Human Platelet Derived Growth Factor Subunit A (PDGFA) ELISA Kit | PDGFA elisa kit

Human Platelet Derived Growth Factor Subunit A (PDGFA) ELISA Kit

Cat. Num. AAA2701914

• Gene Names: PDGFA; PDGF1; PDGF-A
• Reactivity: Human
• Synonyms: Platelet Derived Growth Factor Subunit A (PDGFA); Human Platelet Derived Growth Factor Subunit A (PDGFA) ELISA Kit; PDGF-A; PDGF1; Platelet Derived Growth Factor Alpha Polypeptide; PDGFA elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Platelet Derived Growth Factor Subunit A (PDGFA).; No significant cross-reactivity or interference between Platelet Derived Growth Factor Subunit A (PDGFA) and analogues was observed.
• Sequence Length: 196

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.056ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Platelet Derived Growth Factor Subunit A (PDGFA) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Platelet Derived Growth Factor Subunit A (PDGFA) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PDGFA elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Platelet Derived Growth Factor Subunit A (PDGFA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Platelet Derived Growth Factor Subunit A (PDGFA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Platelet Derived Growth Factor Subunit A (PDGFA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Platelet Derived Growth Factor Subunit A (PDGFA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 15208656
• NCBI GeneID: 5154
• NCBI Accession #: NP_148983.1
• NCBI GenBank Nucleotide #: NM_033023.4
• UniProt Accession #: P04085; B5BU73
• Molecular Weight: 22,253 Da
• NCBI Official Full Name: platelet-derived growth factor subunit A isoform 2 preproprotein
• NCBI Official Synonym Full Names: platelet derived growth factor subunit A
• NCBI Official Symbol: PDGFA
• NCBI Official Synonym Symbols: PDGF1; PDGF-A
• NCBI Protein Information: platelet-derived growth factor subunit A
• UniProt Protein Name: Platelet-derived growth factor subunit A
• Protein Family: Platelet-derived growth factor
• UniProt Gene Name: PDGFA
• UniProt Synonym Gene Names: PDGF1; PDGF subunit A

NCBI Description

This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit A, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit B. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

Uniprot Description

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB ().