Rabbit anti-Mouse, Rat N/H/K-Ras Polyclonal Antibody | anti-N/H/K-Ras antibody

N/H/K-Ras Polyclonal Antibody

Cat. Num. AAA2556330

• Gene Names: Kras; p21; Kras2; c-Ki-ras
• Reactivity: Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purification
• Synonyms: N/H/K-Ras; Polyclonal Antibody; N/H/K-Ras Polyclonal Antibody; NRAS; HRAS1; GTPase NRas; Transforming protein N-Ras; HRAS; GTPase HRas; H-Ras-1; Ha-Ras; Transforming protein p21; c-H-ras; p21ras; KRAS; KRAS2; RASK2; GTPase KRas; K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras; anti-N/H/K-Ras antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Form/Format: PBS with 0.02% sodium azide, 100 mug/ml BSA and 50% glycerol.
• Concentration: 0.58 mg/mL (varies by lot)
• Sequence Length: 188

• Applicable Applications for anti-N/H/K-Ras antibody: Western Blot (WB)
• Application Notes: WB: 1:800-1:1000
• Immunogen: KLH conjugated synthetic peptide corresponding to Mouse K-Ras
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of various samples using N/H/K-Ras Polyclonal Antibody at dilution of 1:800.)

Related Product Information for anti-N/H/K-Ras antibody

NRAS an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. HRAS belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. KRAS, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.

Product Categories/Family for anti-N/H/K-Ras antibody

Cancer; Epigenetics and Nuclear Signaling; Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 13928698
• NCBI GeneID: 24525
• NCBI Accession #: NP_113703.1
• NCBI GenBank Nucleotide #: NM_031515.3
• UniProt Accession #: P08644; P46203; P97914
• Molecular Weight: Calculated: 21kDa; Observed: 21kDa
• NCBI Official Full Name: GTPase KRas
• NCBI Official Synonym Full Names: KRAS proto-oncogene, GTPase
• NCBI Official Symbol: Kras
• NCBI Official Synonym Symbols: p21; Kras2; c-Ki-ras
• NCBI Protein Information: GTPase KRas
• UniProt Protein Name: GTPase KRas
• UniProt Gene Name: Kras
• UniProt Synonym Gene Names: Kras2

NCBI Description

oncogene and member of the small GTPase superfamily [RGD, Feb 2006]

Uniprot Description

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (). Plays an important role in the regulation of cell proliferation (PubMed:3110778). Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner ().