the complex. Arp2 has two phosphosites, Thr-237 and Thr-238, that are evolutionarily conserved, and are phosphorylated along with Tyr-202 in response to growth factor stimulation. These phosphorylation events may regulate binding to the pointed end of actin filaments, and alanine substitutions of these Arp2 phosphosites inhibit membrane protrusions. Thus, phosphorylation may be another mode of Arp2/3 complex regulation in addition to the activity of nucleation-promoting factors.

References

Kelleher, J.F. et al. (1995). J Cell Biol. 131(2):385.LeClaire, L.L. et al (2008). J Cell Biol. 182(4):647.Soderling, S.H. (2009). Sci Signal. 2(55):pe5 (Review).

NCBI and Uniprot Product Information

• NCBI GI #: 5031573
• NCBI GeneID: 10096
• NCBI Accession #: NP_005712.1
• NCBI GenBank Nucleotide #: NM_005721.4
• UniProt Accession #: P61158
• Molecular Weight: 47 kDa
• NCBI Official Full Name: actin-related protein 3 isoform 1
• NCBI Official Synonym Full Names: actin related protein 3
• NCBI Official Symbol: ACTR3
• NCBI Official Synonym Symbols: ARP3
• NCBI Protein Information: actin-related protein 3
• UniProt Protein Name: Actin-related protein 3
• Protein Family: Actin-related protein
• UniProt Gene Name: ACTR3
• UniProt Synonym Gene Names: ARP3
• UniProt Entry Name: ARP3_HUMAN

NCBI Description

The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Mar 2013]

Uniprot Description

Arp3: Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. Plays a role in ciliogenesis. Component of the Arp2/3 complex composed of ARP2, ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC. Interacts with WHDC1. Belongs to the actin family. ARP3 subfamily.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q14.1

Cellular Component: Golgi membrane; Arp2/3 protein complex; focal adhesion; hemidesmosome; membrane; lamellipodium; excitatory synapse; actin filament; podosome; intercellular junction; cytosol; actin cytoskeleton

Molecular Function: actin filament binding; protein binding; structural constituent of cytoskeleton; ATP binding

Biological Process: positive regulation of filopodium formation; meiotic chromosome movement towards spindle pole; axon guidance; regulation of myosin II filament assembly or disassembly; establishment and/or maintenance of cell polarity; positive regulation of dendrite morphogenesis; cytokinesis after meiosis; response to carbohydrate stimulus; spindle localization; response to antibiotic; asymmetric cell division; ephrin receptor signaling pathway; innate immune response; cell motility