Testing Data
(Figure A. FITC conjugated Mouse anti Human CD14 and Alexa Fluor 700 conjugated Mouse IgG1 isotype control . Figure B FITC conjugated Mouse anti Human CD14 and Alexa Fluor 700 conjugated Mouse anti Human CD64 . All experiments performed on Human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
Mouse anti-Human CD64 Monoclonal Antibody | anti-CD64 antibody
Mouse Anti Human CD64: Amethyst Orange
Use 10ul of the suggested working dilution to label 106 cells in 100ul
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody.
This product contains a precipitate we recommend microcentrifugation before use
Testing Data
(Figure A. FITC conjugated Mouse anti Human CD14 and Alexa Fluor 700 conjugated Mouse IgG1 isotype control . Figure B FITC conjugated Mouse anti Human CD14 and Alexa Fluor 700 conjugated Mouse anti Human CD64 . All experiments performed on Human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
Testing Data
(Figure A. FITC conjugated Mouse anti Human CD14 and Alexa Fluor 700 conjugated Mouse IgG1 isotype control . Figure B FITC conjugated Mouse anti Human CD14 and Alexa Fluor 700 conjugated Mouse anti Human CD64 . All experiments performed on Human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
Testing Data
(Figure A. Alexa Fluor700 conjugated mouse anti human CD14 and Alexa Fluor488 conjugated mouse IgG1 isotype control . Figure B. Alexa Fluor700 conjugated mouse anti human CD14 and Alexa Fluor488 conjugated mouse anti human CD64 . All experiments performed on red cell lysed human blood gated on mononuclear cells in the presence of Human Seroblock . Data acquired on the ZE5 cell analyzer.)
Testing Data
(Figure A. Alexa Fluor700 conjugated mouse anti human CD14 and Alexa Fluor488 conjugated mouse IgG1 isotype control . Figure B. Alexa Fluor700 conjugated mouse anti human CD14 and Alexa Fluor488 conjugated mouse anti human CD64 . All experiments performed on red cell lysed human blood gated on mononuclear cells in the presence of Human Seroblock . Data acquired on the ZE5 cell analyzer.)
Testing Data
(Mouse anti Human CD64 antibody, clone 10.1 used as a blocking agent to assess the effect of Fc?Rs on heparin binding protein (HBP) release by ELISA.Image caption:Antibodies against Fc?RII inhibit HBP release.Whole blood (diluted 1?10) was incubated with antibodies against different Fc?Rs, isotype control antibodies, or buffer alone, for 20 minutes at 37°C. M1 protein was added and the HBP release was measured. Data are expressed as percentage of release without any antibodies added, and are the mean±SD from three different individuals, each done in duplicate. The different treatment groups were compared using ANOVA, and when found significantly different the treatment groups were compared against the control group using Dunnett's 2 sided test.From: Kahn F, Mörgelin M, Shannon O, Norrby-Teglund A, Herwald H, Olin AI, et al. (2008) Antibodies against a Surface Protein of Streptococcus pyogenes Promote a Pathological Inflammatory Response. PLoS Pathog 4(9): e1000149. .This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Testing Data
(Mouse anti Human CD64 antibody, clone 10.1 used as a blocking agent to assess the effect of Fc?Rs on heparin binding protein (HBP) release by ELISA.Image caption:Antibodies against Fc?RII inhibit HBP release.Whole blood (diluted 1?10) was incubated with antibodies against different Fc?Rs, isotype control antibodies, or buffer alone, for 20 minutes at 37°C. M1 protein was added and the HBP release was measured. Data are expressed as percentage of release without any antibodies added, and are the mean±SD from three different individuals, each done in duplicate. The different treatment groups were compared using ANOVA, and when found significantly different the treatment groups were compared against the control group using Dunnett's 2 sided test.From: Kahn F, Mörgelin M, Shannon O, Norrby-Teglund A, Herwald H, Olin AI, et al. (2008) Antibodies against a Surface Protein of Streptococcus pyogenes Promote a Pathological Inflammatory Response. PLoS Pathog 4(9): e1000149. .This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Testing Data
(Figure A. FITC conjugated Mouse anti Human CD14 and Pacific Blue conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Human CD14 and Pacific Blue conjugated Mouse anti Human CD64 . All experiments performed on human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)
Testing Data
(Figure A. FITC conjugated Mouse anti Human CD14 and Pacific Blue conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Human CD14 and Pacific Blue conjugated Mouse anti Human CD64 . All experiments performed on human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)
Testing Data
(Figure A. PE conjugated Mouse anti Human CD14 and Amethyst Orange conjugated Mouse IgG1 isotype control . Figure B. PE conjugated Mouse anti Human CD14 and Amethyst Orange conjugated Mouse anti Human CD64 . All experiments performed on human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
Testing Data
(Figure A. PE conjugated Mouse anti Human CD14 and Amethyst Orange conjugated Mouse IgG1 isotype control . Figure B. PE conjugated Mouse anti Human CD14 and Amethyst Orange conjugated Mouse anti Human CD64 . All experiments performed on human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
Mouse anti Human CD64 antibody, clone 10.1 blocks binding of immunoglobulin to FcRI.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that plays an important role in the immune response. This protein is a high-affinity Fc-gamma receptor. The gene is one of three related gene family members located on chromosome 1. [provided by RefSeq, Jul 2008]
Uniprot Description
FCGR1A: High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. Belongs to the immunoglobulin superfamily. FCGR1 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Receptor, misc.; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1q21.2-q21.3
Cellular Component: early endosome membrane; plasma membrane; integral to membrane
Molecular Function: protein binding; IgG binding; receptor signaling protein activity
Biological Process: antigen processing and presentation of peptide antigen via MHC class I; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; innate immune response; antigen processing and presentation of exogenous peptide antigen via MHC class I; immune response; signal transduction; phagocytosis, engulfment
Research Articles on CD64
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Product Notes
The CD64 fcgr1a (Catalog #AAA225859) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti Human CD64: Amethyst Orange reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD64 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: Min. Dilution: Neat Use 10ul of the suggested working dilution to label 106 cells in 100ul. Researchers should empirically determine the suitability of the CD64 fcgr1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD64, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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