Testing Data
(Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Amethyst Orange conjugated Rat IgG2b isotype control . Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Amethyst Orange conjugated Rat anti Human CD52 . All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
Rat anti-Human CD52 Monoclonal Antibody | anti-CD52 antibody
Rat Anti Human CD52: Amethyst Orange
Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
This product should be stored undiluted.
Storage in frost free freezers is not recommended.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody.
This product contains a precipitate we recommend microcentrifugation before use.
Testing Data
(Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Amethyst Orange conjugated Rat IgG2b isotype control . Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Amethyst Orange conjugated Rat anti Human CD52 . All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
Testing Data
(Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Amethyst Orange conjugated Rat IgG2b isotype control . Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Amethyst Orange conjugated Rat anti Human CD52 . All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
Testing Data
(Figure A. Pacific Blue conjugated mouse anti human CD3 and RPE conjugated rat IgG2b isotype control . Figure B. Pacific Blue conjugated Mouse anti Human CD3 and RPE conjugated mouse anti human CD52 . All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)
Testing Data
(Figure A. Pacific Blue conjugated mouse anti human CD3 and RPE conjugated rat IgG2b isotype control . Figure B. Pacific Blue conjugated Mouse anti Human CD3 and RPE conjugated mouse anti human CD52 . All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)
Testing Data
(Rat anti Human CD52 antibody, clone YTH34.5 used for staining lymphoma tissue specimens by immunohistochemistry on formalin fixed, paraffin embedded sections.Image caption:Positive CD52 IHC from representative DHL cases reveals a range of staining intensities: 1+ (A), 2+ (B), and 3+ (C). H&E stain of the strongly CD52-positive DHL case depicted in C (D). Many cases exhibited uniform staining intensity (E), while others showed variable CD52 expression (F).From: Craig JW, Mina MJ, Crombie JL, LaCasce AS, Weinstock DM, Pinkus GS, et al. (2018)Assessment of CD52 expression in "double-hit" and "double-expressor" lymphomas: Implications for clinical trial eligibility.PLoS ONE 13(7): e0199708.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Testing Data
(Rat anti Human CD52 antibody, clone YTH34.5 used for staining lymphoma tissue specimens by immunohistochemistry on formalin fixed, paraffin embedded sections.Image caption:Positive CD52 IHC from representative DHL cases reveals a range of staining intensities: 1+ (A), 2+ (B), and 3+ (C). H&E stain of the strongly CD52-positive DHL case depicted in C (D). Many cases exhibited uniform staining intensity (E), while others showed variable CD52 expression (F).From: Craig JW, Mina MJ, Crombie JL, LaCasce AS, Weinstock DM, Pinkus GS, et al. (2018)Assessment of CD52 expression in "double-hit" and "double-expressor" lymphomas: Implications for clinical trial eligibility.PLoS ONE 13(7): e0199708.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Testing Data
(Figure A. FITC conjugated Mouse anti Human CD52 antibody, clone YTH34.5 used for the evaluation of CD52 expression on human cell lines by flow cytometry.Image caption:WM-specific antigen expression compared across RPCI-WM1, BCWM.1 and MWCL-1 cell lines.Fluorescein (FITC), phycoerythrin (PE), phycoerythrin—cyanine 5 (PC5) or allophycocyanin (APC) conjugates of various antibodies were used as presented above. All cell lines were negative for CD10 and 11c. Blue line indicates RPCI-WM1 antigen expression, red line indicates MWCL-1 antigen expression and green line indicates antigen expression in BCWM.1 cell line. Only BCWM.1 an MWCL-1 were CD19+, CD20+ and FMC7+. Expression of CD138 and ?-light-chain was seen only on MWCL-1 and RPCI-WM1 cells. CD28 expression was markedly more observable on RPCI-WM1 tumor cells as compared to MWCL-1 or BCWM.1.From: Paulus A, Chitta KS, Wallace PK, Advani PP, Akhtar S, Kuranz-Blake M, et al. (2015)Immunophenotyping of Waldenströms Macroglobulinemia Cell Lines Reveals Distinct Patterns of Surface Antigen Expression: Potential Biological and Therapeutic Implications.PLoS ONE 10(4): e0122338.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Testing Data
