Western Blot (WB)
(Western blot of adult mouse brain. The blots were probed with anti-nSMase2 (C-terminal region) rabbit polyclonal antibody at 1:2000 (lane 1), 1:1000 (lane 2), or 1:500 in the absence (lane 3) or presence of nSMase2 blocking peptide (SX4065) (lane 4).)
Rabbit nSMase2 Polyclonal Antibody | anti-nSMase2 antibody
nSMase2 (C-terminal region) Antibody
ICC: 1:100
WB: 1:1000
Western Blot (WB)
(Western blot of adult mouse brain. The blots were probed with anti-nSMase2 (C-terminal region) rabbit polyclonal antibody at 1:2000 (lane 1), 1:1000 (lane 2), or 1:500 in the absence (lane 3) or presence of nSMase2 blocking peptide (SX4065) (lane 4).)
Western Blot (WB)
(Western blot of adult mouse brain. The blots were probed with anti-nSMase2 (C-terminal region) rabbit polyclonal antibody at 1:2000 (lane 1), 1:1000 (lane 2), or 1:500 in the absence (lane 3) or presence of nSMase2 blocking peptide (SX4065) (lane 4).)
Immunocytochemistry (ICC)
(Immunocytochemical labeling of nSMase2 in aldehyde-fixed and NP-40-permeabilized differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-nSMase2 (SP4061) antibody in the absence (Left) or presence (Right) of blocking peptide (SX4065). The antibody was detected using appropriate secondary antibody conjugated to DyLight 594. The cells were counterstained with Sytox green to label nuclei.)
Immunocytochemistry (ICC)
(Immunocytochemical labeling of nSMase2 in aldehyde-fixed and NP-40-permeabilized differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-nSMase2 (SP4061) antibody in the absence (Left) or presence (Right) of blocking peptide (SX4065). The antibody was detected using appropriate secondary antibody conjugated to DyLight 594. The cells were counterstained with Sytox green to label nuclei.)
Stoffel, W. et al. (2005) Proc Natl Acad Sci U S A. 102(12):4554.
Hofmann, K. et al. (2000) Proc Natl Acad Sci U S A. 97(11):5895.
NCBI and Uniprot Product Information
Uniprot Description
Function: Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Regulates the cell cycle by acting as a growth suppressor in confluent cells. Probably acts as a regulator of postnatal development and participates in bone and dentin mineralization. Ref.1 Ref.3 Ref.4
Catalytic activity: Sphingomyelin + H2O = N-acylsphingosine + phosphocholine.
Cofactor: Magnesium. Ref.1 Ref.4
Enzyme regulation: Activated by unsaturated fatty acids and phosphatidylserine. Ref.1
Pathway: Lipid metabolism; sphingolipid metabolism.
Subcellular location: Golgi apparatus membrane; Lipid-anchor. Cell membrane; Lipid-anchor. Note: May localize to detergent-resistant subdomains of Golgi membranes of hypothalamic neurosecretory neurons. According to Ref.4, it localizes to plasma membrane in confluent contact-inhibited cells. Ref.1 Ref.4
Tissue specificity: Predominantly expressed in brain. Ref.1
Developmental stage: Up-regulated during G0/G1 phases.
Post-translational modification: Palmitoylated, palmitoylation-deficient proteins are targeted for lysosomal degradation
By similarity.
Sequence similarities: Belongs to the neutral sphingomyelinase family.
Biophysicochemical propertiespH dependence:Optimum pH is 7.5.
Research Articles on nSMase2
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Product Notes
The nSMase2 smpd3 (Catalog #AAA474603) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The nSMase2 (C-terminal region) Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's nSMase2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC). ELISA: 1:2000 ICC: 1:100 WB: 1:1000. Researchers should empirically determine the suitability of the nSMase2 smpd3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "nSMase2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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