Flow Cytometry (FC/FACS)
(Fig-1: Cell surface Flow analysis of hCD2 in Jurkat cells using 2 ug/ 10^6 cells. Green represents isotype control ;red represents anti-hCD28 antibody !0-4137). Goat anti-mouse PE conjugated secondary antibody was used. (Cells were incubated with primary antibody for 30 min. then washed twice with FLOW Staining buffer by centrifuging at 1100 rpm for 5 min, followed by 30 min incubation with conjugated secondary antibody. Data acquisition was done after washing twice with Staining buffer).)
Mouse anti-Human CD28 Monoclonal Antibody | anti-CD28 antibody
Monoclonal Antibody to CD28 (Clone: CB28)
Protein G Chromatography
Protein G Chromatography
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
Flow Cytometry (FC/FACS)
(Fig-1: Cell surface Flow analysis of hCD2 in Jurkat cells using 2 ug/ 10^6 cells. Green represents isotype control ;red represents anti-hCD28 antibody !0-4137). Goat anti-mouse PE conjugated secondary antibody was used. (Cells were incubated with primary antibody for 30 min. then washed twice with FLOW Staining buffer by centrifuging at 1100 rpm for 5 min, followed by 30 min incubation with conjugated secondary antibody. Data acquisition was done after washing twice with Staining buffer).)
Flow Cytometry (FC/FACS)
(Fig-1: Cell surface Flow analysis of hCD2 in Jurkat cells using 2 ug/ 10^6 cells. Green represents isotype control ;red represents anti-hCD28 antibody !0-4137). Goat anti-mouse PE conjugated secondary antibody was used. (Cells were incubated with primary antibody for 30 min. then washed twice with FLOW Staining buffer by centrifuging at 1100 rpm for 5 min, followed by 30 min incubation with conjugated secondary antibody. Data acquisition was done after washing twice with Staining buffer).)
Flow Cytometry (FC/FACS)
(Fig-2: Cell surface Flow analysis of CD28 in PBMC (lymphocytes gated) using 2 ug/ 10^6 cells. Green represents isotype control ;red represents anti-hCD28 antibody. Goat anti-mouse PE conjugated secondary antibody was used. (Cells were incubated with primary antibody for 30 min. then washed twice with FLOW Staining buffer by centrifuging at 1100 rpm for 5 min, followed by 30 min incubation with conjugated secondary antibody. Data acquisition was done after washing twice with Staining buffer).)
Flow Cytometry (FC/FACS)
(Fig-2: Cell surface Flow analysis of CD28 in PBMC (lymphocytes gated) using 2 ug/ 10^6 cells. Green represents isotype control ;red represents anti-hCD28 antibody. Goat anti-mouse PE conjugated secondary antibody was used. (Cells were incubated with primary antibody for 30 min. then washed twice with FLOW Staining buffer by centrifuging at 1100 rpm for 5 min, followed by 30 min incubation with conjugated secondary antibody. Data acquisition was done after washing twice with Staining buffer).)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]
Uniprot Description
CD28: a type I plasma membrane protein Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Binds to B7-1 and -2. Expressed in T cells and plasma cells, but not in less mature B cells. Six splice-variant isoforms have been described.
Protein type: Cell surface; Membrane protein, integral
Chromosomal Location of Human Ortholog: 2q33.2
Cellular Component: cell surface; cytosol; external side of plasma membrane; integral to plasma membrane; plasma membrane
Molecular Function: coreceptor activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protease binding; protein binding; SH3/SH2 adaptor activity
Biological Process: cell surface receptor linked signal transduction; cytokine biosynthetic process; humoral immune response; phosphoinositide-mediated signaling; positive regulation of interleukin-10 production; positive regulation of interleukin-2 biosynthetic process; positive regulation of interleukin-4 production; positive regulation of mitosis; positive regulation of T cell proliferation; regulation of defense response to virus by virus; regulation of phosphoinositide 3-kinase cascade; regulatory T cell differentiation; T cell costimulation
Research Articles on CD28
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Product Notes
The CD28 cd28 (Catalog #AAA668294) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to CD28 (Clone: CB28) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD28 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Flow Cytometry: 2-4 ug/10^6 cells. Researchers should empirically determine the suitability of the CD28 cd28 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD28, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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