Western Blot (WB)
((0.3ug/ml) staining of lysates of cell line HepG2 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat FOXA1/HNF3A Polyclonal Antibody | anti-FOXA1 antibody
Goat anti-FOXA1/HNF3A Antibody
Immunohistochemistry: In paraffin embedded Human Prostate shows preferential nuclear staining in the epithelial cells. Recommended concentration, 2-4ug/ml.
Western Blot: Approx 50kDa band observed in lysates of cell line HepG2 (calculated MW of 49.1kDa according to NP_004487.2). Recommended concentration: 0.3-1ug/ml. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunizing peptide.
Chromatin Immunoprecipitation: This antibody has been successfully used for mouse embryonic stem cells and it has been confirmed in ChIP by anonymous customers.
Western Blot (WB)
((0.3ug/ml) staining of lysates of cell line HepG2 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of lysates of cell line HepG2 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. [provided by RefSeq, Jul 2008]
Uniprot Description
FOXA1: Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3'. Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles. Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of p27Kip1, alone or in conjunction with BRCA1. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis- acting regulatory regions of these genes. Involved in glucose homeostasis. Binds DNA as a monomer. Interacts with FOXA2. Interacts with NKX2-1. Interacts with HDAC7. Interacts with the histone H3-H4 heterodimer. Associates with nucleosomes containing histone H2A. Interacts with AR. Interacts with NR0B2. Highly expressed in prostate and ESR1-positive breast tumors. Overexpressed in esophageal and lung adenocarcinomas.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 14q21.1
Cellular Component: microvillus; nucleolus; nucleus
Molecular Function: DNA binding; protein domain specific binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding
Biological Process: anatomical structure formation; chromatin remodeling; dorsoventral neural tube patterning; glucose homeostasis; hormone metabolic process; negative regulation of transcription from RNA polymerase II promoter; neuron fate specification; Notch signaling pathway; positive regulation of estrogen receptor signaling pathway; positive regulation of mitotic cell cycle; positive regulation of neuron differentiation; positive regulation of smoothened signaling pathway; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; response to estradiol stimulus; transcription from RNA polymerase II promoter
Research Articles on FOXA1
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Product Notes
The FOXA1 foxa1 (Catalog #AAA420148) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-FOXA1/HNF3A Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's FOXA1/HNF3A can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP). Peptide ELISA: Antibody detection limit dilution 1: 64000. Immunohistochemistry: In paraffin embedded Human Prostate shows preferential nuclear staining in the epithelial cells. Recommended concentration, 2-4ug/ml. Western Blot: Approx 50kDa band observed in lysates of cell line HepG2 (calculated MW of 49.1kDa according to NP_004487.2). Recommended concentration: 0.3-1ug/ml. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunizing peptide. Chromatin Immunoprecipitation: This antibody has been successfully used for mouse embryonic stem cells and it has been confirmed in ChIP by anonymous customers. Researchers should empirically determine the suitability of the FOXA1 foxa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: GVYSRPVLNT S. It is sometimes possible for the material contained within the vial of "FOXA1/HNF3A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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