CD28 recombinant protein

Human CD28 protein, Fc tag (Animal-Free)

Cat. Num. AAA9718997

• Gene Names: CD28; Tp44
• Purity: >94% as determined by SDS-PAGE
• Synonyms: CD28; Human CD28 protein; Fc tag (Animal-Free); CD28 Molecule; T-Cell-Specific Surface Glycoprotein CD28; CD28 Antigen; T-Cell-Specific Surface Glycoprotein; CD28 Antigen (Tp44); Tp44; TP44; CD28 recombinant protein

• Host: HEK 293 Cells
• Purity/Purification: >94% as determined by SDS-PAGE
• Form/Format: 0.22um filtered protein solution is in PBS, pH7.4.
• Sequence: Asn19-Pro152

• Endotoxin: <0.1 EU per ug of the protein as determined by the LAL method.
• Protein Construction: Amino acid sequence derived from human CD28 protein (P10747-1, Asn19-Pro152) was expressed with the Fc region of human IgG1 at the C-terminus.
• Protein Length: The recombinant human CD28 protein consists of 371 amino acids and predicts a molecular mass of 42 kD. The apparent molecular mass of the human CD28 is approximately 53-70kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Usage Notes: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized human CD28 protein using the buffer we provided not less than 100ug/mL, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Lyophilized human CD28 protein product should be stored desiccated below -20 degree C. Upon reconstitution, the protein should be stored at 4 degree C between 2-7 days and for future use below -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

SDS-Page

(Fig.SDS-PAGE analysis of Human CD28 protein.)

Related Product Information for CD28 recombinant protein

Description: Recombinant Human CD28 protein expressed in HEK293 Cells.
Background: The cluster of differentiation (CD) antigen CD28 is a 44 kDa, disulphide-bonded, homodimeric glycoprotein, which is a costimulatory molecule expressed on the surface of T lymphocytes and plays an important role in the activation of T cells. It mediates the co stimulation of T cells by binding to B7 molecules on APC (antigen presenting cells). CD28 signals play a key role in many T cell processes, including cytoskeletal remodeling, production of cytokines, survival, and differentiation. Blocking CD28/B7 interactions may be useful in preventing unwanted activation in allergy and autoimmune diseases, whereas enhancing this interaction can promote tumour rejection. Thus, CD28 and its signalling pathways may prove to be useful targets in the development of new therapeutic treatments.

Product Categories/Family for CD28 recombinant protein

Cell Culture; Cytokines (Animal-Free)

NCBI and Uniprot Product Information

• NCBI GI #: 340545508
• NCBI GeneID: 940
• NCBI Accession #: NP_001230006.1
• NCBI GenBank Nucleotide #: NM_001243077.1
• UniProt Accession #: P10747; Q13964; Q52M23; Q8NI54; Q8NI55; Q8NI56; Q8WXJ2; Q9BYV0; A8KAC1
• Molecular Weight: 25,066 Da
• NCBI Official Full Name: T-cell-specific surface glycoprotein CD28 isoform 2
• NCBI Official Synonym Full Names: CD28 molecule
• NCBI Official Symbol: CD28
• NCBI Official Synonym Symbols: Tp44
• NCBI Protein Information: T-cell-specific surface glycoprotein CD28; CD28 antigen
• UniProt Protein Name: T-cell-specific surface glycoprotein CD28
• Protein Family: T-cell-specific surface glycoprotein
• UniProt Gene Name: CD28
• UniProt Entry Name: CD28_HUMAN

NCBI Description

The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]

Uniprot Description

CD28: a type I plasma membrane protein Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Binds to B7-1 and -2. Expressed in T cells and plasma cells, but not in less mature B cells. Six splice-variant isoforms have been described.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: integral to plasma membrane; plasma membrane; immunological synapse; cytosol; external side of plasma membrane

Molecular Function: identical protein binding; protein binding; SH3/SH2 adaptor activity; protease binding; coreceptor activity

Biological Process: positive regulation of isotype switching to IgG isotypes; positive regulation of translation; nerve growth factor receptor signaling pathway; viral reproduction; positive regulation of interleukin-2 biosynthetic process; cytokine biosynthetic process; T cell receptor signaling pathway; positive regulation of inflammatory response to antigenic stimulus; cell surface receptor linked signal transduction; positive regulation of T cell proliferation; regulatory T cell differentiation; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of viral genome replication; positive regulation of mitosis; negative thymic T cell selection; regulation of defense response to virus by virus; humoral immune response; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; T cell costimulation; innate immune response; positive regulation of alpha-beta T cell proliferation; positive regulation of transcription from RNA polymerase II promoter