Mouse anti-Human CD45 Monoclonal Antibody | anti-CD45 antibody

Mouse Anti Human CD45: Amethyst Orange

Cat. Num. AAA225855

• Gene Names: PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
• Synonyms: CD45; Monoclonal Antibody; Mouse Anti Human CD45: Amethyst Orange; CD45 antibody; F10-89-4; anti-CD45 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: F10-89-4
• Purity/Purification: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
• Form/Format: Purified IgG conjugated to Amethyst Orange - Liquid
• Concentration: IgG concentration 0.1 mg/ml (varies by lot)
• Sequence Length: 1306

• Applicable Applications for anti-CD45 antibody: Flow Cytometry (FC/FACS)
• Application Notes: FC/FACS: Min. Dilution: Neat, Max. Dilution: 1/10; Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
• Immunogen: Human T lymphocytes.
• Buffer Solution: Phosphate Buffered Saline
• Preservative Stabilizers: 0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin
• Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred.; This product should be stored undiluted.; Storage in frost free freezers is not recommended.; This product is photosensitive and should be protected from light.; Avoid repeated freezing and thawing as this may denature the antibody.; This product contains a precipitate we recommend microcentrifugation before use.

Testing Data

(Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD4 and Alexa Fluor 647 conjugated Mouse IgG2a isotype control . Figure B . Alexa Fluor 488 conjugated Mouse anti Human CD4 and Alexa Fluor 647 conjugated Mouse anti Human CD45 . All experiments performed on Human blood gated on live single cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)

Testing Data

(Figure A. FITC conjugated Mouse anti Human CD19 and Alexa Fluor 647 conjugated Mouse IgG2a isotype control . Figure B. FITC conjugated Mouse anti Human CD19 and Alexa Fluor 647 conjugated Mouse anti Human CD45 . All experiments performed on red cell lysed human peripheral blood gated on leukocytes in the presence of Human SeroBlock .)

Testing Data

(Direction of Mouse anti Human CD45 in an immunohistochemical analysis of an acetone fixed, frozen human colon section using the Histar Detection Kit .)

Testing Data

(Mouse anti Human CD45 antibody, clone F10-89-4 used for the evaluation of CD45 expression on brain derived mesenchymal stem cells.Image caption:FACS sorting of brain-derived progenitor cell lines. Human progenitor cells were positively sorted for the MSC markers CD105 and CD13, and thereafter negatively for hematopoietic (CD45) and endothelial markers (CD31). Histogram illustrates results (A, B) for the two different cortical lines and (C, D) for the two lines from the ventricular zone. 99.4–100% of the cell population expressed both, CD105 and CD13, and cultures did not contain endothelial or hematopoietic cells.From: Paul G, Özen I, Christophersen NS, Reinbothe T, Bengzon J, et al. (2012) The Adult Human Brain Harbors Multipotent Perivascular Mesenchymal Stem Cells.PLoS ONE 7(4): e35577.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)

Testing Data

(Mouse anti Human CD45 antibody, clone F10-89-4 used for the evaluation of CD45 expression on brain derived mesenchymal stem cells.Image caption:Brain-derived cell lines express markers for mesenchymal stem cells and pericytes but not for glial or neuronal precursors. (A) Representative histogram of flow cytometry analysis of cortical line and (B) ventricular zone line. Progenitors from all donors and both regions highly express MSC (CD90, CD73, CD105, CD29, CD166 and CD49d) and pericyte markers (CD140b/PDGFR-?, RGS5, CD146, Nestin, ?-SMA and NG2). They do not express hematopoietic (CD45), endothelial (CD31, CD34), microglial (CD14, CD11), glial or neuronal precursor cell markers (GFAP, O4) or myofibroblast markers (CD56), (green = isotype, red = respective marker).From: Paul G, Özen I, Christophersen NS, Reinbothe T, Bengzon J, et al. (2012) The Adult Human Brain Harbors Multipotent Perivascular Mesenchymal Stem Cells.PLoS ONE 7(4): e35577.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)

Testing Data

(Mouse anti Human CD45 antibody, clone F10-89-4 used for the evaluation of CD45 expression on brain derived mesenchymal stem cells.Image caption:Characterisation of clonal perivascular MSC progenitor lines. (A) Single cell in vitro a few hours after plating, and culture at 7 and 21 days, respectively. Scale bar 50 µm. (B) Clonally derived MSC show the same antigen profile as detected for polyclonal lines with pericyte and MSC markers but neither neuronal nor glial precursor cell markers (O4, GFAP) (example here cortical clone; green = isotype, red = respective marker).(C) QPCR comparing clones derived from the ventricular zone (n = 2), and the cortex (n = 2) to a human neuronal progenitor cell line MesC2.10. Data show the relative higher expression of mRNA for RGS5, PDGFR-? and ?-SMA in the MSC clones compared to the control. As expected, Nestin mRNA was present in perivascular MSC clones and neural progenitors at comparable amounts. NG2 mRNA was slightly higher expressed in the MSC clones compared to the control. (D) Importantly, neural progenitors markers such as CD133, SOX1, NGN2, PAX6 and Musashi were highly expressed in the positive control but not detectable in MSC clones.From: Paul G, Özen I, Christophersen NS, Reinbothe T, Bengzon J, et al. (2012)The Adult Human Brain Harbors Multipotent Perivascular Mesenchymal Stem Cells.PLoS ONE 7(4): e35577.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)

