Western Blot (WB)
(Western blot analysis of endogenous Cdc42 in human jurkat cells (lane 1) and mouse brain (lane 2), as well as detection of a 52kDa rat Cdc42 full length recombinant fusion protein (lane 3). The blots were probed with anti-Cdc42 antibody (CM4301).)
Cdc42 recombinant protein
Cdc42 Recombinant Fusion Protein
Applications
Western Blot
Synonyms
Cdc42; Cdc42 Recombinant Fusion Protein; Cdc42 recombinant protein
Host
Lysate
Form/Format
Cdc42 recombinant protein lysate is supplied at a concentration of 20ng/ul in electrophoresis sample buffer (62.5mM Tris pH6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% beta-mercaptoethanol).
Applicable Applications for Cdc42 recombinant protein
Western Blot (WB)
Application Notes
WB: 20ul/lane
Preparation and Storage
Store at -20 degree C. Do not boil or dilute. Stable for 1 year.
Western Blot (WB)
(Western blot analysis of endogenous Cdc42 in human jurkat cells (lane 1) and mouse brain (lane 2), as well as detection of a 52kDa rat Cdc42 full length recombinant fusion protein (lane 3). The blots were probed with anti-Cdc42 antibody (CM4301).)
Western Blot (WB)
(Western blot analysis of endogenous Cdc42 in human jurkat cells (lane 1) and mouse brain (lane 2), as well as detection of a 52kDa rat Cdc42 full length recombinant fusion protein (lane 3). The blots were probed with anti-Cdc42 antibody (CM4301).)
Related Product Information for Cdc42 recombinant protein
Cdc42 proteins are Rho GTPase family members, which act as molecular switches in regulating a variety of biological response pathways including cell motility, cell-cycle progression, gene transcription, and cell transformation. Cdc42 GTPases regulate molecular events by cycling from the inactive GDP-bound state to active GTP-bound forms. The ratio of GDP versus GTP, which is bound to the GTPase, is determined by the opposing effects of guanine nucleotide exchange factors and GTPase activating proteins. Active (GTP-bound) Cdc42 binds to the p21-binding domain of p21-activated protein kinase 1 (PAK1) to regulate downstream signaling cascades.Rat Cdc42 full length protein (21kDa) was generated as a fusion protein (52kDa) from a pGEM expression vector system. The Cdc42 fusion protein is detected by mouse monoclonal anti-Cdc42 and anti-Cdc42 .
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Product Notes
The Cdc42 (Catalog #AAA474220) is a Recombinant Protein produced from Lysate and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Cdc42 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 20ul/lane. Researchers should empirically determine the suitability of the Cdc42 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cdc42, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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