TIGIT recombinant antibody

Anti-TIGIT [1B4]

Cat. Num. AAA488487

• Gene Names: Tigit; Vstm3
• Reactivity: Mouse
• Applications: ELISA, Flow Cytometry, Functional Assay
• Purity: Purified
• Synonyms: TIGIT; Monoclonal Recombinant Antibody; Anti-TIGIT [1B4]; VSTM3; T cell Ig and ITIM domain; T cell immunoreceptor with Ig and ITIM domains; V-set and transmembrane domain-containing protein 3; WUCAM; TIGIT recombinant antibody

• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1 lambda
• Clone Number: 1B4
• Specificity: This antibody is specific for murine TIGIT.
• Purity/Purification: Purified
• Form/Format: PBS with 0.02% Proclin 300.
• Sequence Length: 249

• Applicable Applications for TIGIT recombinant antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Blocking (BL)
• Antibody Type: Recombinant Antibody
• Antibody Species: Mouse
• Original Species Name: Mouse
• Immunogen: This antibody was raised by immunising TIGIT-/- mice with recombinant murine TIGIT tetramers.
• Clone Note: The binding specificity of this antibody for TIGIT has been confirmed in ELISA analysis and by flow cytometry using P815 cells transfected with mouse TIGIT and activated mouse splenocytes (Dixon et al, 2018). This antibody also specifically stains activated primary wild-type T cells (Dixon et al, 2018). This antibody fully blocks CD155 binding to TIGIT, and acts as a TIGIT blocking antibody in vivo. Administration of this antibody increases experimental autoimmune encephalomyelitis (EAE) severity, and retards the growth of established MC38 colon carcinoma and GL261 glioblastoma (Dixon et al, 2018). This antibody synergises with anti-PD-1 blocking antibodies to achieve complete tumour regression in colon carcinoma models (Dixon et al, 2018). The original mouse IgG1 antibody does not deplete TIGIT-expressing CD4+ or CD8+ T cells in vivo, nor affect T cell proliferation in vitro (Dixon et al, 2018).
• Buffer Composition: PBS only with 0.02% Proclin 300.
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

Western Blot (WB)

(Western Blot using anti-TIGIT antibody 1B4 RAW-264.7 cells (A), mouse spleen (B) and mouse thymus (C) lysates (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of 1B4 at 0.3 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)

Related Product Information for TIGIT recombinant antibody

Recombinant monoclonal antibody to TIGIT. Manufactured using the recombinant platform with variable regions (i.e. specificity) from the hybridoma 1B4.

Product Categories/Family for TIGIT recombinant antibody

Autoimmunity; Oncology; Synergy

References

Dixon et al. Functional Anti-TIGIT Antibodies Regulate Development of Autoimmunity and Antitumor Immunity J Immunol. 2018 Apr 15;200(8):3000-3007 PMID:29500245
Original Published Journal: J Immunol. 2018 Apr 15;200(8):3000-3007
Publication Note: Describes the original generation of this antibody, and the establishment of its TIGIT-blocking function.

NCBI and Uniprot Product Information

• NCBI GI #: 224493410
• NCBI GeneID: 100043314
• UniProt Accession #: P86176
• NCBI Official Full Name: T-cell immunoreceptor with Ig and ITIM domains
• NCBI Official Synonym Full Names: T cell immunoreceptor with Ig and ITIM domains
• NCBI Official Symbol: Tigit
• NCBI Official Synonym Symbols: Vstm3
• NCBI Protein Information: T-cell immunoreceptor with Ig and ITIM domains
• UniProt Protein Name: T-cell immunoreceptor with Ig and ITIM domains
• Protein Family: T-cell immunoreceptor with Ig and ITIM domains
• UniProt Gene Name: Tigit

Uniprot Description

Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.