(Figure A. FITC conjugated Mouse anti Human CD52 antibody, clone YTH34.5 used for the evaluation of CD52 expression on human cell lines by flow cytometry.Image caption:WM-specific antigen expression compared across RPCI-WM1, BCWM.1 and MWCL-1 cell lines.Fluorescein (FITC), phycoerythrin (PE), phycoerythrin—cyanine 5 (PC5) or allophycocyanin (APC) conjugates of various antibodies were used as presented above. All cell lines were negative for CD10 and 11c. Blue line indicates RPCI-WM1 antigen expression, red line indicates MWCL-1 antigen expression and green line indicates antigen expression in BCWM.1 cell line. Only BCWM.1 an MWCL-1 were CD19+, CD20+ and FMC7+. Expression of CD138 and ?-light-chain was seen only on MWCL-1 and RPCI-WM1 cells. CD28 expression was markedly more observable on RPCI-WM1 tumor cells as compared to MWCL-1 or BCWM.1.From: Paulus A, Chitta KS, Wallace PK, Advani PP, Akhtar S, Kuranz-Blake M, et al. (2015)Immunophenotyping of Waldenströms Macroglobulinemia Cell Lines Reveals Distinct Patterns of Surface Antigen Expression: Potential Biological and Therapeutic Implications.PLoS ONE 10(4): e0122338.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Testing Data
(FITC Conjugated Mouse anti Human CD52 antibody, clone YTH34.5 used for the evaluation of CD52 expression on RPCI-WM1 cells by flow cytometry.Image caption:Expression of WM-specific antigens present on RPCI-WM1.For antigen detection, fluorescein (FITC), phycoerythrin (PE), phycoerythrin—cyanine 5 (PC5) or allophycocyanin (APC) conjugates of various antigen-specific antibodies were used. Flow cytometry shows the RPCI-WM1 cell line to be CD10, 11c, 19, 20, 23, 27, 40, 52 and 79b negative and CD22, 28, 38, 45, 45RO, 38, 138, ? light chain and intracellular-? positive.From: Paulus A, Chitta KS, Wallace PK, Advani PP, Akhtar S, Kuranz-Blake M, et al. (2015)Immunophenotyping of Waldenströms Macroglobulinemia Cell Lines Reveals Distinct Patterns of Surface Antigen Expression: Potential Biological and Therapeutic Implications.PLoS ONE 10(4): e0122338.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Testing Data
(FITC Conjugated Mouse anti Human CD52 antibody, clone YTH34.5 used for the evaluation of CD52 expression on RPCI-WM1 cells by flow cytometry.Image caption:Expression of WM-specific antigens present on RPCI-WM1.For antigen detection, fluorescein (FITC), phycoerythrin (PE), phycoerythrin—cyanine 5 (PC5) or allophycocyanin (APC) conjugates of various antigen-specific antibodies were used. Flow cytometry shows the RPCI-WM1 cell line to be CD10, 11c, 19, 20, 23, 27, 40, 52 and 79b negative and CD22, 28, 38, 45, 45RO, 38, 138, ? light chain and intracellular-? positive.From: Paulus A, Chitta KS, Wallace PK, Advani PP, Akhtar S, Kuranz-Blake M, et al. (2015)Immunophenotyping of Waldenströms Macroglobulinemia Cell Lines Reveals Distinct Patterns of Surface Antigen Expression: Potential Biological and Therapeutic Implications.PLoS ONE 10(4): e0122338.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
The apparent molecular mass of the native antigen on SDS-PAGE is 25-29 kDa, considerably reduced following N-glycanase treatment (Rowan et al. 1998).
CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.
Humanized versions of CAMPATH-1 specific antibodies are currently in clinical trials for the treatment of a range of lymphoid malignancies (Dearden et al. 2002; Pettitt et al. 2012).
NCBI and Uniprot Product Information
Uniprot Description
May play a role in carrying and orienting carbohydrate, as well as having a more specific role.
Research Articles on CD52
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Product Notes
The CD52 cd52 (Catalog #AAA225858) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti Human CD52: Amethyst Orange reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD52 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: Min. Dilution: Neat, Max. Dilution: 1/5 Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul. Researchers should empirically determine the suitability of the CD52 cd52 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD52, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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