Testing Data
(Figure A. RPE conjugated mouse anti human CD3 and Alexa 647 conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated mouse anti human CD3 and Alexa 647 conjugated mouse anti human CD16 . All experiments performed on red cell lysed human peripheral blood gated on lymphocytes in the presence of Human SeroBlock .)
Mouse anti-Human CD16 Monoclonal Antibody | anti-CD16 antibody
Mouse Anti Human CD16: Amethyst Orange
This product should be stored undiluted.
Storage in frost free freezers is not recommended.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody.
This product contains a precipitate we recommend microcentrifugation before use.
Testing Data
(Figure A. RPE conjugated mouse anti human CD3 and Alexa 647 conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated mouse anti human CD3 and Alexa 647 conjugated mouse anti human CD16 . All experiments performed on red cell lysed human peripheral blood gated on lymphocytes in the presence of Human SeroBlock .)
Testing Data
(Figure A. RPE conjugated mouse anti human CD3 and Alexa 647 conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated mouse anti human CD3 and Alexa 647 conjugated mouse anti human CD16 . All experiments performed on red cell lysed human peripheral blood gated on lymphocytes in the presence of Human SeroBlock .)
Testing Data
(Figure A. Alexa 488 conjugated mouse anti human CD56 and Alexa 647 conjugated Mouse IgG1 isotype control . Figure B. Alexa 488 conjugated mouse anti human CD56 and Alexa 647 conjugated mouse anti human CD16 . All experiments performed on red cell lysed human peripheral blood gated on lymphocytes in the presence of Human SeroBlock .)
Testing Data
(Figure A. Alexa 488 conjugated mouse anti human CD56 and Alexa 647 conjugated Mouse IgG1 isotype control . Figure B. Alexa 488 conjugated mouse anti human CD56 and Alexa 647 conjugated mouse anti human CD16 . All experiments performed on red cell lysed human peripheral blood gated on lymphocytes in the presence of Human SeroBlock .)
Testing Data
(Mouse anti Human CD16 antibody, clone LNK16 used as a blocking agent to assess the effect of Fc?Rs on heparin binding protein (HBP) release by ELISA.Image caption:Antibodies against Fc?RII inhibit HBP release.Whole blood (diluted 1?10) was incubated with antibodies against different Fc?Rs, isotype control antibodies, or buffer alone, for 20 minutes at 37°C. M1 protein was added and the HBP release was measured. Data are expressed as percentage of release without any antibodies added, and are the mean±SD from three different individuals, each done in duplicate. The different treatment groups were compared using ANOVA, and when found significantly different the treatment groups were compared against the control group using Dunnett's 2 sided test.From: Kahn F, Mörgelin M, Shannon O, Norrby-Teglund A, Herwald H, Olin AI, et al. (2008) Antibodies against a Surface Protein of Streptococcus pyogenes Promote a Pathological Inflammatory Response. PLoS Pathog 4(9): e1000149.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Testing Data
(Mouse anti Human CD16 antibody, clone LNK16 used as a blocking agent to assess the effect of Fc?Rs on heparin binding protein (HBP) release by ELISA.Image caption:Antibodies against Fc?RII inhibit HBP release.Whole blood (diluted 1?10) was incubated with antibodies against different Fc?Rs, isotype control antibodies, or buffer alone, for 20 minutes at 37°C. M1 protein was added and the HBP release was measured. Data are expressed as percentage of release without any antibodies added, and are the mean±SD from three different individuals, each done in duplicate. The different treatment groups were compared using ANOVA, and when found significantly different the treatment groups were compared against the control group using Dunnett's 2 sided test.From: Kahn F, Mörgelin M, Shannon O, Norrby-Teglund A, Herwald H, Olin AI, et al. (2008) Antibodies against a Surface Protein of Streptococcus pyogenes Promote a Pathological Inflammatory Response. PLoS Pathog 4(9): e1000149.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Testing Data
(Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD56 and Amethyst Orange conjugated Mouse IgG1 isotype control . Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD56 and Amethyst Orange conjugated Mouse anti Human CD16 . All experiments performed on human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
Testing Data
(Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD56 and Amethyst Orange conjugated Mouse IgG1 isotype control . Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD56 and Amethyst Orange conjugated Mouse anti Human CD16 . All experiments performed on human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC by NK cells. Mouse anti Human CD16 antibody, clone LNK16 has been reported to block the binding of IgG to the CD16 molecule (Tamm and Schmidt 1996).
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Research Articles on CD16
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Product Notes
The CD16 (Catalog #AAA225847) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti Human CD16: Amethyst Orange reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD16 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: Min. Dilution: Neat. Researchers should empirically determine the suitability of the CD16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD16, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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