Testing Data
(Recombinant Histone H3 MBS637639 (Lane 1) and HeLa acid extract (Lane 2) were resolved by electrophoresis, transferred to PVDF and probed with MBS618360 (0.5ug/ml). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Histone H3 at ~17kD.)
Rabbit anti-Human Histone H3 Polyclonal Antibody | anti-HIST3H3 antibody
Histone H3, dimethyl (Lys27) (MaxLight 750)
Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Chromatin Immunoprecipitation, Immunoprecipitation, Dot Blot
Purity
Purified by peptide affinity chromatography.
Synonyms
Histone H3; Polyclonal Antibody; dimethyl (Lys27) (MaxLight 750); anti-HIST3H3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human dimethylated histone H3 (Lys27), Mr 17kD. At high concentrations, some crossreactivity with dimethyl histone H3 (Lys9) occurs. Broad species crossreactivity expected, based on sequence homology.
Purity/Purification
Purified by peptide affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.
Applicable Applications for anti-HIST3H3 antibody
Western Blot (WB), Peptide Inhibition, Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP), Beadlyte Histone-Peptide Specificity Assay, Dot Blot (DB)
Application Notes
Applications are based on unconjugated antibody.
WB: 0.5ug/ml detects dimethylated histone H3 in acid extracted proteins from HeLa cells. No detection of 1ug unmethylated recombinant Histone H3 was observed.
WB: 0.5ug/ml detects dimethylated histone H3 in acid extracted proteins from HeLa cells. No detection of 1ug unmethylated recombinant Histone H3 was observed.
Immunogen
Synthetic branched peptide containing the sequence ARme2KSA... in which me2K corresponds to dimethyl-lysine at residue 27 of human histone H3 (KLH).
Conjugate
MaxLight750
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Testing Data
(Recombinant Histone H3 MBS637639 (Lane 1) and HeLa acid extract (Lane 2) were resolved by electrophoresis, transferred to PVDF and probed with MBS618360 (0.5ug/ml). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Histone H3 at ~17kD.)
Testing Data
(Recombinant Histone H3 MBS637639 (Lane 1) and HeLa acid extract (Lane 2) were resolved by electrophoresis, transferred to PVDF and probed with MBS618360 (0.5ug/ml). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Histone H3 at ~17kD.)
Testing Data
(12.5ng/ml (Figure B) of MBS618360 was incubated with a cocktail of microspheres conjugated to branched histone H3 peptides with the following modifications: 1. dimethyl-Histone H3 (Lys9) 2. monomethyl-Histone H3 (Lys27) 3. triimethyl-Histone H3 (Lys27) 4. dimethyl-Histone H3 (Lys27).)
Testing Data
(12.5ng/ml (Figure B) of MBS618360 was incubated with a cocktail of microspheres conjugated to branched histone H3 peptides with the following modifications: 1. dimethyl-Histone H3 (Lys9) 2. monomethyl-Histone H3 (Lys27) 3. triimethyl-Histone H3 (Lys27) 4. dimethyl-Histone H3 (Lys27).)
Testing Data
(Beadlyte Histone Peptide Specificity Assay: A range of dilutions (Figure C) of MBS618360 was incubated with a cocktail of microspheres conjugated to branched histone H3 peptides with the following modifications: 1. dimethyl-Histone H3 (Lys9) 2. monomethyl-Histone H3 (Lys27) 3. triimethyl-Histone H3 (Lys27) 4. dimethyl-Histone H3 (Lys27))
Testing Data
(Beadlyte Histone Peptide Specificity Assay: A range of dilutions (Figure C) of MBS618360 was incubated with a cocktail of microspheres conjugated to branched histone H3 peptides with the following modifications: 1. dimethyl-Histone H3 (Lys9) 2. monomethyl-Histone H3 (Lys27) 3. triimethyl-Histone H3 (Lys27) 4. dimethyl-Histone H3 (Lys27))
Product Categories/Family for anti-HIST3H3 antibody
References
Silva, J., et al., Dev. Cell 4: 481-495, 2003.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
136
NCBI Official Full Name
histone H3.1t
NCBI Official Synonym Full Names
histone cluster 3, H3
NCBI Official Symbol
HIST3H3
NCBI Official Synonym Symbols
H3t; H3.4; H3/g; H3FT
NCBI Protein Information
histone H3.1t; H3/t; histone 3, H3; H3 histone family, member T
UniProt Protein Name
Histone H3.1t
Protein Family
UniProt Gene Name
HIST3H3
UniProt Synonym Gene Names
H3FT; H3/t; H3t
UniProt Entry Name
H31T_HUMAN
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Product Notes
The HIST3H3 hist3h3 (Catalog #AAA6480764) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H3, dimethyl (Lys27) (MaxLight 750) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Peptide Inhibition, Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP), Beadlyte Histone-Peptide Specificity Assay, Dot Blot (DB). Applications are based on unconjugated antibody. WB: 0.5ug/ml detects dimethylated histone H3 in acid extracted proteins from HeLa cells. No detection of 1ug unmethylated recombinant Histone H3 was observed. Researchers should empirically determine the suitability of the HIST3H3 hist3h3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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