Mouse Cdc42 Monoclonal Antibody | anti-CDC42 antibody

Cdc42

Cat. Num. AAA470007

• Gene Names: CDC42; G25K; CDC42Hs
• Reactivity: Human, Rat, Mouse
• Applications: ELISA, Western Blot, Immunocytochemistry
• Synonyms: Cdc42; Monoclonal Antibody; Cell Division Cycle 42; GTP-Binding Protein 25-KD; G25K; anti-CDC42 antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: M152
• Specificity: The antibody detects a 21 kDa protein in human Jurkat cells and mouse brain. It does not recognize a human RhoA GST fusion protein.
• Form/Format: Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
• Sequence Length: 136

• Applicable Applications for anti-CDC42 antibody: ELISA, Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: ELISA: 1:2000; Western Blot: 1:500; Immunocytochemistry: 1:50; ; End user should determine optimal dilution for their particular applications and experiments. Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
• Immunogen: Clone (M152) was generated from a rat recombinant Cdc42 protein. This sequence is highly conserved in human and mouse Cdc42.
• Preparation and Storage: Store at –20°C. Stable for 1 year.

Immunocytochemistry (ICC)

(Immunocytochemical labeling in rat PC12 cells grown for 4 days on poly-D-lysine-coated plates in the presence (200 ng/ml) or absence (Control) of Nerve Growth Factor (NGF). Anti-Cdc42 (CM1521) was used at 1:50 dilution followed by labeling with donkey anti-mouse:Cy2. )

Western Blot (WB)

(Western blot analysis of human jurkat cells (lanes 1 & 2) and mouse brain (lanes 3 & 4). The blots were probed with anti-Cdc42 antibody at 1:125 (lanes 1 & 3) or 1:500 (lanes 2 & 4).)

Related Product Information for anti-CDC42 antibody

Cdc42 proteins are Rho GTPase family members, which act as molecular switches in regulating a variety of biological response pathways including cell motility, cell-cycle progression, gene transcription, and cell transformation. Cdc42 GTPases regulate molecular events by cycling from the inactive GDP-bound state to active GTP-bound forms. The ratio of GDP versus GTP, which is bound to the GTPase, is determined by the opposing effects of guanine nucleotide exchange factors and GTPase activating proteins. Active (GTP-bound) Cdc42 binds to the p21-binding domain of p21-activated protein kinase 1 (PAK1) to regulate downstream signaling cascades.

References

• Munemitsu, S. et al. (1990) Mol. Cell. Biol. 10:5977.
• Shinjo, K. et al. (1990) PNAS (USA) 87:9853.
• Erickson, J.W. & Cerione, R.A. (2001) Curr. Opin. Cell. Biol. 13(2):153.

NCBI and Uniprot Product Information

• NCBI GI #: 56202836
• NCBI GeneID: 998
• NCBI Accession #: CAI19851.1
• NCBI GenBank Nucleotide #: AL031281.6
• UniProt Accession #: Q5JYX0; P60953; Q9UJM0; Q9UJM1
• NCBI Official Full Name: cell division cycle 42 (GTP binding protein, 25kDa)
• NCBI Official Synonym Full Names: cell division cycle 42 (GTP binding protein, 25kDa)
• NCBI Official Symbol: CDC42
• NCBI Official Synonym Symbols: G25K; CDC42Hs
• NCBI Protein Information: cell division control protein 42 homolog; OTTHUMP00000002834; OTTHUMP00000002926; G25K GTP-binding protein; GTP-binding protein, 25kD; growth-regulating protein; small GTP binding protein CDC42; dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)); dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD))
• UniProt Protein Name: Cell division cycle 42 (GTP binding protein, 25kDa)
• Protein Family: Cdc42
• UniProt Gene Name: CDC42
• UniProt Entry Name: Q5JYX0_HUMAN

NCBI Description

The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq]

Uniprot Description

Sequence similarities: Belongs to the small GTPase superfamily. Rho family. RuleBase RU003317