Mouse anti-Human CD28 Monoclonal Antibody | anti-CD28 antibody

Monoclonal Antibody to CD28 (Clone: CB28) FITC Conjugated

Cat. Num. AAA668997

• Gene Names: CD28; Tp44
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Purified; Protein G Chromatography
• Synonyms: CD28; Monoclonal Antibody; Monoclonal Antibody to CD28 (Clone: CB28) FITC Conjugated; T-cell-specific surface glycoprotein CD28; TP44; anti-CD28 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, Kappa
• Clone Number: CB28
• Purity/Purification: Purified; Protein G Chromatography
• Form/Format: 0.2 mg/ml in Tris buffer containing 0.05% Azide
• Sequence Length: 123

• Applicable Applications for anti-CD28 antibody: Flow Cytometry (FC/FACS)
• Application Notes: FC/FACS: 0.2-0.5 ug/10^6 cells
• Antigen: CD28
• Immunogen Information: A partial length recombinant protein from CD28 was used as the immunogen for this antibody.
• Conjugation: FITC
• Preparation and Storage: Store the antibody at 4 degree C, stable for 6 months.

Flow Cytometry (FC/FACS)

(Fig-1: Cell surface Flow analysis of CD28 in PBMC (lymphocytes gated) using 0.2ug/ 10^6 cells. Green represents FITC conjugated isotype control;red represents FITC conjugated anti-hCD28 antibody (10-4137-F).)

Related Product Information for anti-CD28 antibody

CD28 is a type I transmembrane protein that binds through its extracellular region to B7 proteins (CD80 and CD86), which are transmembrane proteins expressed on the surface of APCs (Antigen-Presenting Cells) and are up-regulated by inflammatory signals. CD28 mediates signals that promote T lymphocyte differentiation and proliferation, and enhance antibody production of B lymphocytes. Deficiencies in CD28 pathways result in complete T lymphocyte tolerance in vitro and in vivo. Both CD4+ and CD8+ memory T cells need CD28 costimulation to achieve maximal expansion and pathogen clearance. The blockade of the CD28-B7 interaction has been used to down-regulate the activation of the immune system in autoimmune diseases. Based on the expression of the costimulatory molecule CD28 on the surface of CD8+ T cells, two different lymphocyte subgroups have been designated: antigen-primed cytotoxic T cells (CD8+CD28+ T cells) and suppressor T cells (CD8+CD28T cells). The frequency of CD28+CD8+T cells and especially the balance between CD8+CD28+ and CD8+CD28T cells are important in many diseases, including CHB (Chronic Hepatitis B).

Product Categories/Family for anti-CD28 antibody

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 340545508
• NCBI GeneID: 940
• NCBI Accession #: NP_001230006.1
• NCBI GenBank Nucleotide #: NM_001243077.1
• UniProt Accession #: P10747; Q13964; Q52M23; Q70WG0; Q8NI54; Q8NI55; Q8NI56; Q8WXJ2; Q9BYV0; A8KAC1
• Molecular Weight: 26,186 Da
• NCBI Official Full Name: T-cell-specific surface glycoprotein CD28 isoform 2
• NCBI Official Synonym Full Names: CD28 molecule
• NCBI Official Symbol: CD28
• NCBI Official Synonym Symbols: Tp44
• NCBI Protein Information: T-cell-specific surface glycoprotein CD28
• UniProt Protein Name: T-cell-specific surface glycoprotein CD28
• Protein Family: T-cell-specific surface glycoprotein
• UniProt Gene Name: CD28

NCBI Description

The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]

Uniprot Description

Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation (PubMed:8617933). Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed:15067037).