Mouse anti-Human CD166 Monoclonal Antibody | anti-CD166 antibody

MOUSE ANTI HUMAN CD166

Cat. Num. AAA225953

• Reactivity: Human
• Applications: Immunoprecipitation, Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry
• Synonyms: CD166; Monoclonal Antibody; MOUSE ANTI HUMAN CD166; CD166 antibody|3A6; anti-CD166 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3A6
• Specificity: CD166
• Form/Format: Purified IgG, liquid. Phosphate buffered saline; Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
• Concentration: IgG concentration 1.0mg/ml (varies by lot)

• Applicable Applications for anti-CD166 antibody: Immunoprecipitation (IP), Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC)-Frozen
• Application Notes: FC/FACS: 1/10-1/50; FC/FACS: Use 10ul of the suggested working dilution to label 106 cells in 100ul.; Flow Cytometry: Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
• Species Cross-Reactivity: Sheep
• Immunogen: Human thymic epithelial cells.
• Preservative Stabilizers: 0.09% Sodium Azide
• Fusion Partners: Spleen cells from immunised mice were fused with cells of the P3X63 Ag8 myeloma cell line
• Carrier Free: Yes
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred.; This product should be stored undiluted.; Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.; Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Flow Cytometry (FC/FACS)

(Flow cytometric staining of Jurkat cells with preservative free Mouse anti Human CD166 antibody, clone 3A6 , filled histogram, with Mouse IgG isotype control , open histogram.)

Related Product Information for anti-CD166 antibody

Mouse anti Human CD166 antibody, clone 3A6 recognizes the 100 kDa adhesion molecule CD166, also known as ALCAM. CD166 is a member of the Ig superfamily and is expressed on activated T-cells, B cells and other cells including thymic epithelial cells, fibroblasts, keratinocytes and neurons. CD6 has been identified as a receptor for ALCAM (Skonier et al. 1996).
Mouse anti Human CD166 antibody, clone 3A6 is reported to cross-react with CD166 on ovine tissues and provides a useful tool for the identification and characterization of ovine mesenchymal stem cells in conjunction with CD44 which is expressed by this cell lineage and the hematopoietic cell marker CD45 which is not expressed on mesenchymal stem cells (Sanjurjo-Rodríguez et al. 2017).

References

1. Yeh, S.P. et al. (2005) Mesenchymal stem cells can be easily isolated from bone marrow of patients with various haematological malignancies but the surface antigens expression may be changed after prolonged ex vivo culture.Leukemia. 19: 1505-7.
2. Patel, D. D. et al. (1997) CD166 Workshop: Tissue distribution and functional analysis of antibodies reactive for CD166, a ligand for CD6.In Leukocyte Typing IV. Kishimoto, T. et al. eds Garland publishing Inc. New York p. 461-4.
3. Tondreau, T. et al. (2008) Gene expression pattern of functional neuronal cells derived from human bone marrow mesenchymal stromal cells.BMC Genomics. 9:166.
4. Wang, D. et al. (2004) Proteomic profiling of bone marrow mesenchymal stem cells upon transforming growth factor beta1 stimulation.J Biol Chem. 279 (42): 43725-34.
5. Green, L.R. et al. (2011) Cooperative role for tetraspanins in adhesin-mediated attachment of bacterial species to human epithelial cells.Infect Immun. 79 (6): 2241-9.