Testing Data
(Flow Cytometric staining of Jurkat cells with preservative free Mouse anti Human CD166 antibody, clone 3A6 , filled histogram, with Mouse IgG isotype control , open histogram.)
Mouse anti-Human, Sheep CD166 Monoclonal Antibody | anti-CD166 antibody
Mouse Anti Human CD166: RPE
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Testing Data
(Flow Cytometric staining of Jurkat cells with preservative free Mouse anti Human CD166 antibody, clone 3A6 , filled histogram, with Mouse IgG isotype control , open histogram.)
Testing Data
(Flow Cytometric staining of Jurkat cells with preservative free Mouse anti Human CD166 antibody, clone 3A6 , filled histogram, with Mouse IgG isotype control , open histogram.)
Flow Cytometry (FC/FACS)
(Flow Cytometric staining of Jurkat cells with FITC conjugated Mouse anti Human CD166 antibody, clone 3A6 , filled histogram and FITC conjugated Mouse IgG1 isotype control , open histogram.)
Flow Cytometry (FC/FACS)
(Flow Cytometric staining of Jurkat cells with FITC conjugated Mouse anti Human CD166 antibody, clone 3A6 , filled histogram and FITC conjugated Mouse IgG1 isotype control , open histogram.)
Testing Data
(Mouse Anti Human CD166 antibody, clone 3A6 used for the evaluation of CD166 expression on umbilical cord derived mesenchymal stem cells by flow cytometry.Image caption:Flow cytometry, Immunofluorescence and qRT-PCR of WJ-MSCs. (A). Representative flow cytometry of WJ-MSCs (n = 3). Cells express CD29, CD44, CD73, CD90, CD105, and are negative for the hematopoietic (CD34 and CD45) and endothelial (CD106 and CD133) markers. Black open histogram indicates controls signal; red shaded histogram represents positive reactivity with the indicated antibody. (B) Confocal laser images of Immunofluorescence using APEX-labeling system for conjugating primary antibodies; CD29-Alexa Fluor 594, CD34-, CD44-, CD90- and CD133- Alexa Fluor 488. CD73-PE and CD105-PE were manufacturer labeled. 600X magnifications (C) qRT-PCR of the prospective markers for RNA isolated from undifferentiated WJ-MSCs cells, values were expressed as a percentage relative to 1/dCt of GAPDH gene.From: Ali H, Al-Yatama MK, Abu-Farha M, Behbehani K, Al Madhoun A (2015)Multi-Lineage Differentiation of Human Umbilical Cord Wharton's Jelly Mesenchymal Stromal Cells Mediates Changes in the Expression Profile of Stemness Markers.PLoS ONE 10(4): e0122465.This is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Testing Data
(Mouse Anti Human CD166 antibody, clone 3A6 used for the evaluation of CD166 expression on umbilical cord derived mesenchymal stem cells by flow cytometry.Image caption:Flow cytometry, Immunofluorescence and qRT-PCR of WJ-MSCs. (A). Representative flow cytometry of WJ-MSCs (n = 3). Cells express CD29, CD44, CD73, CD90, CD105, and are negative for the hematopoietic (CD34 and CD45) and endothelial (CD106 and CD133) markers. Black open histogram indicates controls signal; red shaded histogram represents positive reactivity with the indicated antibody. (B) Confocal laser images of Immunofluorescence using APEX-labeling system for conjugating primary antibodies; CD29-Alexa Fluor 594, CD34-, CD44-, CD90- and CD133- Alexa Fluor 488. CD73-PE and CD105-PE were manufacturer labeled. 600X magnifications (C) qRT-PCR of the prospective markers for RNA isolated from undifferentiated WJ-MSCs cells, values were expressed as a percentage relative to 1/dCt of GAPDH gene.From: Ali H, Al-Yatama MK, Abu-Farha M, Behbehani K, Al Madhoun A (2015)Multi-Lineage Differentiation of Human Umbilical Cord Wharton's Jelly Mesenchymal Stromal Cells Mediates Changes in the Expression Profile of Stemness Markers.PLoS ONE 10(4): e0122465.This is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Testing Data
(Published Customer image:Mouse Anti Human CD166 antibody, clone 3A6 used for the evaluation of CD166 expression on mesenchymal stromal cells by flow cytometry.Image caption:Characterization and differentiation of BM-MSC. (A) Representative flow cytometric analysis of cultured mesenchymal stromal cells. Solid white represents the isotype control. (B) A. BM-MSC, displayed an homogeneous morphology of fibroblastic cells. Cells were stained with May Grunwald Giemsa staining (40×). Under specific induction BM-MSC were differentiated into (B) adipocytes (lipid vacuoles were colored by Oil Red O, ×40), (C) osteocytes (calcium deposits were revealed by Von Kossa method, ×40), (D) chondrocytes (cell pellet was sectioned and stained by toluidine blue, ×4), and (E) neuron-like cells derived from BM-MSC upon treatment with neurogenic medium (×40). Expression of nestin and MAP-2 after 10 days induction, detected by immunofluorescence (F and G, 100×).From: Tondreau T, Dejeneffe M, Meuleman N, Stamatopoulos B, Delforge A, Martiat P, Bron D, Lagneaux L. Gene expression pattern of functional neuronal cells derived from human bone marrow mesenchymal stromal cells.BMC Genomics. 2008 Apr 11;9:166.This is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Testing Data
(Published Customer image:Mouse Anti Human CD166 antibody, clone 3A6 used for the evaluation of CD166 expression on mesenchymal stromal cells by flow cytometry.Image caption:Characterization and differentiation of BM-MSC. (A) Representative flow cytometric analysis of cultured mesenchymal stromal cells. Solid white represents the isotype control. (B) A. BM-MSC, displayed an homogeneous morphology of fibroblastic cells. Cells were stained with May Grunwald Giemsa staining (40×). Under specific induction BM-MSC were differentiated into (B) adipocytes (lipid vacuoles were colored by Oil Red O, ×40), (C) osteocytes (calcium deposits were revealed by Von Kossa method, ×40), (D) chondrocytes (cell pellet was sectioned and stained by toluidine blue, ×4), and (E) neuron-like cells derived from BM-MSC upon treatment with neurogenic medium (×40). Expression of nestin and MAP-2 after 10 days induction, detected by immunofluorescence (F and G, 100×).From: Tondreau T, Dejeneffe M, Meuleman N, Stamatopoulos B, Delforge A, Martiat P, Bron D, Lagneaux L. Gene expression pattern of functional neuronal cells derived from human bone marrow mesenchymal stromal cells.BMC Genomics. 2008 Apr 11;9:166.This is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Testing Data
