CD28 active protein

CD28, 19-152aa, Human, hIgG-His-tag, Baculovirus

Cat. Num. AAA206653

• Gene Names: CD28; Tp44
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: CD28; 19-152aa; Human; hIgG-His-tag; Baculovirus; T-cell-specific surface glycoprotein CD28 isoform 1; Tp44; CD28 active protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 1mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: ADPNKILVKQ SPMLVAYDNA VNLSCKYSYN LFSREFRASL HKGLDSAVEV CVVYGNYSQQ LQVYSKTGFN CDGKLGNESV TFYLQNLYVN QTDIYFCKIE VMYPPPYLDN EKSNGTIIHV KGKHLCPSPL FPGPSKPLEP KSCDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSRDE LTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK HHHHHH
• Sequence Length: 220

• Applicable Applications for CD28 active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: hIgG-His-tag
• Endotoxin Level: < 1.0 EU per 1ug of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for CD28 active protein

CD28, also known as T-cell-specific surface glycoprotein CD28 isoform 1, is one of the proteins expressed on T cells that provide co-stimulatory signals required for T cell activation and survival. T cell stimulation through CD28 in addition to the T-cell receptor (TCR) can provide a potent signal for the production of various interleukins (IL-6 in particular). CD28/B7 interaction has been shown to prevent apoptosis of activated T cells via the up-regulation of bcl-XL. It ligation has also been shown to regulate Th1/Th2 differentiation. Recombinant human CD28, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for CD28 active protein

Immunology

References

Prasad KV., et al. (1994) National Academy. 91: 2834 -2838.; Okkenhaug K., et al. (1998) J. Biol. Chem. 273: 21194 -21202.

NCBI and Uniprot Product Information

• NCBI GI #: 5453611
• NCBI GeneID: 940
• NCBI Accession #: NP_006130
• NCBI GenBank Nucleotide #: NM_006139.3
• UniProt Accession #: P10747; Q13964; Q52M23; Q70WG0; Q8NI54; Q8NI55; Q8NI56; Q8WXJ2; Q9BYV0; A8KAC1
• Molecular Weight: 42.4kDa (376aa) 40-57kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: T-cell-specific surface glycoprotein CD28 isoform 1
• NCBI Official Synonym Full Names: CD28 molecule
• NCBI Official Symbol: CD28
• NCBI Official Synonym Symbols: Tp44
• NCBI Protein Information: T-cell-specific surface glycoprotein CD28
• UniProt Protein Name: T-cell-specific surface glycoprotein CD28
• Protein Family: T-cell-specific surface glycoprotein
• UniProt Gene Name: CD28

NCBI Description

The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]

Uniprot Description

Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation (PubMed:8617933). Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed:15067037).