CD96 recombinant protein

Recombinant Mouse CD96 Protein (His tag)

Cat. Num. AAA2546801

• Gene Names: Cd96; Tactile; 1700109I12Rik
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: CD96; Recombinant Mouse CD96 Protein (His tag); 1700109I12Rik; Tactile; CD96 recombinant protein

• Host: Human Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 602

• Application Notes: The secreted recombinant mouse CD96 comprises 526 amino acids and has a calculated molecular mass of 58 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 130-140 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Val 22
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CD96 recombinant protein

Background: The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. The CD155 ligand CD96 is a member of the Ig superfamily. It's a immunoglobulin-like protein tentatively allocated to the repertoire of human NK receptors. NK cells recognize poliovirus receptor (PVR), a nectins and nectin-like protein family member serve to mediate cell-cell adhesion, cell migration, with the presence of an additional receptor, CD96. CD96 promotes NK cell adhesion to target cells expressing PVR, stimulates cytotoxicity of activated NK cells, and mediates acquisition of PVR from target cells. The effect the cells with mutated CD96 protein lost adhesion and growth activities indicates that CD96 mutations may cause a form of the C syndrome by interfering with cell adhesion and growth.

Description: A DNA sequence encoding the mouse CD96 (Q3U0X8) extracellular domain (Met 1-Met 536) was expressed, fused with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 160333634
• NCBI GeneID: 84544
• NCBI Accession #: NP_115854.2
• NCBI GenBank Nucleotide #: NM_032465.2
• UniProt Accession #: Q3U0X8; Q7TMW0; Q8C9U6; Q99M67
• Molecular Weight: 67,133 Da
• NCBI Official Full Name: T-cell surface protein tactile
• NCBI Official Synonym Full Names: CD96 antigen
• NCBI Official Symbol: Cd96
• NCBI Official Synonym Symbols: Tactile; 1700109I12Rik
• NCBI Protein Information: T-cell surface protein tactile
• UniProt Protein Name: T-cell surface protein tactile
• Protein Family: T-cell surface protein tactile
• UniProt Gene Name: Cd96

Uniprot Description

CD96: May be involved in adhesive interactions of activated T and NK cells during the late phase of the immune response. Promotes NK cell-target adhesion by interacting with PVR present on target cells. May function at a time after T and NK cells have penetrated the endothelium using integrins and selectins, when they are actively engaging diseased cells and moving within areas of inflammation. Defects in CD96 are a cause of C syndrome (CSYN); also called Opitz trigonocephaly syndrome. This syndrome is characterized by trigonocephaly and associated anomalies, such as unusual facies, wide alveolar ridges, multiple buccal frenula, limb defects, visceral anomalies, redundant skin, psychomotor retardation and hypotonia. A chromosomal aberration involving CD96 has been found in a patient with C syndrome. Translocation t(3;18)(q13.13;q12.1). CD96 gene was located at the 3q13.13 breakpoint. Precise structural analysis around the breakpoint showed that the gene was disrupted by the translocation in exon 5, probably leading to premature termination or loss of expression of CD96 protein. No gene was detected at the chromosome 18 breakpoint. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16|16 B5

Cellular Component: adherens junction; cytoplasm

Molecular Function: protein binding

Biological Process: cell-matrix adhesion; cytokine production during acute inflammatory response; negative regulation of interferon-gamma production; negative regulation of natural killer cell cytokine production; response to lipopolysaccharide