Mouse anti-Human CD33 Monoclonal Antibody | anti-CD33 antibody

Mouse Anti Human CD33: Amethyst Orange

Cat. Num. AAA225852

• Gene Names: CD33; p67; SIGLEC3; SIGLEC-3
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
• Synonyms: CD33; Monoclonal Antibody; Mouse Anti Human CD33: Amethyst Orange; CD33 antibody; WM53; anti-CD33 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: WM53
• Purity/Purification: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
• Form/Format: Purified IgG conjugated to Amethyst Orange - Liquid
• Concentration: IgG concentration 0.1 mg/ml (varies by lot)
• Sequence Length: 364

• Applicable Applications for anti-CD33 antibody: Flow Cytometry (FC/FACS)
• Application Notes: FC/FACS: Max. Dilution: Neat; Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
• Immunogen: Human AML cells
• Preservative Stabilizers: 0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin
• Fusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line
• Buffer Solution: Phosphate Buffered Saline
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred.; This product should be stored undiluted.; Storage in frost free freezers is not recommended.; This product is photosensitive and should be protected from light.; Avoid repeated freezing and thawing as this may denature the antibody.; This product contains a precipitate we recommend microcentrifugation before use.

Testing Data

(FITC conjugated Mouse anti Human CD14 and Amethyst Orange conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Human CD14 and Amethyst Orange conjugated Mouse anti Human CD33 . All experiments performed on human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)

Testing Data

(Figure A. FITC conjugated mouse anti human CD14 and Alexa647 conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated mouse anti human CD14 and Alexa647 conjugated mouse anti human CD33 . All experiments performed on red cell lysed Human peripheral blood gated on myeloid cells in the presence of Human SeroBlock .)

Testing Data

(Mouse anti Human CD33 antibody, clone WM53 used to evaluate CD33 expression on blood derived NK cells by flow cytometry.Image caption:CD33 expression and function in primary human NK cells. (a) Blood NK cells (CD3-CD56+ lymphocytes in region R1) and (b, c) quiescent primary human NK cells were phenotyped by flow cytometry. Blood CD33+ NK cells were mainly CD56bright (R2 region, dark dots), CD16-, CD57-, NKG2A+, and NKG2D+. The figure shows a representative blood sample out of the four tested.From: Hernández-Caselles T, Miguel RC, Ruiz-Alcaraz AJ, García-Peñarrubia P.CD33 (Siglec-3) Inhibitory Function: Role in the NKG2D/DAP10 Activating Pathway.J Immunol Res. 2019 Apr 15;2019:6032141.doi: 10.1155/2019/6032141.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)

Testing Data

(Mouse anti Human CD33 antibody, clone WM53 used to evaluate CD33 expression on blood derived NK cells using immunoprecipitation.Image caption:CD33 expression and function in primary human NK cells. (d) CD33 coprecipitated SHP-2 phosphatase only in quiescent primary human NK cell lysates. The figure shows a representative experiment out of two with similar results.From: Hernández-Caselles T, Miguel RC, Ruiz-Alcaraz AJ, García-Peñarrubia P.CD33 (Siglec-3) Inhibitory Function: Role in the NKG2D/DAP10 Activating Pathway.J Immunol Res. 2019 Apr 15;2019:6032141.doi: 10.1155/2019/6032141.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)

Testing Data

(Mouse anti Human CD33 antibody, clone WM53 used to evaluate CD33 expression on blood derived quiescent NK cells by flow cytometry.Image caption:CD33 expression and function in primary human NK cells.CD33+ (WM53+ HIM3-4-) quiescent NK cells (R1 region, dark dots) were CD57-, NKG2D+, and partially expressed NKp46 and NKG2A antigens.From: Hernández-Caselles T, Miguel RC, Ruiz-Alcaraz AJ, García-Peñarrubia P.CD33 (Siglec-3) Inhibitory Function: Role in the NKG2D/DAP10 Activating Pathway.J Immunol Res. 2019 Apr 15;2019:6032141.doi: 10.1155/2019/6032141.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)

Related Product Information for anti-CD33 antibody

Mouse anti Human CD33 antibody, clone WM53 recognizes the human CD33 cell surface glycoprotein. This antigen, considered to be specific for the myeloid lineage, has also been reported to be present on cells of lymphoid origin.
Mouse anti Human CD33 antibody, clone WM53 immunoprecipitates a protein of ~75 kDa from myeloid cells, a smaller protein of approximately 67 kDa has been observed in immunoprecipitates from lymphoid targets.

NCBI and Uniprot Product Information

• NCBI GI #: 116241290
• NCBI GeneID: 945
• UniProt Accession #: P20138
• NCBI Official Full Name: Myeloid cell surface antigen CD33
• NCBI Official Synonym Full Names: CD33 molecule
• NCBI Official Symbol: CD33
• NCBI Official Synonym Symbols: p67; SIGLEC3; SIGLEC-3
• NCBI Protein Information: myeloid cell surface antigen CD33
• UniProt Protein Name: Myeloid cell surface antigen CD33
• Protein Family: Myeloid cell surface antigen
• UniProt Gene Name: CD33
• UniProt Synonym Gene Names: SIGLEC3; Siglec-3
• UniProt Entry Name: CD33_HUMAN

Uniprot Description

CD33: Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; receptor activity; carbohydrate binding

Biological Process: negative regulation of cell proliferation; cell-cell signaling; signal transduction; cell adhesion