Testing Data

(Alexa Fluor A647 conjugated Mouse anti Human CD45 antibody, clone F10-89-4 used for the identification of CD45 expressing cells in blood samples by immunofluorescence.Image caption:Relocation and characterization of CTCs using the Wright-Giemsa stain. After initial identification of CTCs via fluorescent images, subsequent Wright-Giemsa stain and/or Papanicolau stain of these cells is performed: (a) composite 3-color fluorescent image, (b) DAPI channel only, (c) cytokeratin channel only, (d) CD45 channel only, and (e) corresponding Wright-Giemsa-stained cell.From: Marrinucci D, Bethel K, Lazar D, Fisher J, Huynh E, Clark P, Bruce R, Nieva J, Kuhn P.Cytomorphology of circulating colorectal tumor cells: a small case series.J Oncol. 2010:861341.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)

Testing Data

(Alexa Fluor A647 conjugated Mouse anti Human CD45 antibody, clone F10-89-4 used for the identification of CD45 expressing cells in blood samples by immunofluorescence.Image caption:Representative CTCs found in Patients 1–5. Each column represents one patient's CTCs: Red = cytokeratin, Green = CD45, and Blue = DAPI.From: Marrinucci D, Bethel K, Lazar D, Fisher J, Huynh E, Clark P, Bruce R, Nieva J, Kuhn P.Cytomorphology of circulating colorectal tumor cells: a small case series.J Oncol. 2010:861341.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)

Testing Data

(FFPE human tonsil stained with Mouse Anti-Human CD45 , biotinylated Anti-Mouse IgG and Streptavidin Alkaline Phosphatase (red). Nuclei were counterstained with hematoxylin.)

Testing Data

(Phycoerythrin conjugated Mouse anti Human CD45 antibody, clone F10-89-4 used to evaluate CD45 expression on bone-marrow derived mesenchymal stem cells by flow cytometryImage caption:From: Chaturvedi CP, Tripathy NK, Minocha E, Sharma A, Rahman K, Nityanand S.Altered Expression of Hematopoiesis Regulatory Molecules in Lipopolysaccharide-Induced Bone Marrow Mesenchymal Stem Cells of Patients with Aplastic Anemia.Stem Cells Int. 2018 Oct 17;2018:6901761.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)

Related Product Information for anti-CD45 antibody

Mouse anti Human CD45 antibody, clone F10-89-4 recognizes the human CD45 cell surface antigen, also known as the leucocyte common antigen (LCA). CD45 is a complex molecule existing in a number of isoforms.
Antibodies recognising a common epitope on all of these isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
Mouse anti Human CD45 antibody, clone F10-89-4 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes.
Mouse anti Human CD45 antibody, clone F10-89-4 is routinely tested in flow cytometry on human peripheral blood leucocytes

NCBI and Uniprot Product Information

• NCBI GI #: 392307007
• NCBI GeneID: 5788
• NCBI Accession #: NP_002829.3
• NCBI GenBank Nucleotide #: NP_002829.3
• UniProt Accession #: P08575
• NCBI Official Full Name: receptor-type tyrosine-protein phosphatase C isoform 1
• NCBI Official Synonym Full Names: protein tyrosine phosphatase receptor type C
• NCBI Official Symbol: PTPRC
• NCBI Official Synonym Symbols: LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
• NCBI Protein Information: receptor-type tyrosine-protein phosphatase C
• UniProt Protein Name: Receptor-type tyrosine-protein phosphatase C
• UniProt Gene Name: PTPRC
• UniProt Synonym Gene Names: CD45; L-CA
• UniProt Entry Name: PTPRC_HUMAN

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]

Uniprot Description

CD45: a receptor type protein tyrosine phosphatase protein. Contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. The first PTPAse domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Specifically expressed in hematopoietic cells. An essential regulator of T- and B-cell antigen receptor signaling. Functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. Suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced isoforms have been described.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Receptor protein phosphatase, tyrosine; EC 3.1.3.48

Chromosomal Location of Human Ortholog: 1q31-q32

Cellular Component: focal adhesion; membrane; integral to plasma membrane; plasma membrane; lipid raft; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor protein tyrosine phosphatase activity; protein tyrosine phosphatase activity; protein kinase binding

Biological Process: B cell proliferation; axon guidance; substrate-bound cell migration, cell release from substrate; regulation of cell cycle; protein amino acid dephosphorylation; positive regulation of antigen receptor-mediated signaling pathway; T cell receptor signaling pathway; bone marrow development; B cell receptor signaling pathway; stem cell development; cell surface receptor linked signal transduction; dephosphorylation; release of sequestered calcium ion into cytosol; positive regulation of protein kinase activity; negative regulation of T cell mediated cytotoxicity; positive regulation of B cell proliferation; negative regulation of protein kinase activity; positive regulation of T cell proliferation; hemopoietic progenitor cell differentiation; negative regulation of cytokine and chemokine mediated signaling pathway; immunoglobulin biosynthetic process; defense response to virus; T cell differentiation

Disease: Hepatitis C Virus, Susceptibility To; Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-positive, Nk Cell-positive