Western Blot (WB)
(Western Blot using anti-CD155 antibody 3F1 (MBS488666). Mouse thymus lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric rabbit IgG version of 3F1 (MBS488666) at 0.1ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Rat anti-Mouse CD155 Monoclonal Antibody | anti-CD155 antibody
Anti-CD155 [3F1]
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, ELISA
Purity
Purified antibody. Protein A affinity purified
Synonyms
CD155; Monoclonal Antibody; Anti-CD155 [3F1]; TAGE 3F1; Poliovirus receptor; cellular receptor for poliovirus; Pvr; Tumor-associated antigen 1; Taa1; Tage4; HVED; anti-CD155 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2a, kappa
Clone Number
3F1
Specificity
This antibody is specific for murine CD155.
Purity/Purification
Purified antibody. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD155 antibody
Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes
The binding specificity of this antibody has been confirmed both in ELISA analysis using recombinant murine CD155 (mCD155), and in flow cytometric analysis using HEK293 cells transiently expressing mCD155 (Maier et al, 2007). This antibody has been used in flow cytometry to assess mCD155 expression on lymphocytes and dendritic cells (Maier et al, 2007; Seth et al, 2011). This antibody blocks the interaction of CD155 with its ligands CD96 and CD226 (Qiu et al, 2010). When administered to WT mice, antibody-induced disruption of the CD155-CD226 interaction has been shown to cause CD226 upregulation on CD4+ T cells and CD8+ T cells (Seth et al, 2011). Administration of this antibody has also been shown to stimulate the loss of mature, memory-like CD8+ T cells from the thymus (Seth et al, 2011; Georgiev et al, 2016).
PMID
17621371
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Western Blot (WB)
(Western Blot using anti-CD155 antibody 3F1 (MBS488666). Mouse thymus lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric rabbit IgG version of 3F1 (MBS488666) at 0.1ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Western Blot (WB)
(Western Blot using anti-CD155 antibody 3F1 (MBS488666). Mouse thymus lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric rabbit IgG version of 3F1 (MBS488666) at 0.1ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Immunofluorescence (IF)
(Immunofluorescence staining of RAW 264.7 cells using anti-CD155 antibody (MBS488666) 3F1. Immunofluorescence analysis of paraformaldehyde fixed RAW 264.7 cells stained with the chimeric rabbit IgG version of 3F1 (MBS488666) at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488666, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (MBS488041) followed by Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of RAW 264.7 cells using anti-CD155 antibody (MBS488666) 3F1. Immunofluorescence analysis of paraformaldehyde fixed RAW 264.7 cells stained with the chimeric rabbit IgG version of 3F1 (MBS488666) at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488666, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (MBS488041) followed by Alexa Fluor 488 secondary antibody.)
ELISA (EIA)
(Binding curve of anti-CD155 antibody 3F1 (MBS488666) to recombinant mouse CD155 Fc-Fusion Protein. ELISA Plate coated with recombinant mouse CD155 Fc-Fusion Protein at a concentration of 5ug/ml. A 3-fold serial dilution from 10,000 ng/ml was performed using MBS488666. For detection, a 1:4000 dilution of HRP-labelled anti-rabbit antibody was used.)
ELISA (EIA)
(Binding curve of anti-CD155 antibody 3F1 (MBS488666) to recombinant mouse CD155 Fc-Fusion Protein. ELISA Plate coated with recombinant mouse CD155 Fc-Fusion Protein at a concentration of 5ug/ml. A 3-fold serial dilution from 10,000 ng/ml was performed using MBS488666. For detection, a 1:4000 dilution of HRP-labelled anti-rabbit antibody was used.)
Related Product Information for anti-CD155 antibody
Recombinant monoclonal antibody to CD155. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 3F1.
This antibody was raised by immunising rats with recombinant murine CD155, consisting of the entire ectodomain expressed as a HIS-tagged protein.
This antibody was raised by immunising rats with recombinant murine CD155, consisting of the entire ectodomain expressed as a HIS-tagged protein.
Product Categories/Family for anti-CD155 antibody
References
Maier et al. The adhesion receptor CD155 determines the magnitude of humoral immune responses against orally ingested antigens Eur J Immunol. 2007 Aug;37(8):2214-25.
Note on publication: Describes the original generation of this antibody, as part of a panel of anti-CD155 antibodies.
Note on publication: Describes the original generation of this antibody, as part of a panel of anti-CD155 antibodies.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
44,668 Da
NCBI Official Full Name
poliovirus receptor
UniProt Protein Name
Poliovirus receptor
UniProt Gene Name
Pvr
UniProt Synonym Gene Names
D7Ertd458e
UniProt Entry Name
Q8K094_MOUSE
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Product Notes
The CD155 pvr (Catalog #AAA488667) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD155 [3F1] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD155 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA). The binding specificity of this antibody has been confirmed both in ELISA analysis using recombinant murine CD155 (mCD155), and in flow cytometric analysis using HEK293 cells transiently expressing mCD155 (Maier et al, 2007). This antibody has been used in flow cytometry to assess mCD155 expression on lymphocytes and dendritic cells (Maier et al, 2007; Seth et al, 2011). This antibody blocks the interaction of CD155 with its ligands CD96 and CD226 (Qiu et al, 2010). When administered to WT mice, antibody-induced disruption of the CD155-CD226 interaction has been shown to cause CD226 upregulation on CD4+ T cells and CD8+ T cells (Seth et al, 2011). Administration of this antibody has also been shown to stimulate the loss of mature, memory-like CD8+ T cells from the thymus (Seth et al, 2011; Georgiev et al, 2016). Researchers should empirically determine the suitability of the CD155 pvr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD155, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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