(Alexa Fluor 488 conjugated Mouse anti Human CD166 antibody, clone 3A6 used for the evaluation of ALCAM expression on human breast epithelial cells (HBECs) by flow cytometry.Image caption:ERpos cells are purified and tracked by sequential CD326/CD271-CD166/CD117 FACS followed by multicolour staining and qRT-PCR.Multicolour flow cytometry of uncultured HBECs incubated with CD326/CD271/CD166/CD117 and visualized pairwise (left diagrams) to recover luminal cells (CD326high) and basal cells (CD271high) and from the luminal gate CD166high and CD117high cells. Smears of sorted cells were stained (right panel) with either of the markers against basal cells, cytokeratin K14; luminal cells, cytokeratin K18; luminal progenitors, cytokeratin K15; Ks20.8 or ER-PR and counterstained with DAPI nuclear stain. Hormone receptor-positive cells are observed primarily among CD166high cells. Scale bar, 50?mum. (b) Purity of sorted cells as determined by staining of smears followed by quantification of the percentage of cells stained with either of the markers cytokeratin K14, K18, K15, Ks20.8 or hormone receptors (ER-PR; 3 × 100 cells per slide, error bars indicate s.d.'s). (c) Heatmap representing qRT-PCR analysis of the relative gene expression of lineage markers in sorted basal cells (basal), CD117high luminal cells (CD117) and CD166high luminal cells (CD166) from six different biopsies. Data confirm lineage-specific transcriptional profiles of the three cell populations and restricts ER expression (ESR1) primarily to CD166high luminal cells. Colour bar indicates the fold difference of the relative gene expression in log2 scale.From: Fridriksdottir AJ, Kim J, Villadsen R, Klitgaard MC, Hopkinson BM, Petersen OW, Rønnov-Jessen L.Propagation of oestrogen receptor-positive and oestrogen-responsive normal human breast cells in culture.Nat Commun. 2015 Nov 13;6:8786.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Testing Data
(Alexa Fluor 488 conjugated Mouse anti Human CD166 antibody, clone 3A6 used for the evaluation of ALCAM expression on human breast epithelial cells (HBECs) by flow cytometry.Image caption:ERpos cells are purified and tracked by sequential CD326/CD271-CD166/CD117 FACS followed by multicolour staining and qRT-PCR.Multicolour flow cytometry of uncultured HBECs incubated with CD326/CD271/CD166/CD117 and visualized pairwise (left diagrams) to recover luminal cells (CD326high) and basal cells (CD271high) and from the luminal gate CD166high and CD117high cells. Smears of sorted cells were stained (right panel) with either of the markers against basal cells, cytokeratin K14; luminal cells, cytokeratin K18; luminal progenitors, cytokeratin K15; Ks20.8 or ER-PR and counterstained with DAPI nuclear stain. Hormone receptor-positive cells are observed primarily among CD166high cells. Scale bar, 50?mum. (b) Purity of sorted cells as determined by staining of smears followed by quantification of the percentage of cells stained with either of the markers cytokeratin K14, K18, K15, Ks20.8 or hormone receptors (ER-PR; 3 × 100 cells per slide, error bars indicate s.d.'s). (c) Heatmap representing qRT-PCR analysis of the relative gene expression of lineage markers in sorted basal cells (basal), CD117high luminal cells (CD117) and CD166high luminal cells (CD166) from six different biopsies. Data confirm lineage-specific transcriptional profiles of the three cell populations and restricts ER expression (ESR1) primarily to CD166high luminal cells. Colour bar indicates the fold difference of the relative gene expression in log2 scale.From: Fridriksdottir AJ, Kim J, Villadsen R, Klitgaard MC, Hopkinson BM, Petersen OW, Rønnov-Jessen L.Propagation of oestrogen receptor-positive and oestrogen-responsive normal human breast cells in culture.Nat Commun. 2015 Nov 13;6:8786.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Mouse anti Human CD166 antibody, clone 3A6 is reported to cross-react with CD166 on ovine tissues and provides a useful tool for the identification and characterization of ovine mesenchymal stem cells in conjunction with CD44 which is expressed by this cell lineage and the hematopoietic cell marker CD45 which is not expressed on mesenchymal stem cells (Sanjurjo-Rodríguez et al. 2017).
NCBI and Uniprot Product Information
NCBI Description
This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2011]
Uniprot Description
ALCAM: Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Cell adhesion
Chromosomal Location of Human Ortholog: 3q13.1
Cellular Component: focal adhesion; cell soma; axon; integral to membrane; external side of plasma membrane
Molecular Function: protein binding; receptor binding
Biological Process: axon guidance; motor axon guidance; signal transduction; cell adhesion
Research Articles on CD166
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Product Notes
The CD166 alcam (Catalog #AAA225638) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti Human CD166: RPE reacts with Human, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's CD166 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the CD166 alcam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD166, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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